Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Air calibration

From Bioblast

Air calibration of the polarographic oxygen sensor is performed routinely on any day before an experiment. Switch on the O2k, select the experimental temperature and gain setting (Oxygraph control window [F7]). Clean the chamber and add experimental medium (at least 2.1 ml for a 2 ml chamber). Then the stopper is fully inserted while the stirrer is on, and excess medium is siphoned off the receptacle of the stopper. Now the stopper is partially opened and positioned with the stopper spacer.

Then the stirred aqeous phase is equilibrated with the oxygen contained in the small air space included in the chamber. Use Graph layout "01 Calibration Exp. Gr3-Temp" or "02 Background Experiment".

After stability is obtained within 20-30 min (the uncorrected slope is close to zero (less than +/- 1 pmol.s-1.ml-1), a mark is set on the blue trace of the oxygen signal (R1), and inserted in the calibration window [F5].

Perform the oxygen calibration [F5] on-line, such that the calibration parameters are carried over automatically as a default calibration in subsequently opened DatLab files.

Calibration parameters are copied to clipboard, for insertion into the Excel Template "O2k-Calibration-List.xls". This template can be copied from www.oroboros.at.

See also: Raw signal, Zero calibration, Stirrer test, Air calibration for high O2 experiments



Template:Technical service

... more about "Air calibration"
POS calibration +