Amplex UltraRed

high-resolution terminology - matching measurements at high-resolution

Amplex UltraRed

Description

Amplex^® UltraRed (AmR) is used as an extrinsic fluorophore for measurement of hydrogen peroxide production (ROS) by cells or mitochondrial preparations. The reaction of H₂O₂ and AmR is catalyzed by horseradish peroxidase to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm; the fluorescent product according to the supplier is called UltroxRed in the case of Amplex^® UltraRed which has a similar structure to resorufin). The change of emitted fluorescence intensity is directly proportional to the concentration of H₂O₂ added, whereby the H₂O₂ is consumed.

Abbreviation: AmR

Reference: Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004

Amplex Red or Amplex UltraRed in research articles

Citation	Amplex		HRP			pH	Limit of detection
	stock	final	unit definition	stock	final
	mM	µM		U/ml	U/ml
BIOTEK	10	50	pyrogallol	10	0.1	7.4	4 nM (absorption 300 nm)
Life Technologies	10	50		10	0.1	6 to 7.5	<80 nM
Towne 2004		160			0.41	7.5 to 8.5	100 nM
Zhou1997		3 ?			0.3 to 1		50 nM (10 nM optimal)
Mohanty 1997	100	10 to 100			1		18 nM
Komary 2010		1			2.5

Amplex® UltraRed in O2k-FluoRespirometry

O2k-Fluo Smart-Module and O2k-Fluo LED2-Module

In high-resolution respirometry HRR the Amplex® UltraRed method (Mohanty et al 1997) is used with the O2k-FluoRespirometer selecting the Fluorescence-Sensor Green (O2k-Series D-G: O2k-Fluo LED2-Module) and Smart Fluo-Sensor for O2k-Series H - I. Use black stoppers with black cover-slips to exclude disturbances by external light sources. The fluorescence sensors are inserted though the windows of the O2k-Chambers (Hickey et al 2012). The O2k has also been coupled to a fluorescence spectrophotometer with a light guide inserted through the black PEEK stopper (Anderson 2011).

The use of AmR in HRR to simultaneously determine respiration and H₂O₂ flux has been intensively tested in the Oroboros lab (see references).

AmR reacts with H₂O₂ catalysed by horseradish peroxidase.

Application in HRR

Amplex® UltraRed

AmR: Amplex® UltraRed from Life Technologies (former Invitrogen): A36006; 5* 1 mg commercial vials, store at -20°C.

Preparation of 10 mM stock solution (dissolved in DMSO) - see also Manual Amplex® UltraRed by Life Technologies.

Storage solution = stock solution

Prepare a 10 mM storage solution of Amplex® UltraRed reagent (AmR), which will also serve as a stock solution, by adding 340 μL of fresh, high‑quality DMSO (Sigma D8418) to one commercial vial of Amplex® UltraRed reagent (1 mg). Vortex well to dissolve. Protect AmR from light and moisture. The storage solution should then be divided into 20 µL aliquots and stored in the dark in a desiccator, protected from moisture at –20°C for future use. When stored properly, the 10 mM storage solution is stable for 6 months. We have observed that after long-term storage AmR may exert a profound inhibitory effect on cell respiration.

Note: The preparation of the 10 mM storage solution follows the procedure suggested by the manufacturer. The manufacturer states only an approximate molecular weight for the Amplex® UltraRed formulation and does not publish details how the Amplex® UltraRed formulation deviates from the substance 10-acetyl-3,7-dihydroxyphenoxazine (CAS# 119171-73-2), known as Amplex® Red.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration into 2 mL O2k-chamber: 2 µL of 10 mM AmR stock solution, final concentration of 10 µM AmR.

AmR, similar to other chemical probes, may exert an inhibitory effect on mitochondrial and cell respiration. AmR, therefore, should be used at the lowest concentration compatible with the total H₂O₂ production in an experimental run, including calibrations and chemical background conversion of AmR (Makrecka-Kuka et al 2015; Komlodi et al 2018).

You may add 1 µL of the 10 mM stock solution for brief measurements to obtain a final concentration of 5 µM. Optimize the final AmR concentration according to respiration media, sample type and concentration, and experimental protocol. These determine the total AmR consumption by H₂O₂ production during the experiment (check by initial and final H₂O₂ titrations for calibration). The final concentration of AmR becomes diminished during an experiment due to AmR consumption and dilution by titrations in a SUIT protocol. Check for any potential effects of the experimental AmR concentration on respiratory activity of cells or mt-preparations, by titration of AmR after a specific respiratory state has been reached in a control experiment.

Color of AmR and the medium

Amplex UltraRed freshly dissolved should not present any color. The appearance of pink coloring may indicate that the chemical has been compromised.

