Talk:Gnaiger 2022 EBEC
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Link: EBEC 2022 Conference website
Gnaiger E (2022)
Event: EBEC2022 Marseille FR
Cell respiration reflects mitochondrial functional fitness and plays a pivotal role in health and disease. Despite the rapidly increasing number of applications of cell respirometry to address current challenges in biomedical research, cross-references are rare between respirometric projects and platforms. Evaluation of accuracy and reproducibility between laboratories requires presentation of results in a common format independent of the applied method [1]. Normalization is mandatory for comparability of results [2]. Concept-driven normalization and regression analysis are key towards bioenergetic cluster analysis BCA, presented as a graphical tool to identify discrete data populations [3].
In a meta-analysis of human skin fibroblasts, high-resolution respirometry and polarography covering cell senescence and the human age range were compared with multiwell respirometry to evaluate published data on mitochondrial function in relation to clinical diagnostic standards. The common coupling control protocol measures ROUTINE respiration of living cells followed by sequential titrations of oligomycin, uncoupler, and inhibitors of electron transfer [4].
Bioenergetic cluster analysis BCA increases the resolution of outliers within and differences between groups. Isolinear clusters are separated by variations in quantities that correlate with rates, whereas heterolinear clusters fall on different regression lines. Dispersed clusters are clouds of data separated by a critical threshold value. BCA provides new insights into mitochondrial respiratory control and a guideline for establishing a quality control paradigm for bioenergetics and mitochondrial physiology. The present example on human fibroblasts as models of mitochondrial diseases may serve as a reference for bioenergetic mitochondrial diagnostics complementary to genomic or proteomic standards.
• Bioblast editor: Plangger M
• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck MitoFit, AT Innsbruck Oroboros
Affiliations and support
- Oroboros Instruments, Innsbruck, Austria. - erich.gnaiger@oroboros.at
- Supported by European Union’s Horizon 2020 grant 859770 NextGen-O2k; contribution to COST Action CA15203 MitoEAGLE.
References
- Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- Zdrazilova L, Hansikova H, Gnaiger E (2022) Comparable respiratory activity in attached and suspended human fibroblasts. PLoS ONE 17:e0264496. https://doi.org/10.1371/journal.pone.0264496
- Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.8. https://doi.org/10.26124/mitofit:2021-0008
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Respiration, Instruments;methods
Pathology: Aging;senescence
Organism: Human Tissue;cell: Fibroblast Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET
Pathway: ROX
HRR: Oxygraph-2k
