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Sant'Anna-Silva 2018 Front Oncol
Additional label Labels  + , 2018-02  +
Coupling states LEAK  + , ROUTINE  + , ET  +
Diseases Cancer  +
Has abstract Tumor cells are subjected to a broad range
Tumor cells are subjected to a broad range of selective pressures. As a result of the imposed stress, subpopulations of surviving cells exhibit individual biochemical phenotypes that reflect metabolic reprograming. The present work aimed at investigating metabolic parameters of cells displaying increasing degrees of metastatic potential. The metabolites present in cell extracts fraction of tongue fibroblasts and of cell lines derived from human tongue squamous cell carcinoma lineages displaying increasing metastatic potential (SCC9 ZsG, LN1 and LN2) were analyzed by <sup>1</sup>H NMR (nuclear magnetic resonance) spectroscopy. Living, intact cells were also examined by the non-invasive method of fluorescence lifetime imaging microscopy (FLIM) based on the auto fluorescence of endogenous NADH. The cell lines reproducibly exhibited distinct metabolic profiles confirmed by Partial Least-Square Discriminant Analysis (PLS-DA) of the spectra. Measurement of endogenous free and bound NAD(P)H relative concentrations in the intact cell lines showed that ZsG and LN1 cells displayed high heterogeneity in the energy metabolism, indicating that the cells would oscillate between glycolysis and oxidative metabolism depending on the microenvironment's composition. However, LN2 cells appeared to have more contributions to the oxidative status, displaying a lower NAD(P)H free/bound ratio. Functional experiments of energy metabolism, mitochondrial physiology, and proliferation assays revealed that all lineages exhibited similar energy features, although resorting to different bioenergetics strategies to face metabolic demands. These differentiated functions may also promote metastasis. We propose that lipid metabolism is related to the increased invasiveness as a result of the accumulation of malonate, methyl malonic acid, n-acetyl and unsaturated fatty acids (CH<sub>2</sub>)<sub>n</sub> in parallel with the metastatic potential progression, thus suggesting that the NAD(P)H reflected the lipid catabolic/anabolic pathways.
ted the lipid catabolic/anabolic pathways.  +
Has editor [[Kandolf G]]  + , [[Sant'Anna-Silva ACB]]  +
Has info [https://www.ncbi.nlm.nih.gov/pubmed/29456966 PMID: 29456966 Open Access]  +
Has publicationkeywords Cancer progression  + , Metabolic reprogramming  + , Metabolomics  + , Metastasis  + , Nuclear magnetic resonance  + , Oral squamous cell carcinoma  +
Has title Sant'Anna-Silva ACB, Santos GC, Campos SPC, Oliveira Gomes AM, Pérez-Valencia JA, Rumjanek FD (2018) Metabolic profile of oral squamous carcinoma cell lines relies on a higher demand of lipid metabolism in metastatic cells. Front Oncol 8:13.  +
Instrument and method Oxygraph-2k  +
Mammal and model Human  +
MiP area Respiration  +
Pathways ROX  +
Preparation Intact cells  +
Respiration and regulation Inhibitor  +
Tissue and cell Other cell lines  + , Fibroblast  +
Was published by MiPNetLab BR Rio de Janeiro Rumjanek FD +
Was published in journal Front Oncol +
Was published in year 2018  +
Was written by Bastos Sant'Anna Silva AC + , Santos GC + , Campos SPC + , Oliveira Gomes AM + , Rumjanek FD + , Perez Valencia JA +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
11:26:43, 21 August 2018  +
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