During the experimental run, the product UltroxRed (or resorufin), generated from the Amplex UltraRed (or Amplex Red) plus H₂O₂ in the reaction catalyzed by horseradish peroxidase, accumulates in the chamber. As this chemical has a pink color, it is normal for the medium to have a pink color at the end of the experiment.

Horseradish peroxidase

Titration into into 2 mL O2k-chamber: 4 µL of HRP stock solution, final concentration 1 U/mL.

Horse radish peroxidase (Sigma-Aldrich P 8250 - 5 KU): Prepare a stock solution with 500 U HRP/mL in MiR05 or MiR05Cr; the solution can be used as storage solution at -20 °C.

Superoxide dismutase

Superoxide dismutase (SOD) is included to generate H₂O₂ from superoxide.

(Sigma-Aldrich S8160-15KU): We recommend to use the enzyme at 5 U/mL, the final volume to be added to the respiratory chamber has thus to be adjusted accordingly.

Experiments can be performed in the absence of SOD.

DTPA

» See: DTPA

Calibration with H₂O₂

Calibration standards of H₂O₂: Commercial solution (Sigma-Aldrich 323381 - 25 mL hydrogen peroxide solution 3 wt.%; stabilized with acetanilide, c. 200 ppm) = 880 mM H₂O₂.

Prepare a HCl stock solution of 10 µM HCl.
H₂O₂ dilution 1 (1:88): add 10 µL of a commercial stock solution of 3 wt. H₂O₂ to 870 µL 10 µM HCl solution, to obtain a 10 mM H₂O₂ solution.
H₂O₂ dilution 2 (1:250):add 4 µL of 10 mM H₂O₂ solution to 996 µL 10 µM HCl solution, to obtain the H₂O₂ stock solution of 40 µM.

The H₂O₂ calibration solution has to be prepared fresh every day, kept away from light exposure, and freezing should be avoided.

Titration into 2 mL O2k-chamber: 5 µL of the 40 µM H₂O₂ stock, step increase of 0.1 µM H₂O₂.

Background H₂O₂ calibration:

A set of H₂O₂ titrations conducted after the addition of respiration medium, (DTPA), HRP and Amplex® UltraRed to the O2k-chamber but before sample addition.
This step is required (1) for the calculation of chemical background fluorescence; see: MiPNet24.10 H2O2 flux analysis and How to analyze H₂O₂ flux in DatLab, and (2) for calculation of the sensitivity of the Amplex® UltraRed assay towards H₂O₂, which, therefore, serves as a quality control step. The sensitivity depends on the respiration medium and on the components of the Amplex® UltraRed assay, and in the same respiration medium, the sensitivity of the AmR assay should be in the same range. For more information: Komlodi 2018 Methods Mol Biol.
Background H₂O₂ calibration has to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex® UltraRed, or H₂O₂, is prepared, or a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
The protocol for background H₂O₂ calibration is provided with DatLab7.4. For further information, see: Instrumental: Browse DL-Protocols and templates

Afterwards we suggest to perform calibrations at the beginning (sample already present), intermittently at various respiratory states, and near the end of an experiment. These calibrations steps are recommended because over the experimental time the sensitivity decreases and chemicals can influence the sensitivity of the AmR assay towards H₂O₂. After the addition of the biological sample, the sensitivity usually decreases owing to the (high) antioxidant capacity of the sample. For further information, see: Komlodi 2018 Methods Mol Biol

Why it is necessary to perform multiple H₂O₂ calibrations in the Amplex® UltraRed (AmR) assay?

The sensitivity to H₂O₂ of the Amplex® UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of (1) experimental time and accumulating UltroxRed (similar to resorufin), (2) changes of the optical properties due to titrations, (3) the radical scavenging capacity of the sample. Therefore, H₂O₂ calibrations are performed before and after sample addition, and after selected titration steps. - »Komlodi 2018 Methods Mol Biol«

The DLPs for AmR SUIT protocols already come with multiple steps of H₂O₂ calibrations implemented.- » SUITbrowser«

DatLab supports automatic H₂O₂ calibration by calculating the calibration parameters by linear regression and graphical display of the calibration regression.

