Walczak 2018 FASEB J

From Bioblast
Publications in the MiPMap
Walczak J, DΔ™bska-Vielhaber G, Vielhaber S, SzymaΕ„ski J, CharzyΕ„ska A, DuszyΕ„ski J, Szczepanowska J (2018) Distinction of sporadic and familial forms of ALS based on mitochondrial characteristics. FASEB J 33:4388-403.

Β» PMID: 30550341

Walczak J, Debska-Vielhaber G, Vielhaber S, Szymanski J, Charzynska A, Duszynski J, Szczepanowska J (2018) FASEB J

Abstract: Bioenergetic failure, oxidative stress, and changes in mitochondrial morphology are common pathologic hallmarks of amyotrophic lateral sclerosis (ALS) in several cellular and animal models. Disturbed mitochondrial physiology has serious consequences for proper functioning of the cell, leading to the chronic mitochondrial stress. Mitochondria, being in the center of cellular metabolism, play a pivotal role in adaptation to stress conditions. We found that mitochondrial dysfunction and adaptation processes differ in primary fibroblasts derived from patients diagnosed with either sporadic or familial forms of ALS. The evaluation of mitochondrial parameters such as the mitochondrial membrane potential, the oxygen consumption rate, the activity and levels of respiratory chain complexes, and the levels of ATP, reactive oxygen species, and Ca2+ show that the bioenergetic properties of mitochondria are different in sporadic ALS, familial ALS, and control groups. Comparative statistical analysis of the data set (with use of principal component analysis and support vector machine) identifies and distinguishes 3 separate groups despite the small number of investigated cell lines and high variability in measured parameters. These findings could be a first step in development of a new tool for predicting sporadic and familial forms of ALS and could contribute to knowledge of its pathophysiology. β€’ Keywords: PCA, Amyotrophic lateral sclerosis, Neurodegeneration, Primary fibroblasts β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: DE Magdeburg Gellerich FN, PL Warsaw Szewczyk A

Labels: MiParea: Respiration, Patients  Pathology: Neurodegenerative 

Organism: Human  Tissue;cell: Fibroblast  Preparation: Permeabilized cells  Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase  Regulation: ATP  Coupling state: LEAK, ROUTINE, OXPHOS  Pathway: N, S  HRR: Oxygraph-2k 


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