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Zulkifli 2020 Nat Commun

From Bioblast
Publications in the MiPMap
Zulkifli M, Neff JK, Timbalia SA, Garza NM, Chen Y, Watrous JD, Murgia M, Trivedi PP, Anderson SK, Tomar D, Nilsson R, Madesh M, Jain M, Gohil VM (2020) Yeast homologs of human MCUR1 regulate mitochondrial proline metabolism. Nat Commun 11:4866.

Β» PMID: 32978391 Open Access

Zulkifli Mohammad, Neff John K, Timbalia Shrishiv A, Garza Natalie M, Chen Yingqi, Watrous Jeramie D, Murgia Marta, Trivedi Prachi P, Anderson Steven K, Tomar Dhanendra, Nilsson Roland, Madesh Muniswamy, Jain Mohit, Gohil Vishal M (2020) Nat Commun

Abstract: Mitochondria house evolutionarily conserved pathways of carbon and nitrogen metabolism that drive cellular energy production. Mitochondrial bioenergetics is regulated by calcium uptake through the mitochondrial calcium uniporter (MCU), a multi-protein complex whose assembly in the inner mitochondrial membrane is facilitated by the scaffold factor MCUR1. Intriguingly, many fungi that lack MCU contain MCUR1 homologs, suggesting alternate functions. Herein, we characterize Saccharomyces cerevisiae homologs Put6 and Put7 of MCUR1 as regulators of mitochondrial proline metabolism. Put6 and Put7 are tethered to the inner mitochondrial membrane in a large hetero-oligomeric complex, whose abundance is regulated by proline. Loss of this complex perturbs mitochondrial proline homeostasis and cellular redox balance. Yeast cells lacking either Put6 or Put7 exhibit a pronounced defect in proline utilization, which can be corrected by the heterologous expression of human MCUR1. Our work uncovers an unexpected role of MCUR1 homologs in mitochondrial proline metabolism.

β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: US TX College Station Gohil VM


Labels: MiParea: Respiration, mt-Membrane, nDNA;cell genetics 


Organism: Saccharomyces cerevisiae 

Preparation: Intact cells 


Coupling state: ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2020-10