Problem: Here is a DatLab file where I ran the H₂O₂ calibration protocol with 6 H₂O₂ titrations. I drew up 5 uL of H₂O₂ into the syringe for titration into each chamber individually for the first two titrations. For the remaining titrations, I drew up 10 uL of H₂O₂ into the syringe and added 5 uL to one chamber and the rest into the second chamber. For titrations 3 and 4 I added to chamber B first, followed by chamber A. For titrations 5 and 6 I added to chamber A first, followed by chamber B. If sensitivities are measured for each set of titrations based on the manner in which H₂O₂ was added to the chambers, it can be seen that the chamber which is titrated second when 10 µL of H₂O₂ is drawn into the syringe at one time has reduced sensitivity measurements. When fresh H₂O₂ is drawn up for each chamber individually, the sensitivities between chambers are much more comparable. (The numbers shown in the figures are sensitivities expressed as V/µM.)

Answer: It is recommended to fill the syringe with H₂O₂ each time with 5 µL for each O2k-Chamber instead of filling up the syringe with 10 µL and titrate one chamber after the other. Based on tests of Jennifer Norman (US_CA Davis_Roshanravan B) it seems that either the syringe for H₂O₂ titration is not precise filling it up with the maximal volume or H₂O₂ might be degraded by light already in the syringe.

Experimental media for the AmR assay

Media with high antioxidant activity compete with HRP and partially consume H₂O₂ before it can react with AmR to form the active fluorophore UltroxRed (similar to resorufin). This was shown by comparing the resorufin-sensitivity and H₂O₂-sensitivity (Krumschnabel et al 2015; Komlodi et al 2018).

H₂O₂-sensitivity: the change of fluorescence signal per µM H₂O₂ added in calibrations with H₂O₂ titration in media containing HRP and AmR.
Resorufin-sensitivity: the change of fluorescence signal per µM resorufin added in calibrations with resorufin titration in media containing HRP and AmR.

The H₂O₂-sensitivity is much higher in a simple phosphate buffer compared to media with strong antioxidant capacity. In contrast, this is not the case for the resorufin-sensitivity.

H₂O₂ sensitivity in MiR05

We recommend running experiments in MiR05 with a sensitivity >0.5 and <3 at gain 1000 and fluorescence intensity lower than 500 . If the sensitivity is out of this range, please change the light intensity of the Fluo-sensors (in DatLab 7.4: [Oroboros O2k] \ [O2k control], click on the tab [Amperometric, Amp] and change light intensity (Amp polarization voltage [mV]). Do the calibration again by titrating H₂O₂ to check the sensitivity.

The sensitivity values may vary depending on the batch of medium and chemicals used.

The sensitivity value is used to calibrate the amperometric raw signal [V] to H₂O₂ equivalent concentration [µM]. If experiments present different sensitivities, the resulting H₂O₂ fluxes after calibration can still be comparable, as long as the sensitivity values are in the recommended range and the same settings are used for all experiments.

Calculation of background fluorescence slope of MiR05

In the absence of sample, there is a spontaneous increase of the Ultroxred fluorescence signal over time, the extent of which depends on components of the respiration medium (Krumschnabel et al 2015). The sensitivity – the change in fluorescence per unit of H₂O₂ produced in or added to the chamber – depends on the medium and tends to decline over time. Both the background change in fluorescence and the change in assay sensitivity over time need to be corrected for in data analysis, for which DatLab-Analysis templates are available.

The Excel template is ideal for analysis of O₂ and H₂O₂ fluxes measured in MiR05-Kit supplemented with DTPA. The background fluorescence slope of the AmR assay is dependent on the Lot number of the MiR05-Kit, therefore, in each SUIT-###_AmR folder, in the Excel templates for MiR05-Kit (supplemented with DTPA) you can find different analysis templates for each Lot number. The Excel sheets differ from each other in the correction for the background fluorescence slope. You need to use the Excel template which corresponds with the Lot# of MiR05-Kit you use. If you do not add DTPA or use homemade MiR05 instead of the MiR05-Kit, we recommend calculating the background fluorescence slope. The calculation of the background fluorescence slope is also advisable when a new batch of Amplex UltraRed is used or a newly prepared MiR05 is applied. Please follow the steps to measure and calculate background fluorescence slope:

The chemical background flux can be measured as follows: File:AmR background fluorescence slope.DLP
In DatLab 7.4, set the marks to the Amp slope according to the protocol.

a. Go to [Layout] menu and click into ‘O2&Amp´ and select `Standard Layouts/01 Amp Amperometric_Raw signal´.

b. Go to `Marks´ and select ‘Slope uncorrected + all info´. In the new window select `AmR slope [mV/s]´ in `Plot for Marks´.

c. Channel: `Amperometric,Amp´. Leave only this channel selected.

d. Select: `Median´.

e. Sort by: `Time´(default).

f. Then, click on [Copy to clipboard] to copy the selected values.

