Willet 2000 Biochim Biophys Acta

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Willet K, Detry O, Sluse FE (2000) Resistance of isolated pulmonary mitochondria during in vitro anoxia/reoxygenation. Biochim Biophys Acta 1460:346-52.

» PMID: 11106775 Open Access

Willet K, Detry O, Sluse FE (2000) Biochim Biophys Acta

Abstract: The aim of the study was to investigate the effect of in vitro anoxia/reoxygenation on the oxidative phosphorylation of isolated lung mitochondria. Mitochondria were isolated after harvesting from fresh pig lungs flushed with Euro-Collins solution. Mitochondrial respiratory parameters were determined in isolated mitochondria before anoxia (control), after 5–45 min anoxia followed by 5 min reoxygenation, and after 25 or 40 min of in vitro incubation in order to follow the in vitro aging of mitochondria during respiratory assays. Respiratory parameters measured after anoxia/reoxygenation did not show any oxidative phosphorylation dysfunction, indicating a high resistance of pulmonary mitochondria to in vitro anoxia/reoxygenation (up to 45 min anoxia). These results indicate that mitochondria are not directly responsible of their oxidative phosphorylation damage observed after in vivo ischemia (K. Willet et al., Transplantation 69 (2000) 582) but are a target of others cellular injuries leading to mitochondrial dysfunction in vivo.

Keywords: Mitochondria, Anoxia, Reoxygenation, Oxidative phosphorylation, Transplantation, (Lung)

Labels: MiParea: Respiration 

Stress:Ischemia-reperfusion  Organism: Pig  Tissue;cell: Lung;gill  Preparation: Isolated mitochondria 

Coupling state: OXPHOS 

HRR: Oxygraph-2k