Toleikis 2005 Biosci Rep

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Toleikis A, Trumbeckaite S, Majiene D (2005) Cytochrome C effect on respiration of heart mitochondria: influence of various factors. Biosci Rep 25:387-97.

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Toleikis A, Trumbeckaite S, Majiene D (2005) Biosci Rep

Abstract: The effect of exogenous cytochrome c on respiration rate of the rat and human heart mitochondria was assessed in situ, using permeabilized fibers. It was (i) much more pronounced in State 2 and 4 than in State 3 with all the respiratory substrates (pyruvate+malate, succinate, palmitoyl-CoA+carnitine and octanoyl-L-carnitine), (ii) different with different substrates, (iii) much higher after ischemia in both metabolic states, particularly in the case of succinate oxidation compared to pyruvate+malate, (iv) the highest in State 4 with succinate as a substrate. Similar results were obtained with the isolated rat and rabbit heart mitochondria. The differences in the degree of stimulation of mitochondrial respiration by cytochrome c and, thus, sensitivity of cytochrome c test in evaluation of the intactness/injury of outer mitochondrial membrane are probably determined by the differences in the cytochrome c role in the control of mitochondrial respiration in the above-described conditions.

Keywords: Saponin-permeabilized heart muscle fibers, Isolated mitochondria, Outer mitochondrial membrane permeability, Cytochrome c test, Oxidative phosphorylation, Ischemia


Labels: MiParea: Respiration, Instruments;methods 

Stress:Ischemia-reperfusion  Organism: Human, Rat, Rabbit  Tissue;cell: Heart  Preparation: Permeabilized tissue, Isolated mitochondria 


Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S