In the Amp background fluorescence slope.xlsx Excel template (see below): Click on the yellow cell A1 and paste [Ctrl+V] Amp slope from DatLab. If more than one experiment is performed, copy the Amp slope on A32, A63, A94, A126 or A158.
In the "Data" sheet, write the number of the FluoSensors in cells B12. If more than one sensor was tested, write the sensor number in cells B43, B74, B105, B137 or B169. The numbers can be found in the O2k control window (select in ´Oroboros O2k´ menu or press F7), ´Amperometric, Amp´ tab.
Equation required for the correction for the background fluorescence slope using the applied FluoSensor can be found in the figure. The values can be also found in the cells H18 and I18, H48 and I48, H79 and I79, H110 and I110, H142 and I42 or H174 and I174.
It is advisable to measure thebackground fluorescence slope with the same FluoSensors in the same O2k-chambers several times and then calculate the plot of the equation from more experiments. In the Excel template in the ´Summary and equation´ tab, you can copy a° and b° into the table to calculate the equation of plot.
In the SUIT-###_AmR analysis templates, modify the values in the equation for the background fluorescence slope (cells N66, W66, and X66).

The Excel templates are available here: File:AmR background fluorescence slope.xlsx File:AmR background fluorescence slope demo.xlsx

HRP-independent background AmR flux

A study by Miwa et al 2016 (Free Radical Bio Med 90:173) suggests that the HRP-independent artificial background is related to a mitochondrially expressed carboxylesterase which converts Amplex® Red to resorufin at a high rate. The issue can be relatively easily solved by adding a protease inhibitor to various mitochondrial preparations as described here.

For further information see the Discussion page.

AmR assay dilution

How to minimize dilution of the AmR assay upon sample addition

To minimize the dilution of the AmR assay either:

1.) Decrease the titration volume of the sample by using a more concentrated sample solution

2.) Titrate additionally the components of the AmR assay together with the sample

3.) After AmR background calibration, empty and wash the chamber, then add buffer and sample and start titrating the components of the AmR assay into the closed O2k-chamber.

In either case 2 or 3, use media and chemicals from the same batch.

Of note, performing H₂O₂ calibration directly after sample addition allows for calculating the sensitivity to correct the fluorescence signal for sample dilution.

Artefacts

Liver homogenate

Liver homogenate cannot be used with the Amplex® UltraRed assay. - See:Tissue homogenate

Injection artefact

Injection of chemicals (e.g. substrates, uncouplers, inhibitors, H₂O₂) can cause artefacts as observed in the figure:

The marks should always be set on the stable signal avoiding the artefacts area.

NADH and reduced glutathione

Votyakova TV, Reynolds IJ (2014 Arch Biochem Biophys 431:138-44) showed that NADH and reduced glutathione are able to react with AmR in the presence of O₂ and HRP and create a background in the absence of mitochondria. If the mitochondria remain intact, this above-mentioned reaction is negligible. If you add SOD (superoxide dismutase), you can avoid this side-effect of the AmR assay.

Substances incompatible with the Amplex® UltraRed assay

The following substances/ classes of substances are strictly incompatible with the Amplex® Red method for theoretical reasons:

Strongly redox-active substances, e.g. cytochrome c, TMPD/Ascorbate

Inhibitors of horseradish peroxidase, e.g. cyanide, azide; more information: horseradish peroxidase

Catalase and other substances consuming or scavenging H₂O₂. The effect of substances in the medium that slowly consume H₂O₂ is taken into account by the calibration procedure. However, such substances decrease the sensitivity of the method. Note that catalase can be a valuable tool for checking artefacts. See:Avoiding artefacts.

The effect of other substances should be checked by experiments without biological sample, including comparing the sensitivity (result of calibration) before and after injecting the substances.

In preliminary experiments Oroboros Instruments evaluated small amounts (as typically used in SUIT protocols) of the following substances as compatible with the method:

DMSO, ethanol, malate, glutamate, pyruvate (a strong scavanger of H₂O₂), succinate, (ADP + Mg²⁺), (ATP + Mg²⁺), rotenone, FCCP, CCCP, oligomycin, antimycin A, malonate, myxothiazol

Avoiding artefacts

The Amplex® method is based on the H₂O₂ dependent oxidation of AmR to UltroxRed by HRP. Under unfavorable conditions AmR may be oxidized even in the absence of H₂O₂. At a small rate such an oxidation occurs in the presence of HRP even without any sample present. The magnitude of this background signal ("drift") depends, among other things, on the light intensity used and can therefore be minimized by using the suggested or lower light intensity. Components of the sample may however induce a far higher, non H₂O₂ related rate of AmR oxidation. Therefore, especially when applying the method on new types of samples the method should be checked for artifacts. A few approaches are listed here:

Sequential addition of HRP and AmR: This method is particular easy to implement if AmR and HRP have to be added to the chamber already containing the sample anyway: First, inject Amplex® Red and wait a few minutes for flux stabilization. The Amp slope has to stay near zero. Then add HRP. The Amp slope should increase and correspond to the H₂O₂ production. If a significant Amp slope is detected before the addition of HRP this increase in fluorescence is not caused by H₂O₂ production. The experiment can be continued as usual after this test. If the sample is injected routinely into the chamber already containing AmR and HRP the method can not be applied. In this case, it is suggested to change this sequence at least for one experiment.

