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Tsien 1981 J Neurosci Methods

From Bioblast
Publications in the MiPMap
Tsien RY, Rink TJ (1981) Ca2+-selective electrodes: a novel PVC-gelled neutral carrier mixture compared with other currently available sensors. J Neurosci Methods 4(1):73-86.

Β» PMID: 7253701

Tsien RY, Rink TJ (1981) J Neurosci Methods

Abstract: The great importance of cytoplasmic free Ca2+ in regulating cellular function has stimulated the development of several more-or-less calcium-selective sensor systems for microelectrodes. The known formulations each have serious deficiencies, particularly in fine microelectrodes. We have developed a new sensor which gives distinctly improved performance in submicron tips. This sensor combines the widely used neutral carrier ETH1001 (Ammann et al., 1979) with an intensely hydrophobic salt into a poly(vinyl-chloride) gel. This study compares the calibration responses of the various sensor systems under a uniform set of experimental conditions. β€’ Keywords: calcium, microelectrodes β€’ Bioblast editor: Cecatto C


Labels: MiParea: Instruments;methods 




Regulation: Calcium 



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