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Template:SUIT-029 O2 mt D066

From Bioblast
MitoPedia: SUIT

Steps and respiratory states

1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama.png


Step State Pathway Q-junction Comment - Events (E) and Marks (M)


1PM PML(n) N CI 1PM
2T PML or PMP N CI 1PM;2T
  • NADH-linked substrates (type N-pathway to Q).
  • In the absence of ATPase activity, the LEAK-state is maintained. However, if ATPases are active and thus generate ADP, respiration coupled to phosphorylation is stimulated. Stimulation is limited up to OXPHOS-capacity; therefore, higher ATPase activities cannot be determined.
2D PMP N CI 1PM;2T;2D
2c PMcP N CI 1PM;2T;2D;2c
3Omy PMLOmy N CI 1PM;2D;2c;3Omy
  • NADH-linked substrates (type N-pathway to Q).
  • Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
4U PME N CI 1PM;2T;2D;2c;3Omy;4U
5G PGME N CI 1PM;2T;2D;2c;3Omy;4U;5G
6S PGMSE NS CI+CII 1PM;2T;2D;2c;3Omy;4U;5G;6S
  • Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
  • Noncoupled electron transfer state, ET state, with ET capacity E.
6U PGMSE NS CI+CII 1PM;2T;2D;2c;3Omy;4U;5G;6S;6U
7Rot SE S CI 1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot
8Ama ROX 1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).


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