Startup O2k-Respirometer

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OROBOROS support
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Startup O2k-Respirometer


Template NextGen-O2k.jpg
O2k-Open Support


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

Startup O2k-Respirometer notitle.png

Startup O2k-Respirometer - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System, the O2k-Titration Set, and for performing high-resolution respirometry with reduced amounts of biological sample the O2k-sV-Module.


The O2k is a sole source apparatus with no other instruments meeting its specifications.


Reference: Product details and purchase information


O2k-FluoRespirometer



Additional resources

» Contents: O2k Manual DatLab 7
» Gnaiger_2008_POS


ChapterViewSectionLast update
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.
2008
Gnaiger 2014 MitoPathwaysBioblast pdf
O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp.
2014
Gnaiger 2020 MitoPathwaysBioblast pdf 5th edition in preparationGnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2 (in prep).2020
MiPNet07.08 User informationBioblast pdf » Versions
O2k-Manual
O2k-Manual: user information. PLEASE STUDY THIS MANUAL.
2017-11-03
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
2016-08-11
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
2018-11-18
MiPNet12.11 MitoRespirationBioblast pdf »Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
2014-02-20
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
2015-06-18
MiPNet19.18 O2k-Series G Core manualBioblast pdf »Versions
O2k-Manual
O2k-Core manual contents.
2016-08-08
MiPNet19.18B POS-serviceBioblast pdf »Versions
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.
2019-05-15
MiPNet19.18C DatLab 6: GuideBioblast pdf »Versions
O2k-Manual
DatLab-guide through the menus.
2016-08-24
MiPNet19.18D O2k-Series G and DatLab 6: CalibrationBioblast pdf »Versions
O2k-Protocols
O2k-calibration by DatLab 6.
2016-08-24
MiPNet19.18E O2 flux analysis - DatLab 6Bioblast pdf »Versions
O2k-Manual
Oxygen flux analysis: DatLab real-time.
2016-08-08
MiPNet21.16 DatLab 7 Innovations
About Oroboros
DatLab 7: innovations.
2019-09-23
MiPNet22.16 DatLab 7 Innovations: DL-ProtocolsBioblast pdf » Versions
O2k-Protocols
DatLab 7.4 Innovations: DL-Protocols
2019-09-13
MiPNet23.12 DL-Protocols helpBioblast pdf » Versions
O2k-Protocols
DatLab Protocols: Help DatLab 7.
2019-09-13
MiPNet24.06 Oxygen flux analysis - DatLab 7.4Bioblast pdf » Versions
O2k-Protocols
Oxygen flux analysis with DatLab 7.4.
2020-04-16
MiPNet24.08 Safranin Analysis TemplateBioblast pdf » Versions
O2k-Protocols
Excel template for safranin data analysis.
2020-05-13
MiPNet24.09 General Template for Mt-membrane Potential AnalysisBioblast pdf » Versions
O2k-Protocols
General template for mitochondiral membrane potential analysis.
2020-05-13
MiPNet25.14 TPP Analysis TemplateBioblast pdf » [https:.pdf Versions]
O2k-Protocols
Excel template for TPP data analysis.
2020-##-##
Pesta 2012 Methods Mol BiolBioblast pdf
PMID: 22057559
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.
2012


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry