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Difference between revisions of "ATPases"

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{{MitoPedia
{{MitoPedia
|abbr=
|abbr=
|description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to production of ADP, which can stimulate respiration. This situation would lead to an overestimations of [[LEAK]] measured in the absence of ADP, L(n).
|description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of [[LEAK-respiration]] measured in the absence of ADP, ''L''(n) and subsequent inhibition of respiration by oligomycin, ''L''(Omy).


|info=ATPase
|info=[[LEAK-respiration]]
}}
}}
{{MitoPedia topics
{{MitoPedia topics
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== SUITbrowser ==
== [[SUITbrowser]] question: Unspecific ATPase activity ==
Work in progress
:::: [[SUIT]] protocols can be used to analyze the presence of contaminating ATPases in the sample. The [[SUIT-024]] is focused on answering this question.
:::: The [https://suitbrowser.oroboros.at/ SUITbrowser] was developed for searching for the best SUIT protocols to answer this and other research questions.





Revision as of 08:52, 3 July 2019


high-resolution terminology - matching measurements at high-resolution


ATPases

Description

ATPases are enzymes that hydrolyse ATP, releasing ADP and inorganic phosphate. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of LEAK-respiration measured in the absence of ADP, L(n) and subsequent inhibition of respiration by oligomycin, L(Omy).


Reference: LEAK-respiration


MitoPedia topics: Enzyme 


SUITbrowser question: Unspecific ATPase activity

SUIT protocols can be used to analyze the presence of contaminating ATPases in the sample. The SUIT-024 is focused on answering this question.
The SUITbrowser was developed for searching for the best SUIT protocols to answer this and other research questions.


List of publications: ATPase