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Schoepf 2016 Abstract Mito Xmas Meeting Innsbruck
Additional label Labelled by author  + , Prostate cancer  +
Coupling states ET  + , LEAK  + , OXPHOS  +
Diseases Cancer  +
Event Poster  +
Has abstract Reprogramming of energy metabolism is a ha
Reprogramming of energy metabolism is a hallmark of cancer. Mutations in the mitochondrial DNA (mtDNA) might contribute to cancer development and progression. We analyzed mitochondrial respiration of fresh malignant and non-malignant prostate tissue samples obtained from 50 prostate cancer patients by high-resolution respirometry (HRR), determined mtDNA copy numbers by duplex qPCR, sequenced the whole mtDNAs using next-generation sequencing (NGS) and analyzed expression of mt-related genes in a subset of 16 cases by RNA-sequencing. HRR uncovered a shift of respiratory activity from the glutamate&malate to pyruvate and succinate-pathways. The mutation load was significantly higher in tumor tissue compared to the non-malignant counterpart. Heteroplasmy levels of potentially deleterious mutations in mtDNA genes correlated significantly with reduced NADH-linked respiratory capacity. RNA-seq revealed a signature of differentially expressed metabolic enzymes in tumors exhibiting a severe compared to a mild NADH-pathway mt-phenotype. The gene signature corresponded to observed altered substrates effects on respiration, e.g. increased pyruvate and citrate and decreased glutamate oxidation.
citrate and decreased glutamate oxidation.  +
Has title Mitochondrial function in primary prostate cancer.  +
Instrument and method Oxygraph-2k  +
Mammal and model Human  +
MiP area Patients  + , Respiration  + , mt-Medicine  + , mtDNA;mt-genetics  + , nDNA;cell genetics  +
Pathways N  + , Other combinations  + , S  +
Preparation Permeabilized tissue  +
Respiration and regulation Flux control  +
Was published by MiPNetLab AT Innsbruck MitoFit + , AT Innsbruck Gnaiger E + , AT Innsbruck Oroboros +
Was submitted in year 2016  +
Was submitted to event Mito Xmas Meeting 2016 Innsbruck AT +
Was written by Schoepf B + , Weissensteiner H + , Charoentong P + , Schaefer G + , Bukur V + , Fendt L + , Kronenberg F + , Gnaiger E + , Klocker H + , Trajanoski Z +
Categories Abstracts
Modification date
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13:48:03, 23 January 2019  +
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