Template:SUIT text D091

The SUIT-038 O2 mt D091 protocol is focused on the analysis of the malate anaplerotic pathway control state in mitochondrial preparations such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of species, tissues and cell types. Malate alone does not support respiration if oxaloacetate is not metabolized further in the absence of acetyl-CoA. The careful titration of malate is required to analyze the activity of the mt-isoform of NADP- or NAD(P)-dependent malic enzyme (mtME). If these enzymes are present in the sample one should be able to reach with malate alone a high respiratory activity comparable to the NADH-linked pathway control states with more classical combinations of substrates (e.g. PM or GM). Moreover, the pathway control in OXPHOS state ((N), N, NS, NSGp pathways) and in ET state (NSGp and SGp) can be evaluated by using this SUIT protocol.