Wet weight of a tissue or biological sample, obtained after blotting the sample to remove an arbitrary amount of water adhering externally to the sample.
Wet weight determination
- Weight measurements are made after permeabilization of the tissue (either mechanical or chemical permeabilization), just before the sample is added to the chamber.
- This allows eliminating for:
- * variations in water contents due to osmotic stress
- * the connective tissue during mechanical separation
- * partitioning of subsamples of similar wet weight.
- Before adding the tissue into the O2k chamber, take wet weight measurements of several loosely connected fiber bundles of c. 1-3 mg wet weight (skeletal muscle) or 0.5-2 mg wet weight (heart). Blot the bundles carefully on filter paper. Take the sample with the sharp pair of forceps (angular tip) and place it for 5 s onto the filter paper. During this time, wipe off any liquid from the tip of the forceps with another filter paper. Then take the sample from the filter paper and touch it once more shortly onto a dry area of filter paper while holding it with the forceps. Then immediately place the sample onto a small plastic plate on the table of the tarred balance. Immediately after reading the wet weight, the sample is transferred into a well with 2 ml ice cold MiR06. Check that the tare balance reading returns to zero. Each well receives a sample for an O2k-chamber.