Wavelength averaging

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Bioblasts - Richard Altmann and MiPArt by Odra Noel
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Wavelength averaging


Wavelength averaging is the averaging of several adjacent data points across the recorded spectrum (spectral smoothing), to improve the signal-to-noise ratio. For example, if the instrument recorded 5 data points per nm, the average of the 5 points can be taken for each successive nm across the range of the spectrum to give a 5-point smoothing. This method clearly reduces the wavelength resolution.

MitoPedia methods: Fluorometry, Spectrophotometry