Addition of catalase: After a H₂O₂ flux (production) is established, a high dose (e.g. 10 µL of a 280,000 U/mL stock solution) of catalase is injected. Catalase competes with HRP for the available H₂O₂. Then the apparent H₂O₂ flux (the Amp slope) should be reduced to near zero as a control for distinguishing an unspecific chemical background slope from H₂O₂ dependent Amplex® UltraRed oxidation.

Note: The experiment cannot be continued afterwards for measurement of hydrogen peroxide production, whereas respiration can be recorded onwards.

Oxygen range

The H₂O₂ production of mitochondria is oxygen dependent Komlodi 2021 BEC AmR-O2. The oxygen concentration can be controlled in the O2k-chamber while running the AmR assay. We recommend running the assay at low O₂ concentration, e.g., between 60 and 30 µM O2. If this is not possible, it is advisable to run all assays in the same [O₂] range, and take marks for different conditions under a similar [O₂] to minimize variability.

Nitrogen or hydrogen injection

To decrease the oxygen concentration in the O2k-chamber using nitrogen or hydrogen see Setting the oxygen concentration.
Check Oxia for fast and safe production of hydrogen (and oxygen).

Permeabilized fibers

Why are permeabilized muscle fibers (pfi) a poor sample type for studying reactive oxygen species (ROS) production?

In experiments with pfi, high oxygen concentrations are needed to avoid oxygen limitation. However, the high oxygen pressures used may artificially increase ROS, including H₂O₂ production, making pfi a less optimal model for ROS production studies. See: Oxygen dependence of pfi

Experimental SOP

H₂O₂ flux analysis and mark setting

Amp slope smoothing in DatLab
MiPNet24.10 H2O2 flux analysis
H2O2 flux analysis video
Mark setting: The black line refers to the raw fluorescence signal [V] of the product of the AmR assay called resorufin (UltroxRed in the case of Amplex® UltraRed), while the green line is the fluorescence slope [mV/s] automatically calculated from the black line.

Excel analysis templates

Excel templates are provided for analysis of the H₂O₂ measurements. It can be found in the upper menu in DatLab in Protocols/SUIT: Browse DL-Protocols and templates and then select your protocol (e.g. SUIT-009/SUIT-009_AmR/SUIT-009_AmR_ce-pce_D019).
The calculations used in the excel analysis template are provided complying with Oroboros transparency policy: [1]
The excel analysis templates are updated for the respective SUIT protocol. The latest versions are available here (last update 2021-09-20):

For SUIT-006 AmR mt D048 protocol, see: File:SUIT-006 AmR mt D048 excel.xlsx and File:SUIT-006 AmR mt D048 demo.xlsx
For SUIT-009 AmR ce-pce D019 protocol, see: File:SUIT-009 AmR ce-pce D019 excel.xlsx and File:SUIT-009 AmR ce-pce D019 demo.xlsx
For SUIT-013 AmR ce D023 protocol, see: File:SUIT-013 AmR ce D023 excel.xlsx and File:SUIT-013 AmR ce D023 demo.xlsx
For SUIT-018 AmR mt D031 protocol, see: File:SUIT-018 AmR mt D031 excel.xlsx and File:SUIT-018 AmR mt D031 demo.xlsx
For SUIT-018 AmR mt D041 protocol, see: File:SUIT-018 AmR mt D041 excel.xlsx and File:SUIT-018 AmR mt D041 demo.xlsx
For SUIT-026 AmR mt D064 protocol, see: File:SUIT-026 AmR mt D064 excel.xlsx and File:SUIT-026 AmR mt D064 demo.xlsx

Manual: MiPNet24.10 H2O2 flux analysis

SUIT protocols

SUIT-006 AmR mt D048: to study the influence of mt-membrane potential on NADH-linked H₂O₂ flux
SUIT-013 AmR ce D023: to detect H₂O₂2 flux in living cells
SUIT-018: to detect NS-linked H₂O₂ flux at different O₂ concentrations
SUIT-026 AmR mt D064: to detect RET-linked H₂O₂ flux

Technical support

Set marks to calibrate the fluorescence signal using Amplex® UltraRed assay

To calibrate the fluorescence signal in molar units [µM], set the marks on the black plot.

