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Zhou 2019 Int J Mol Sci
Additional label Labels  + , 2019-04  +
Coupling states OXPHOS  +
Has abstract Synthetic cathinones are popular psychoact
Synthetic cathinones are popular psychoactive substances that may cause skeletal muscle damage. In addition to indirect sympathomimetic myotoxicity, these substances could be directly myotoxic. Since studies in myocytes are currently lacking, the aim of the present study was to investigate potential toxicological effects by synthetic cathinones on C2C12 myoblasts (mouse skeletal muscle cell line). We exposed C2C12 myoblasts to 3-methylmethcathinone, 4-methylmethcathinone (mephedrone), 3,4-methylenedioxymethcathinone (methylone), 3,4-methylenedioxypyrovalerone (MDPV), alpha-pyrrolidinovalerophenone (α-PVP), and naphthylpyrovalerone (naphyrone) for 1 or 24 h before cell membrane integrity, ATP content, mitochondrial oxygen consumption, and mitochondrial superoxide production was measured. 3,4-Methylenedioxymethamphetamine (MDMA) was included as a reference compound. All investigated synthetic cathinones, as well as MDMA, impaired cell membrane integrity, depleted ATP levels, and increased mitochondrial superoxide concentrations in a concentration-dependent manner in the range of 50⁻2000 μM. The two pyrovalerone derivatives α-PVP and naphyrone, and MDMA, additionally impaired basal and maximal cellular respiration, suggesting mitochondrial dysfunction. Alpha-PVP inhibited complex I, naphyrone complex II, and MDMA complex I and III, whereas complex IV was not affected. We conclude that, in addition to sympathetic nervous system effects and strenuous muscle exercise, direct effects of some cathinones on skeletal muscle mitochondria may contribute to myotoxicity in susceptible synthetic cathinone drugs users.
sceptible synthetic cathinone drugs users.  +
Has editor [[Plangger M]]  +
Has info [https://www.ncbi.nlm.nih.gov/pubmed/30925718 PMID: 30925718 Open Access]  +
Has publicationkeywords Electron transport chain  + , Mitochondria  + , Skeletal muscle toxicity  + , Synthetic cathinones  +
Has title Zhou X, Luethi D, Sanvee GM, Bouitbir J, Liechti ME, Krähenbühl S (2019) Molecular toxicological mechanisms of synthetic cathinones on C2C12 myoblasts. Int J Mol Sci 20:E1561.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
MiP area Respiration  + , Pharmacology;toxicology  +
Pathways N  +
Preparation Permeabilized cells  +
Tissue and cell Skeletal muscle  +
Was published by MiPNetLab CH Basel Kraehenbuehl S +
Was published in journal Int J Mol Sci +
Was published in year 2019  +
Was written by Zhou X + , Luethi D + , Sanvee GM + , Bouitbir J + , Liechti ME + , Kraehenbuehl S +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
12:06:30, 3 April 2019  +
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