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Zhao 2019 Basic Res Cardiol
Additional label Labels  + , 2019-02  +
Coupling states LEAK  + , ROUTINE  + , OXPHOS  + , ET  +
Enzyme Complex I  + , Complex II;succinate dehydrogenase  + , Complex III  + , Complex IV;cytochrome c oxidase  + , Complex V;ATP synthase  +
Has abstract Cardiomyocyte loss and cardiac fibrosis ar
Cardiomyocyte loss and cardiac fibrosis are the main characteristics of cardiac ischemia and heart failure, and mitochondrial function of cardiomyocytes is impaired in cardiac ischemia and heart failure, so the aim of this study is to identify fate variability of cardiomyocytes and cardiac fibroblasts with mitochondria inhibition and explore the underlying mechanism. The mitochondrial respiratory function was measured by using Oxygraph-2k high-resolution respirometry. The STAT3 expression and activity were evaluated by western blot. Cardiomyocytes and cardiac fibroblasts displayed different morphology. The mitochondrial respiratory function and the expressions of mitochondrial complex I, II, III, IV, and V of cardiac fibroblasts were lower than that of cardiomyocytes. Mitochondrial respiratory complex I inhibitor rotenone and H<sub>2</sub>O<sub>2</sub> (100 µM, 4 h) treatment induced cell death of cardiomyocyte but not cardiac fibroblasts. The function of complex I/II was impaired in cardiomycytes but not cardiac fibroblasts stimulated with H<sub>2</sub>O<sub>2</sub> (100 µM, 4 h) and in ischemic heart of mice. Rotenone and H<sub>2</sub>O<sub>2</sub> (100 µM, 4 h) treatment reduced STAT3 expression and activity in cardiomyocytes but not cardiac fibroblasts. Inhibition of STAT3 impaired mitochondrial respiratory capacity and exacerbated H<sub>2</sub>O<sub>2</sub>-induced cell injury in cardiomycytes but not significantly in cardiac fibroblasts. In conclusion, the different susceptibility of cardiomyocytes and cardiac fibroblasts to mitochondria inhibition determines the cell fate under the same pathological stimuli and in which STAT3 plays a critical role.
and in which STAT3 plays a critical role.  +
Has editor [[Plangger M]]  +
Has info [https://www.ncbi.nlm.nih.gov/pubmed/30767143 PMID: 30767143]  +
Has publicationkeywords Cardiac fibroblasts  + , Cardiomyocytes  + , H2O2  + , Mitochondria  + , STAT3  +
Has title Zhao J, Gao JL, Zhu JX, Zhu HB, Peng X, Jiang M, Fu Y, Xu J, Mao XH, Hu N, Ma MH, Dong DL (2019) The different response of cardiomyocytes and cardiac fibroblasts to mitochondria inhibition and the underlying role of STAT3. Basic Res Cardiol 114:12.  +
Instrument and method Oxygraph-2k  +
Mammal and model Rat  +
MiP area Respiration  +
Pathways N  + , S  + , NS  + , ROX  +
Preparation Intact cells  + , Permeabilized cells  +
Stress Ischemia-reperfusion  +
Tissue and cell Heart  + , Fibroblast  +
Was published in journal Basic Res Cardiol +
Was published in year 2019  +
Was written by Zhao J + , Gao JL + , Zhu JX + , Zhu HB + , Peng X + , Jiang M + , Fu Y + , Xu J + , Mao XH + , Hu N + , Ma MH + , Dong DL +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
12:41:42, 20 February 2019  +
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