Zheleznova 2012 Physiol Genomics

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Zheleznova NN, Yang C, Ryan RP, Halligan BD, Liang M, Greene AS, Cowley AW Jr (2012) Mitochondrial proteomic analysis reveals deficiencies in oxygen utilization in medullary thick ascending limb of Henle in the Dahl salt-sensitive rat. Physiol Genomics 44:829-42.

» PMID: 22805345

Zheleznova NN, Yang C, Ryan RP, Halligan BD, Liang M, Greene AS, Cowley AW Jr (2012) Physiol Genomics

Abstract: The renal medullary thick ascending limb (mTAL) of the Dahl salt-sensitive rat (SS) is the site of enhanced NaCl reabsorption and excess superoxide production. In the present studies we isolated mitochondria from mTAL of SS and salt-resistant control strain SS.13(BN) rats on 0.4% and 8% salt diet for 7 days and performed a proteomic analysis. Purity of mTAL and mitochondria isolations were exceeded 93.6% and 55%, respectively. Using LC/MS spectral analysis techniques 96 mitochondrial proteins were identified in 4 biological mTAL mitochondria samples, run in duplicate, as defined by proteins with a false discovery rate <5% and scan count >2. Seven of these 96 proteins, including IDH2, ACADM, SCOT, Hsp60, ATPA, EFTu and VDAC2 were differentially expressed between the two rat strains. Oxygen consumption and high resolution respirometry analyses showed that mTAL cells and the mitochondria in the outer medulla of SS rats fed high salt diet exhibited lower rates of oxygen utilization compared to those from SS.13(BN) rats. These studies advance the conventional proteomic paradigm of focusing exclusively upon whole tissue homogenates to a focus upon a single cell type and specific subcellular organelle. The results reveal the importance of a largely unexplored role for deficiencies of mTAL mitochondrial metabolism and oxygen utilization in salt-induced hypertension and renal medullary oxidative stress.

Keywords: high salt diet, mitochondrial metabolism, oxygen utilization, salt-induced hypertension, renal medullary oxidative stress

O2k-Network Lab: NZ Auckland Hickey AJ


Labels: MiParea: Respiration 

Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Kidney  Preparation: Isolated mitochondria 

Regulation: Ion;substrate transport 


HRR: Oxygraph-2k