Waskova-Arnostova 2015 J Appl Physiol (1985)
| Waskova-Arnostova P, Elsnicova B, Kasparova D, Hornikova D, Kolar F, Novotny J, Zurmanova J (2015) Cardioprotective adaptation of rats to intermittent hypobaric hypoxia is accompanied by the increased association of hexokinase with mitochondria. J Appl Physiol (1985) 119:1487-93. |
Waskova-Arnostova P, Elsnicova B, Kasparova D, Hornikova D, Kolar F, Novotny J, Zurmanova Jitka (2015) J Appl Physiol (1985)
Abstract: Chronic hypoxia increases the myocardial resistance to acute ischemia-reperfusion injury by affecting the mitochondrial redox balance. Hexokinase (HK) bears a high potential to suppress the excessive formation of reactive oxygen species due to its increased association with mitochondria, thereby inhibiting the membrane permeability transition pore opening and preventing cell death. The purpose of this study was to determine the effect of severe intermittent hypobaric hypoxia (7000 m, 8 h/day, 5 weeks) on the function and co-localization of HK isoforms with mitochondria in the left (LV) and right ventricles of rat myocardium. The Real-Time RT-PCR, Western blot, enzyme coupled assay, and quantitative immunofluorescence techniques were used. Our results showed significantly elevated expression of HK isoforms (HK1 and HK2) in the hypoxic LV. In addition, intermittent hypoxia increased the total HK activity and the association of HK isoforms with mitochondria in both ventricles. These findings suggest that HK may play a role in the cardioprotective phenotype induced by adaptation to severe intermittent hypobaric hypoxia. • Keywords: Cardioprotection, Chronic hypoxia, Hexokinase, Mitochondria co-localization, Rat heart
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Heart Preparation: Homogenate
