Torelli 2015 Free Radic Biol Med

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Torelli NQ, Ferreira-Júnior JR, Kowaltowski AJ, da Cunha FM (2015) RTG1- and RTG2-dependent retrograde signaling controls mitochondrial activity and stress resistance in Saccharomyces cerevisiae. Free Radic Biol Med 81C:30-7.

» PMID:25578655

Torelli NQ, Ferreira-Junior JR, Kowaltowski AJ, da Cunha FM (2015) Free Radic Biol Med

Abstract: Mitochondrial retrograde signaling is a communication pathway between the mitochondrion and the nucleus which regulates the expression of a subset of nuclear genes that codify mitochondrial proteins, mediating cell response to mitochondrial dysfunction. In Saccharomyces cerevisiae, the pathway depends on Rtg1p and Rtg3p, which together form the transcription factor that regulates gene expression, and Rtg2p, an activator of the pathway. Here, we provide novel studies aimed at assessing the functional impact of the lack of RTG-dependent signaling on mitochondrial activity. We show that mutants defective in RTG dependent retrograde signaling present higher oxygen consumption and reduced hydrogen peroxide release in the stationary phase when compared to wild type cells. Interestingly, RTG mutants are less able to decompose hydrogen peroxide as well as maintain viability when challenged with hydrogen peroxide. Overall, our results indicate that RTG signaling is involved in the hormetic induction of antioxidant defenses and stress resistance.

Keywords: Retrograde signaling, Hormesis, Mitochondria, H2O2, Safranin, Amplex Red

O2k-Network Lab: BR Sao Paulo Kowaltowski AJ


Labels: MiParea: Respiration, mt-Membrane, Genetic knockout;overexpression 


Organism: Saccharomyces cerevisiae 

Preparation: Intact cells, Isolated mitochondria 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: CIV, ROX  HRR: Oxygraph-2k