Difference between revisions of "Air calibration"

Revision as of 11:28, 7 November 2010

Air calibration of the polarographic oxygen sensor is performed routinely on any day before an experiment. Switch on the O2k, select the experimental temperature and gain setting (Oxygraph control window [F7]). Clean the chamber and add experimental medium (at least 2.1 ml for a 2 ml chamber). Then the stopper is fully inserted while the stirrer is on, and excess medium is siphoned off the receptacle of the stopper. Now the stopper is partially opened and positiond with the stopper spacer.

Then the stirred aqeous phase is equilibrated with the oxygen contained in the small air space included in the chamber. Use Graph layout "01 Calibration Exp. Gr3-Temp" or "02 Background Experiment".

After stability is obtained within 20-30 min (the uncorrected slope is close to zero (less than +/- 1 pmol.s^-1.ml^-1), a mark is set on the blue trace of the oxygen signal (R1), and inserted in the calibration window [F5].

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-Air calibration is together with zero calibration one of the two steps of the [[POS calibration]], see [[http://www.oroboros.at/index.php?id=o2k-o2calibration MiPNet12.08]]. It is done at [[open chamber]] after zero( +/- 1 pmol (s ml)) has been achieved. Unlike [[zero calibration]] but like the [[stirrer test]], air calibration should be done before each experiment (and does not take any extra time).
+'''Air calibration''' of the polarographic oxygen sensor is performed routinely on any day before an experiment. Switch on the O2k, select the experimental temperature and gain setting (Oxygraph control window [F7]). Clean the chamber and add experimental medium (at least 2.1 ml for a 2 ml chamber). Then the stopper is fully inserted while the stirrer is on, and excess medium is siphoned off the receptacle of the stopper. Now the stopper is partially opened and positiond with the [[stopper spacer]].
-See [Raw signal]] for the signal to expect during Air calibration.
+Then the stirred aqeous phase is equilibrated with the oxygen contained in the small air space included in the chamber. Use [[Graph layout]] "01 Calibration Exp. Gr3-Temp" or "02 Background Experiment".
+After stability is obtained within 20-30 min (the uncorrected slope is close to zero (less than +/- 1 pmol.s<sup>-1</sup>.ml<sup>-1</sup>), a mark is set on the blue trace of the oxygen signal (R1), and inserted in the calibration window [F5].
+See also: [[Raw signal]], [[Zero calibration]], [[Stirrer test]]
+* [http://www.oroboros.at/index.php?o2k-o2calibration MiPNet12.08]
+* [[Gnaiger_2008_POS]]
 {{#set:Technical service=POS calibration}}