User talk:Spitzer Gudrun

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Antimycin A version 1

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 5 mM stock solution (dissolved in 99.9% ethanol) for 2-mL O2k-chamber:
  1. Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
  2. Add 2 mL ethanol.
  3. Divide into 0.2 mL portions in clear glass vials.
  4. Store at -20 °C.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 2.5 µM.


Preparation of 1,25 mM stock solution (dissolved in 99.9% ethanol) for 0.5-mL O2k-chamber:
  1. Take 100 µL of 5 mM stock solution.
  2. Add 300 µL ethanol.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 2.5 µM.

Antimycin A version 2

Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
  1. Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
  2. Add 2 mL ethanol.
  3. Divide into 0.2 mL portions in clear glass vials.
  4. Store at -20 °C.
  5. For 1.25 mM concentration take 100 µL stock solution (5 mM) and dilute it with 300 µl ethanol.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 1 µL (5 mM) using a 10 µL Hamilton syringe.
  • Titration volume (0.5-mL O2k-chamber): 1 µL (1.25 mM) using a 10 µL Hamilton syringe.
  • Final concentration: 2.5 µM.