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Ryan 2014 J Physiol
Coupling states LEAK  + , OXPHOS  +
Has abstract The present study aimed to compare ''in vi
The present study aimed to compare ''in vivo'' measurements of skeletal muscle mitochondrial respiratory capacity made using near-infrared spectroscopy (NIRS) with the current gold standard, namely in situ measurements of high-resolution respirometry performed in permeabilized muscle fibres prepared from muscle biopsies. Mitochondrial respiratory capacity was determined in 21 healthy adults ''in vivo'' using NIRS to measure the recovery kinetics of muscle oxygen consumption following a ∼15 s isometric contraction of the vastus lateralis muscle. Maximal ADP-stimulated (State 3) respiration was measured in permeabilized muscle fibres using high-resolution respirometry with sequential titrations of saturating concentrations of metabolic substrates. Overall, the ''in vivo'' and in situ measurements were strongly correlated (Pearson's ''r''= 0.61-0.74, all ''p''< 0.01). Bland-Altman plots also showed good agreement with no indication of bias. The results indicate that ''in vivo'' NIRS corresponds well with the current gold standard, in situ high-resolution respirometry, for assessing mitochondrial respiratory capacity.
essing mitochondrial respiratory capacity.  +
Has info [http://www.ncbi.nlm.nih.gov/pubmed/24951618 PMID: 24951618]  +
Has publicationkeywords Oxidative phosphorylation  + , Mitochondrial respiration  + , Muscle energetics  +
Has title Ryan TE, Brophy P, Lin CT, Hickner RC, Neufer PD (2014) Assessment of ''in vivo'' skeletal muscle mitochondrial respiratory capacity in humans by near-infrared spectroscopy: a comparison with in situ measurements. J Physiol 592:3231-41.  +
Instrument and method Oxygraph-2k  +
Mammal and model Human  +
MiP area Respiration  + , Instruments;methods  +
Pathways F  + , N  + , S  + , NS  +
Preparation Permeabilized tissue  +
Tissue and cell Skeletal muscle  +
Was published by MiPNetLab US NC Greenville Neufer PD +
Was published in journal J Physiol +
Was published in year 2014  +
Was written by Ryan TE + , Brophy P + , Lin CT + , Hickner RC + , Neufer PD +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
08:00:56, 8 November 2016  +
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