Sumbalova 2018 Recent Advances Mitochondr Med Coenzyme Q10
|Sumbalová Z, Garcia-Souza LF, Cizmarova B, Volani C, Gnaiger E (2018) Analysis of mitochondrial function in human blood cells. In: Recent advances in mitochondrial medicine and Coenzyme Q10. Gvozdjáková A, Cornelissen G, Singh RB eds, Nova Sciences:255-68.|
Abstract: Respirometric analysis of mitochondrial function in human immune cells and platelets has become increasingly attractive, since such tests can be accomplished within a few hours and may thereby support early diagnosis of diseases with mitochondrial dysfunctions. Here we discuss methodological aspects of blood cell preparation, show examples of measurements of mitochondrial respiration in intact and permeabilized platelets and peripheral blood mononuclear cells, and give a short overview of the current literature on blood cell bioenergetics in health and disease.
• Keywords: Immune cells, Lymphocytes, Platelets, PBMC, Oxidative phosphorylation, Respirometry • Bioblast editor: Kandolf G • O2k-Network Lab: AT Innsbruck Oroboros, SK Bratislava Sumbalova Z, AT Innsbruck Gnaiger E
Labels: MiParea: Respiration
Organism: Human Tissue;cell: Blood cells Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k
Labels, 2018-02, PBMCs, MitoEAGLEPublication