Talk:Brewer GJ

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High-resolution respirometry with attached neuronal cells

From: Mitochondria Interest Group [1] From Gregory J. Brewer

Sent: 26 September 2011 18:53

To: MITOCHONDRIA-L@LIST.NIH.GOV

Re: [MITOCHONDRIA-L] functional mitochondria isolation from primary cortical cell cultures

Content of this Email is solely the Responsibility of the Author.--------


Dr. Castello and MIG,


It is challenging to isolate from cultured cells enough mitochondria for high-resolution respirometry. Then you will always wonder how the mitochondria were affected by the isolation and whether you obtained a representative fraction. Instead we developed techniques for high-resolution respirometry with the OROBOROS O2k of neuronal mitochondria in situ, with mitochondria in their normal environment inside neurons attached to a substrate. Here are our results:

Jones TT, Brewer GJ (2009) Critical age-related loss of cofactors of neuron cytochrome c oxidase reversed by estrogen. Exp Neurology 215: 212-9. »PM:18930048 PMCID:3018880

Jones TT, Brewer GJ (2010) Age-related deficiencies in Complex I endogenous substrate availability and reserve capacity of Complex IV in cortical neuron electron transport. Biochim Biophys Acta Bioenergetics 1797: 167-76. »PM:19799853 PMCID: 2812684


Please let me know if you need further assistance acquiring this information.

Grace to you,


Gregory J. Brewer, Ph.D., Professor

Kenneth Stark Endowed Chair in Alzheimer Research

Departments of Neurology and

Medical Microbiology, Immunology and Cell Biology

Southern Illinois University Sch. Med.

P.O. Box 19626

Springfield, IL 62794-9626

825 N. Rutledge Street, Room 2640

Springfield, IL 62702

gbrewer@siumed.edu

http://www.siumed.edu/mmi/faculty/brewer.html

http://www.BrainBitsLLC.com

217-545-5230

FAX 217-545-3227