A three-electrode system is the setup used in the Q-Sensor, which is an integral part of the Q-Module. This system is used in voltammetry (including cyclic voltammetry) to study the current as a function of the applied potential using three different electrodes: 1) the working electrode 2) the reference electrode, and 3) the counter electrode. The working or detecting electrode is a glassy carbon (GC) electrode that is set to a given potential and makes contact with the analyte. The potential of the working electrode is controlled by the constant potential of the a silver/silver chloride (Ag/AgCl) reference electrode, which does not pass any current. The applied potential on the surface of the GC should be sufficient to either oxidize reduced analyte (in our case Coenzyme Q) or to reduce oxidized CoQ. Thus, the counter electrode is a platinum electrode (Pt) that passes a current to counter these redox events by completing the circuit that is rate-limited by electron transfer on the GC. To determine the Q redox ratio the GC electrode is set at the oxidation peak potential, which can be determined with cyclic voltammetry.
The Q-Sensor is an integral part of the Q-Module and NextGen-O2k project
In the NextGen-O2k project we are working on developing the Q-Module for the new series of our O2k-FluoRespirometer, the NextGen-O2k. The Q-Module allows us to monitor the Q redox state of the Q-junction using the specific Q-Stoppers with the integrated three-electrode system and the modified electronics inside the O2k housing.
Communicated by Komlodi T, Cardoso LHD 2020-07-28
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Mitochondrial pathways, respiratory Complexes, and Q
NextGen-O2k and Q-Module