Vicentini 2019 Plant Physiol Biochem

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Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on Jatropha curcas cell culture. Plant Physiol Biochem 136:92-97.

» PMID: 30660100

Vicentini Tatiane M, Cavalheiro Amanda H, Dechandt Carlos RP, Alberici Luciane Carla, Vargas-Rechia Carem G (2019) Plant Physiol Biochem

Abstract: Aluminum (Al) toxicity has been recognized to be a main limiting factor of crop productivity in acid soil. Al interacts with cell walls disrupting the functions of the plasma membrane and is associated with oxidative damage and mitochondrial dysfunction. Jatropha curcas L. (J. curcas) is a drought resistant plant, widely distributed around the world, with great economic and medicinal importance. Here we investigated the effects of Al on J. curcas mitochondrial function and cell viability, analyzing mitochondrial respiration, phenolic compounds, reducing sugars and cell viability in cultured J. curcas cells. The results showed that at 70 μM, Al limited mitochondrial respiration by inhibiting the alternative oxidase (AOX) pathway in the respiratory chain. An increased concentration of reducing sugars and reduced concentration of intracellular phenolic compounds was observed during respiratory inhibition. After inhibition, a time-dependent upregulation of AOX mRNA was observed followed by restoration of respiratory activity and reducing sugar concentrations. Cultured J. curcas cells were very resistant to Al-induced cell death. In addition, at 70 μM, Al also appeared as an inhibitor of cell wall invertase. In conclusion, Al tolerance in cultured J. curcas cells involves a inhibition of mitochondrial AOX pathway, which seems to start an oxidative burst to induce AOX upregulation, which in turn restores consumption of O2 and substrates. These data provide new insight into the signaling cascades that modulate the Al tolerance mechanism.

Copyright © 2019 Elsevier Masson SAS. All rights reserved.

Keywords: Alternative oxidase, Invertase activity, Jatropha curcas cell culture, Mitochondrial respiration, Phenolic compounds, Reducing sugars Bioblast editor: Plangger M O2k-Network Lab: BR Ribeirao Preto Alberici LC

Labels: MiParea: Respiration, Pharmacology;toxicology 

Organism: Plants 

Preparation: Intact cells 

Coupling state: ROUTINE 

HRR: Oxygraph-2k 

2019-01, AOX