Thayer 1975 J Biol Chem
Thayer WS, Hinkle PC (1975) Synthesis of adenosine triphosphate by an artificially imposed electrochemical proton gradient in bovine heart submitochondrial particles. J Biol Chem 250:5330-5. |
Thayer WS, Hinkle PC (1975) J Biol Chem
Abstract: Submitochondrial particles subjected to an artificially imposed electrochemical proton gradient consisting of a pH gradient (acid to base transition) and membrane potential (low to high K-+ transition in the presence of valinomycin) catalyzed the net synthesis of 2.5 nmol of [-32P]ATP per mg of protein from ADP and 32-Pi. Optimal reaction conditions included incubation of submitochondrial particles in malonate at pH 5.0 with valinomycin in the absence of added K-+, followed by a rapid transition to pH 7.5 and 100 mM K-+. ATP synthesis continued for about 6 s and was sensitive to uncouplers or oligomycin but insensitive to inhibitors of electron transport. Lower amounts of ATP were formed by either the pH gradient (25%) of K-+ gradient (15%) alone. These results demonstrate that an electrochemical gradient of protons can drive the synthesis of ATP by reversal of the proton-translocating ATPase independent of electron transport. β’ Keywords: ATP synthesis, Proton gradient, Submitochondrial particles, Beef heart
Labels: MiParea: Respiration
Organism: Bovines
Tissue;cell: Heart
Preparation: SMP
Regulation: ATP production, Coupling efficiency;uncoupling, Ion;substrate transport, pH Coupling state: OXPHOS
Made history