Wu 2017b FASEB J
|Effects of interval and continuous exercise regimens on platelet mitochondrial bioenergetics under hypoxic stress.|
Link: Open Access
Mitochondrial bioenergetics is an important in the regulation of platelet function. The purpose of this study was to investigate the effects of aerobic interval (AIT) and moderate continuous training (MCT) on mitochondrial oxidative phosphorylation and thrombin generation (TG) of platelets during hypoxic stress. Twenty-four sedentary males were randomized to perform either aerobic interval training (AIT; 3-minute intervals at 40% and 80% VO2max, n=12) or moderate continuous training (MCT; sustained 60% VO2max, n=12) for 30 minutes/day, 5 days/week for 6 weeks. At rest and immediately after hypoxic exercise test (HE, 100W under 12%O2 for 30 min), mitochondrial oxidative phosphorylation and dynamic TG were measured by a high resolution respirometer (Oxygraph-2k) and a calibrated automated thrombinography, respectively. The results demonstrated that AIT rather than MCT regimen significantly increased ATP-linked O2 consumption rate (OCR), Complex II-mediated OCR, and reserve capacity of OCR, which were accompanied by lowered dynamic TG in platelet-rich plasma. Moreover, AIT significantly diminished the extents of mitochondrial dysfunction and TG elevation caused by HE, compared to MCT did. Hence, we conclude that AIT is superior to MCT for improving platelet mitochondrial bioenergetics and suppressing thrombus formation at a hypoxic environment.
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Stress:Hypoxia Organism: Human Tissue;cell: Blood cells, Platelet
Pathway: S HRR: Oxygraph-2k