Difference between revisions of "ATPases"
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|description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to production of ADP, which can stimulate respiration. This situation would lead to an | |description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of [[LEAK-respiration]] measured in the absence of ADP, ''L''(n) and subsequent inhibition of respiration by oligomycin, ''L''(Omy). | ||
|info= | |info=[[LEAK-respiration]] | ||
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{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Enzyme | |mitopedia topic=Enzyme | ||
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== [[SUITbrowser]] question: Unspecific ATPase activity == | |||
:::: [[SUIT]] protocols can be used to analyze the presence of contaminating ATPases in the sample. The [[SUIT-024]] is focused on answering this question. | |||
:::: The [https://suitbrowser.oroboros.at/ SUITbrowser] was developed for searching for the best SUIT protocols to answer this and other research questions. | |||
Revision as of 09:52, 3 July 2019
Description
ATPases are enzymes that hydrolyse ATP, releasing ADP and inorganic phosphate. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of LEAK-respiration measured in the absence of ADP, L(n) and subsequent inhibition of respiration by oligomycin, L(Omy).
Reference: LEAK-respiration
MitoPedia topics:
Enzyme
SUITbrowser question: Unspecific ATPase activity
- SUIT protocols can be used to analyze the presence of contaminating ATPases in the sample. The SUIT-024 is focused on answering this question.
- The SUITbrowser was developed for searching for the best SUIT protocols to answer this and other research questions.