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Stankova 2010 Toxicol In Vitro
Enzyme Complex I  + , Complex II;succinate dehydrogenase  +
Has abstract Thioacetamide (TAA) is a hepatotoxin frequ
Thioacetamide (TAA) is a hepatotoxin frequently used for experimental purposes which produces centrilobular necrosis after a single dose administration. In spite of the fact that oxidative stress seems to play a very important role in the mechanism of TAA-induced injury, the effect of TAA on hepatocytes in primary culture with respect to the influence on mitochondria has yet to be verified. Hepatocytes were incubated for 24h in a medium containing TAA (0-70 mmol/l). Glutathione content (GSH/GSSG), reactive oxygen species and malondialdehyde formation were assessed as markers of cell redox state. Toxicity was determined by lactate dehydrogenase leakage and WST-1 assay. The functional capacity of hepatocytes was evaluated from albumin and urea production. Mitochondrial metabolism was assessed by measuring mitochondrial membrane potential and oxygen consumption. Our results show that a profound decrease in the GSH level in hepatocytes precedes a sharp rise in endogenous ROS production. ROS production correlates with an increase in lipoperoxidation. Mitochondria are affected by TAA secondarily as a consequence of oxidative stress. Oxidation of the NADH-dependent substrates of respiratory Complex I is significantly more sensitive to the toxic action of TAA than oxidation of the flavoprotein-dependent substrate of Complex II. Mitochondria can also maintain their membrane potential better when they utilize succinate as a respiratory substrate. It appears that GSH should be depleted below a certain critical level in order to cause a marked increase in lipid peroxidation. Mitochondrial injury can then occur and cell death develops.
ry can then occur and cell death develops.  +
Has info [ PMID: 20600801]  +
Has publicationkeywords Hepatotoxicity  +
Has title Staňková P, Kučera O, Lotková H, Roušar T, Endlicher R, Cervinková Z (2010) The toxic effect of thioacetamide on rat liver ''in vitro''. Toxicol In Vitro 24:2097-2103.  +
Instrument and method Oxygraph-2k  +
Pathways N  + , S  +
Preparation Permeabilized cells  +
Respiration and regulation mt-Membrane potential  + , Fatty acid  +
Stress Oxidative stress;RONS  +
Tissue and cell Liver  +
Was published by MiPNetLab CZ Hradec Kralove Cervinkova Z + , CZ Pardubice Rousar T +
Was published in journal Toxicol In Vitro +
Was published in year 2010  +
Was written by Stankova P + , Kucera O + , Lotkova H + , Rousar T + , Endlicher R + , Cervinkova Z +
Categories Publications
Modification date
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08:19:42, 8 November 2016  +
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