Vandenberg 2021 Neurochem Int

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Publications in the MiPMap
Vandenberg GG, Dawson NJ, Head A, Scott GR, Scott AL (2021) Astrocyte-mediated disruption of ROS homeostasis in Fragile X mouse model. Neurochem Int 146:105036.

» PMID: 33785420

Vandenberg Gregory G, Dawson Neal J, Head Alison, Scott Graham R, Scott Angela L (2021) Neurochem Int

Abstract: Astrocytes, glial cells within the brain, work to protect neurons during high levels of activity by maintaining oxidative homeostasis via regulation of energy supply and antioxidant systems. In recent years, mitochondrial dysfunction has been highlighted as an underlying factor of pathology in many neurological disorders. In animal studies of Fragile X Syndrome (FXS), the leading genetic cause of autism, higher levels of reactive oxygen species, lipid peroxidation, and protein oxidation within the brain indicates that mitochondria function is also altered in FXS. Despite their integral contribution to redox homeostasis within the CNS, the role of astrocytes on the occurrence or progression of neurodevelopmental disorders in this way is rarely considered. This study specifically examines changes to astrocyte mitochondrial function and antioxidant expression that may occur in FXS. Using the Fmr1 knockout (KO) mouse model, mitochondrial respiration and reactive oxygen species (ROS) emission were analyzed in primary cortical astrocytes. While mitochondrial respiration was similar between genotypes, ROS emission was significantly elevated in Fmr1 KO astrocytes. Notably, NADPH-oxidase 2 expression in Fmr1 KO astrocytes was also enhanced but only changes in catalase antioxidant enzyme expression were noted. Characterization of astrocyte factors involved in redox imbalance is invaluable to uncovering potential sources of oxidative stress in neurodevelopmental disorders and more specifically, the intercellular mechanisms that contribute to dysfunction in FXS. Keywords: Antioxidants, Astrocyte, Fragile X Syndrome, Mitochondrial respiration, Mouse model, Oxidative stress, Reactive species Bioblast editor: Reiswig R O2k-Network Lab: CA Hamilton Scott GR


Labels: MiParea: Respiration, Genetic knockout;overexpression  Pathology: Autism 

Organism: Mouse  Tissue;cell: Nervous system  Preparation: Permeabilized cells 


Coupling state: LEAK, OXPHOS  Pathway: N, CIV, NS  HRR: Oxygraph-2k, O2k-Fluorometer 

2021-07, AmR