Tsai 2016 Sci Rep

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Tsai HH, Chang SC, Chou CH, Weng TP, Hsu CC, Wang JS (2016) Exercise training alleviates hypoxia-induced mitochondrial dysfunction in the lymphocytes of sedentary males. Sci Rep 6:35170.

» PMID: 27731374 Open Access

Tsai HH, Chang SC, Chou CH, Weng TP, Hsu CC, Wang JS (2016) Sci Rep

Abstract: This study elucidates how interval and continuous exercise regimens affect the mitochondrial functionality of lymphocytes under hypoxic stress. Sixty healthy sedentary males were randomly assigned to engage in either high-intensity interval training (HIIT, 3 min intervals at 80% and 40% VO2max, n = 20) or moderate-intensity continuous training (MICT, sustained 60% VO2max, n = 20) for 30 min/day, 5 days/week for 6 weeks or were assigned to a control group that did not receive exercise intervention (n = 20). Lymphocyte phenotypes/mitochondrial functionality under hypoxic exercise (HE, 100 W under 12% O2) were determined before and after the various interventions. Before the intervention, HE (i) increased the mobilization of senescent (CD57+/CD28-) lymphocytes into the blood, (ii) decreased the ATP-linked O2 consumption rate (OCR), the reserve capacity of OCR, and the citrate synthase activity in the mitochondria, and (iii) lowered the mitochondrial membrane potential (MP) and elevated the matrix oxidant burden (MOB) of lymphocytes. However, both HIIT and MICT significantly (i) decreased blood senescent lymphocyte counts, (ii) enhanced the mitochondrial OCR with increased citrate synthase and succinate dehydrogenase activities, (iii) increased mitochondrial MP and decreased MOB and (iv) increased the ratio of mitofusin to DRP-1 in lymphocytes after HE. Thus, we concluded that either HIIT or MICT effectively improves lymphocyte mitochondrial functionality by enhancing oxidative phosphorylation and suppressing oxidative damage under hypoxic conditions.


O2k-Network Lab: TW Taoyuan Wang JS


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, Exercise physiology;nutrition;life style 

Stress:Oxidative stress;RONS  Organism: Human  Tissue;cell: Blood cells, Lymphocyte  Preparation: Intact cells, Permeabilized cells 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: F, N, S, NS, Other combinations, ROX  HRR: Oxygraph-2k 

2016-10 

» Figure 4 Graph showing measurement of mitochondrial O2 consumption rate (OCR) in lymphocytes using a high-resolution respirometry (Oroboros O2k).
Fig 2: Exercise induced changes in the lymphocytes phenotypes. Hypoxic exercise induced CD57-positive lymphocytes population to increase in both sedentary and trained individuals.
"Acute 12% O2 exercise increased the mobilization of senescent lymphocytes into the peripheral blood and resulted in elevated oxidative stress and decreased oxidative phosphorylation in lymphocyte mitochondria. Although no changes were found in mitochondrial biogenesis, both HIIT and MICT regimens effectively shifted lymphocyte mitochondria toward fusion, attenuated the HE-induced release of senescent subsets and lowered the mitochondrial OXPHOS capacity in lymphocytes."