» MiPNet20.14 AmplexRed H2O2-production

» Amp calibration - DatLab

References

Mohanty JG, Jaffe JS, Schulman ES, Raible DG (1997) A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. https://doi.org/10.1016/S0022-1759(96)00244-X - »Bioblast link«
Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibers of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). https://doi.org/10.1007/s00360-011-0599-6 - »Bioblast link«
Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. https://doi.org/10.1152/ajpheart.00932.2010 - »Bioblast link«
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 - »Bioblast link«
Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. https://doi.org/10.1007/978-1-4939-7831-1_8 - »Bioblast link«
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. https://doi.org/10.1007/978-1-4939-2257-4_22 - »Bioblast link«
Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004
Komary Z, Tretter L, Adam-Vizi V (2010) Membrane potential-related effect of calcium on reactive oxygen species generation in isolated brain mitochondria. https://doi.org/10.1016/j.bbabio.2010.03.010 - Komary 2010 Biochim Biophys Acta
Mishin V, Gray JP, Heck DE, Laskin DL, Laskin JD (2010) Application of Amplex red/horseradish peroxidase assay to measure hydrogen peroxide production by recombinant microsomal enzymes. https://doi.org/10.1016/j.freeradbiomed.2010.02.030 - Mishin 2010 Free Radical Biol Med
Towne V, Will M, Oswald B, Zhao Q (2004) Complexities in horseradish peroxidase-catalyzed oxidation of dihydroxyphenoxazine derivatives: appropriate ranges for pH values and hydrogen peroxide concentrations in quantitative analysis. https://doi.org/10.1016/j.ab.2004.07.037 - Towne 2004 Anal Biochem
Tretter Laszlo, Adam-Vizi Vera (2012) High Ca2+ load promotes hydrogen peroxide generation via activation of α-glycerophosphate dehydrogenase in brain mitochondria. https://doi.org/10.1016/j.freeradbiomed.2012.09.029 - Tretter 2012 Free Radic Biol Med
Zhou M, Diwu Z, Panchuk-Voloshina N, Haugland RP (1997) A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. https://doi.org/10.1006/abio.1997.2391 - Zhou 1997 Anal Biochem

O2k-Demo experiments

O2k-Publications: H₂O₂

List of publications: Amplex UltraRed - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

