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Oroboros Instruments

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Description TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). The TIP2k-Module includes TIP2k-Accessories: 4 Microinjection syringes, 1 Pkg. TIP2k-Needle Spacers with mounting tool, 1 TIP2k-Needle Safety Support and 1 Pkg. TIP2k Filter Papers, and the newest DatLab version.
Product ID 11100-03
Type O2k, O2k-Module, Microsyringe, O2k-FluoRespirometer
Link MiPNet12.10 TIP2k-manual, MiPNet04.05 Titration-Injection
TIP2k with 200 mm3 microsyringe.JPG

MitoPedia O2k and high-resolution respirometry: O2k hardware 

TIP2k-Module consists of:

TitleDescriptionProduct idProduct image
Titration-Injection microPumpTitration-Injection microPump (TIP2k) for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat). Included in the TIP2k-Module.31000-01
Microsyringe\200 mm3\TIP2kMicrosyringe\200 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 200 mm3 (µl), fixed injection needle with rounded tip, with spacers. Four units of this item are standard components of the TIP2k-Module.31220-01
Microsyringe200 mm3TIP2k.JPG
TIP2k-Needle SpacerTIP2k-Needle Spacers for microinjection TIP2k needle, silicone stops (200/Pkg.) with mounting tool. This item is a standard component of the TIP2k-Module.31310-01
80600-24 Spacers f. TIP2k needle.JPG
TIP2k Filter PapersTIP2k Filter Papers (package of 10) to be used for the stoppers during TIP2k titration for Instrumental oxygen background correction to avoid constant manual siphoning off of extruded liquid. One package is a standard component of the TIP2k-Module.31320-01
TIP2k Filter Papers.JPG
TIP2k-Needle Safety Support with cable guideTIP2k-Needle Safety Support with cable guide: for safe storage of TIP2k-needles and MultiSensor Modules calbes when not required during the experiment. This item is a standard component of the TIP2k-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module.31330-02
31330-01 3.jpg

O2k with TIP2k

» TIP2k-Manual
The TIP2k is fully supported by the O2k.

The TIP2k is placed at the top of the Oroboros O2k. It does not need any extra bench space.

  • Align the front legs of the TIP2k with the TIP2k-Spacer Bar on the front of the O2k if you use 200 µl syringes.
  • Align the rear ends of the TIP2k and O2k, if you use 500 µl syringes.

Technical characteristics

Titration: Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.

Injection: Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1. Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration".

  • DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.


» SOP: Handling microsyringes
  • Rinse the outside with water immediately after use.
  • Wash 3 x with last used solvent, rinse with EtOH.
  • Storage: ethanol (short), dry (longtime).
  • Rinse with pure solvent before use.

List of O2k-Publications: TIP2k