Difference between revisions of "ATPases"
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|description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to production of ADP, which can stimulate respiration. This situation would lead to an overestimation of [[LEAK-respiration]] measured in the absence of ADP, ''L''(n) and subsequent inhibition of respiration by oligomycin, ''L''(Omy). | |description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of [[LEAK-respiration]] measured in the absence of ADP, ''L''(n) and subsequent inhibition of respiration by oligomycin, ''L''(Omy). | ||
|info=[[LEAK-respiration]] | |info=[[LEAK-respiration]] | ||
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== SUITbrowser question: Unspecific ATPase activity == | == [[SUITbrowser]] question: Unspecific ATPase activity == | ||
:::: SUIT protocols can be used to analyze the presence of contaminating ATPases in the sample. | |||
Revision as of 10:36, 2 July 2019
- high-resolution terminology - matching measurements at high-resolution
ATPases
Description
ATPases are enzymes that hydrolyse ATP, releasing ADP and inorganic phosphate. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of LEAK-respiration measured in the absence of ADP, L(n) and subsequent inhibition of respiration by oligomycin, L(Omy).
Reference: LEAK-respiration
MitoPedia topics:
Enzyme
SUITbrowser question: Unspecific ATPase activity
- SUIT protocols can be used to analyze the presence of contaminating ATPases in the sample.