	Year	Reference	Organism	Tissue;cell	Stress	Diseases
Li 2024 Sci Rep	2024	Li J, Zhang S, Li C, Zhang X, Shan Y, Zhang Z, Bo H, Zhang Y (2024) Endurance exercise-induced histone methylation modification involved in skeletal muscle fiber type transition and mitochondrial biogenesis. Sci Rep 14:21154. https://doi.org/10.1038/s41598-024-72088-6	Rat	Skeletal muscle
Coulson 2024 J Exp Biol	2024	Coulson SZ, Guglielmo CG, Staples JF (2024) Migration increases mitochondrial oxidative capacity without increasing reactive oxygen species emission in a songbird. J Exp Biol 227:jeb246849. https://doi.org/10.1242/jeb.246849	Birds	Skeletal muscle
Al-Sabri 2024 Sci Rep	2024	Al-Sabri MH, Ammar N, Korzh S, Alsehli AM, Hosseini K, Fredriksson R, Mwinyi J, Williams MJ, Boukhatmi H, Schiöth HB (2024) Fluvastatin-induced myofibrillar damage is associated with elevated ROS, and impaired fatty acid oxidation, and is preceded by mitochondrial morphological changes. https://doi.org/10.1038/s41598-024-53446-w	Drosophila	Skeletal muscle
Cefis 2024 Acta Physiol (Oxf)	2024	Cefis M, Dargegen M, Marcangeli V, Taherkhani S, Dulac M, Leduc-Gaudet JP, Mayaki D, Hussain SNA, Gouspillou G (2024) MFN2 overexpression in skeletal muscles of young and old mice causes a mild hypertrophy without altering mitochondrial respiration and H₂O₂ emission. Acta Physiol (Oxf) [Epub ahead of print]. https://doi.org/10.1111/apha.14119	Mouse	Skeletal muscle		Aging;senescence
Sorby-Adams 2024 Redox Biol	2024	Sorby-Adams A, Prime TA, Miljkovic JL, Prag HA, Krieg T, Murphy MP (2024) A model of mitochondrial superoxide production during ischaemia-reperfusion injury for therapeutic development and mechanistic understanding. Redox Biol 72:103161. https://doi.org/10.1016/j.redox.2024.103161	Rat	Heart	Ischemia-reperfusion Oxidative stress;RONS
Noerregaard 2024 J Physiol	2024	Noerregaard LB, Hansen CC, Wickham KA, Moeller S, Olsen K, Ehlers T, Bangsbo J, Hellsten Y (2024) Exercise training alters skeletal muscle microvascular endothelial cell properties in recent postmenopausal females. J Physiol [Epub ahead of print]. https://doi.org/10.1113/jp286269	Human	Skeletal muscle Endothelial;epithelial;mesothelial cell
Dulac 2024 J Physiol	2024	Dulac M, Hajj-Boutros G, Sonjak V, Faust A, Hussain SNA, Chevalier S, Dionne IJ, Morais JA, Gouspillou G (2024) A multimodal exercise countermeasure prevents the negative impact of head-down tilt bed rest on muscle volume and mitochondrial health in older adults. J Physiol [Epub ahead of print]. https://doi.org/10.1113/jp285897	Human	Skeletal muscle		Aging;senescence
Holwerda 2024 J Cachexia Sarcopenia Muscle	2024	Holwerda AM, Dirks ML, Barbeau PA, Goessens J, Gijsen A, van Loon LJC, Holloway GP (2024) Mitochondrial bioenergetics are not associated with myofibrillar protein synthesis rates. J Cachexia Sarcopenia Muscle [Epub ahead of print]. https://doi.org/10.1002/jcsm.13532	Human Mouse	Skeletal muscle
Dominguez-Lopez 2023 Neuropharmacology	2023	Dominguez-Lopez S, Ahn B, Sataranatarajan K, Ranjit R, Premkumar P, Van Remmen H, Beckstead MJ (2023) Long-term methamphetamine self-administration increases mesolimbic mitochondrial oxygen consumption and decreases striatal glutathione. https://doi.org/10.1016/j.neuropharm.2023.109436	Mouse	Nervous system
Batterson 2023 Physiol Rep	2023	Batterson PM, McGowan EM, Borowik AK, Kinter MT, Miller BF, Newsom SA, Robinson MM (2023) High-fat diet increases electron transfer flavoprotein synthesis and lipid respiration in skeletal muscle during exercise training in female mice. https://doi.org/10.14814/phy2.15840	Mouse	Skeletal muscle
Gautam 2023 Neurobiol Dis	2023	Gautam M, Genç B, Helmold B, Ahrens A, Kuka J, Makrecka-Kuka M, Günay A, Koçak N, Aguilar-Wickings IR, Keefe D, Zheng G, Swaminathan S, Redmon M, Zariwala HA, Özdinler PH (2023) SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function. https://doi.org/10.1016/j.nbd.2023.106022	Rat	Heart Nervous system		Neurodegenerative
Leduc-Gaudet 2023 Nat Commun	2023	Leduc-Gaudet JP, Franco-Romero A, Cefis M, Moamer A, Broering FE, Milan G, Sartori R, Chaffer TJ, Dulac M, Marcangeli V, Mayaki D, Huck L, Shams A, Morais JA, Duchesne E, Lochmuller H, Sandri M, Hussain SNA, Gouspillou G (2023) MYTHO is a novel regulator of skeletal muscle autophagy and integrity. https://doi.org/10.1038/s41467-023-36817-1	Mouse	Skeletal muscle
