Template:SUIT text D007a

The SUIT-002 O2 ce-pce D007a protocol is the reference protocol 2 (RP2) specifically for PBMCs and PLTs. The main difference between the RP2 for PBMCs and PLTs and RP2 for other permeabilized cells (SUIT-002 O2 ce-pce D007) is the volume/concentration of the titrations. The SUIT-002 O2 ce-pce D007a protocol in combination with SUIT-001 O2 ce-pce D004 provides a common reference for comparison of respiratory control of PBMC and platelets cells in a wide variety of species. SUIT-002 O2 ce-pce D007a is specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. Moreover, the pathway control in OXPHOS state (F, F(N), FN, FNS, FNSGp pathways) and in ET state (FNSGp and SGp) can be evaluated by using this SUIT protocol. SUIT-002 O2 ce-pce D007a can be extended with the CIV assay module.