Timon-Gomez 2020 STAR Protoc

Timón-Gómez A, Pérez-Pérez R, Nyvltova E, Ugalde C, Fontanesi F, Barrientos A (2020) Protocol for the analysis of yeast and human mitochondrial respiratory chain complexes and supercomplexes by blue native electrophoresis. https://doi.org/10.1016/j.xpro.2020.100089

» STAR Protoc 1:100089. PMID: 32995753 Open Access

Timon-Gomez Alba, Perez-Perez Rafael, Nyvltova Eva, Ugalde Cristina, Fontanesi Flavia, Barrientos Antoni (2020) STAR Protoc

Abstract: By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from cellular or subcellular lysates while retaining their native conformation. BN-PAGE was first developed to analyze the size, composition, and relative abundance of the complexes and supercomplexes that form the mitochondrial respiratory chain and OXPHOS system. Here, we present a detailed protocol of BN-PAGE to obtain robust and reproducible results. For complete details on the use and execution of this protocol, please refer to Lobo-Jarne et al. (2018) and Timón-Gómez et al. (2020).

• Bioblast editor: Plangger M • O2k-Network Lab: US FL Miami Barrientos A

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2022-10