Svab 2019 Neurochem Res

From Bioblast
Publications in the MiPMap
Svab G, Doczi J, Gerencser AA, Ambrus A, Gallyas F, SΓΌmegi B, Tretter L (2019) The mitochondrial targets of neuroprotective drug vinpocetine on primary neuron cultures, brain capillary endothelial cells, synaptosomes, and brain mitochondria. Neurochem Res 44:2435-47.

Β» PMID: 31535355

Svab G, Doczi J, Gerencser AA, Ambrus A, Gallyas F, Suemegi B, Tretter L (2019) Neurochem Res

Abstract: Vinpocetine is considered as neuroprotectant drug and used for treatment of brain ischemia and cognitive deficiencies for decades. A number of enzymes, channels and receptors can bind vinpocetine, however the mechanisms of many effects' are still not clear. The present study investigated the effects of vinpocetine from the mitochondrial bioenergetic aspects. In primary brain capillary endothelial cells the purinergic receptor-stimulated mitochondrial Ca2+ uptake and efflux were studied. Vinpocetine exerted a partial inhibition on the mitochondrial calcium efflux. In rodent brain synaptosomes vinpocetine (30 ΞΌM) inhibited respiration in uncoupler stimulated synaptosomes and decreased H2O2 release from the nerve terminals in resting and in complex I inhibited conditions, respectively. In isolated rat brain mitochondria using either complex I or complex II substrates leak respiration was stimulated, but ADP-induced respiration was inhibited by vinpocetine. The stimulation of oxidation was associated with a small extent of membrane depolarization. Mitochondrial H2O2 production was inhibited by vinpocetine under all conditions investigated. The most pronounced effects were detected with the complex II substrate succinate. Vinpocetine also mitigated both Ca2+-induced mitochondrial Ca2+-release and Ca2+-induced mitochondrial swelling. It lowered the rate of mitochondrial ATP synthesis, while increasing ATPase activity. These results indicate more than a single mitochondrial target of this vinca alkaloid. The relevance of the affected mitochondrial mechanisms in the anti ischemic effect of vinpocetine is discussed. β€’ Keywords: ATP synthesis, Calcium induced calcium release, Mitochondria, Neuroprotection, Oxygen consumption, Reactive oxygen species, Uncoupling, Vinpocetine β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: HU Budapest Tretter L

Labels: MiParea: Respiration, Pharmacology;toxicology 

Organism: Guinea pig  Tissue;cell: Nervous system  Preparation: Isolated mitochondria, Intact cells 

Regulation: Calcium  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S  HRR: Oxygraph-2k 

2019-09, Labels 

Cookies help us deliver our services. By using our services, you agree to our use of cookies.