Salmon 2023 Geroscience	2023	Salmón P, Millet C, Selman C, Monaghan P, Dawson NJ (2023) Tissue-specific reductions in mitochondrial efficiency and increased ROS release rates during ageing in zebra finches, Taeniopygia guttata. https://doi.org/10.1007/s11357-022-00624-1	Birds	Skeletal muscle Liver	Oxidative stress;RONS	Aging;senescence
Devaux 2023 J Comp Physiol B	2023	Devaux JBL, Hedges CP, Birch N, Herbert N, Renshaw GMC, Hickey AJR (2023) Electron transfer and ROS production in brain mitochondria of intertidal and subtidal triplefin fish (Tripterygiidae). https://doi.org/10.1007/s00360-023-01495-4	Fishes	Nervous system	Oxidative stress;RONS
Czyzowska 2023 Redox Biol	2023	Czyżowska A, Brown J, Xu H, Sataranatarajan K, Kinter M, Tyrell VJ, O'Donnell VB, Van Remmen H (2023) Elevated phospholipid hydroperoxide glutathione peroxidase (GPX4) expression modulates oxylipin formation and inhibits age-related skeletal muscle atrophy and weakness. https://doi.org/10.1016/j.redox.2023.102761	Mouse	Skeletal muscle		Aging;senescence
Som 2023 Am J Physiol Cell Physiol	2023	Som R, Fink BD, Yu L, Sivitz WI (2023) Oxaloacetate regulates complex II respiration in brown fat: dependence on UCP1 expression. Am J Physiol Cell Physiol 324:C1236-48. doi: 10.1152/ajpcell.00565.2022	Mouse	Fat	Oxidative stress;RONS	Obesity
Fletcher 2023 Transl Res	2023	Fletcher E, Miserlis D, Sorokolet K, Wilburn D, Bradley C, Papoutsi E, Wilkinson T, Ring A, Ferrer L, Haynatzki G, Smith RS, Bohannon WT, Koutakis P (2023) Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease. https://doi.org/10.1016/j.trsl.2023.05.002	Mouse	Skeletal muscle		Myopathy Obesity
Steffen 2023 J Exp Biol	2023	Steffen JBM, Sokolov EP, Bock C, Sokolova IM (2023) Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas. https://doi.org/10.1242/jeb.246164	Molluscs	Lung;gill	Oxidative stress;RONS Hypoxia
Pharaoh 2023 Geroscience	2023	Pharaoh G, Kamat V, Kannan S, Stuppard RS, Whitson J, Martín-Pérez M, Qian WJ, MacCoss MJ, Villén J, Rabinovitch P, Campbell MD, Sweet IR, Marcinek DJ (2023) The mitochondrially targeted peptide elamipretide (SS-31) improves ADP sensitivity in aged mitochondria by increasing uptake through the adenine nucleotide translocator (ANT). https://doi.org/10.1007/s11357-023-00861-y	Mouse	Skeletal muscle		Aging;senescence
Stouth 2023 Autophagy	2023	Stouth DW, vanLieshout TL, Mikhail AI, Ng SY, Raziee R, Edgett BA, Vasam G, Webb EK, Gilotra KS, Markou M, Pineda HC, Bettencourt-Mora BG, Noor H, Moll Z, Bittner ME, Gurd BJ, Menzies KJ, Ljubicic V (2023) CARM1 drives mitophagy and autophagy flux during fasting-induced skeletal muscle atrophy. https://doi.org/10.1080/15548627.2023.2288528	Mouse	Skeletal muscle
Formiga-Jr 2022 J Vis Exp	2022	Formiga-Jr MA, Camacho-Pereira J (2022) Assessing mitochondrial function in sciatic nerve by high-resolution respirometry. https://doi.org/10.3791/63690	Mouse	Nervous system
Qvit 2022 Pharmaceuticals (Basel)	2022	Qvit N, Lin AJ, Elezaby A, Ostberg NP, Campos JC, Ferreira JCB, Mochly-Rosen D (2022) A selective inhibitor of cardiac troponin I phosphorylation by delta protein kinase C (δPKC) as a treatment for ischemia-reperfusion injury. https://doi.org/10.3390/ph15030271	Rat	Heart	Ischemia-reperfusion
Lopes 2022 Int J Mol Sci	2022	Lopes JA, Collino F, Rodrigues-Ferreira C, Sampaio LDS, Costa-Sarmento G, Wendt CHC, Almeida FP, Miranda KR, Kasai-Brunswick TH, Lindoso RS, Vieyra A (2022) Early effects of extracellular vesicles secreted by adipose tissue mesenchymal cells in renal ischemia followed by reperfusion: mechanisms rely on a decrease in mitochondrial anion superoxide production. https://doi.org/10.3390/ijms23062906	Human	Kidney	Ischemia-reperfusion
Yoval-Sanchez 2022 Redox Biol	2022	Yoval-Sánchez B, Ansari F, James J, Niatsetskaya Z, Sosunov S, Filipenko P, Tikhonova IG, Ten V, Wittig I, Rafikov R, Galkin A (2022) Redox-dependent loss of flavin by mitochondria complex I is different in brain and heart. https://doi.org/10.1016/j.redox.2022.102258	Mouse	Heart Nervous system	Ischemia-reperfusion
Loughland 2022 J Exp Biol	2022	Loughland I, Lau GY, Jolly J, Seebacher F (2022) Rates of warming impact oxidative stress in zebrafish (Danio rerio).	Zebrafish	Skeletal muscle	Oxidative stress;RONS
Starr 2022 Curr Res Physiol	2022	Starr VJ, Dzialowski EM (2022) Developing chicken cardiac muscle mitochondria are resistant to variations in incubation oxygen levels. https://doi.org/10.1016/j.crphys.2022.03.001	Chicken	Heart	Hypoxia
Salyers 2022 JVS Vasc Sci	2022	Salyers ZR, Mariani V, Balestrieri N, Kumar RA, Vugman NA, Thome T, Villani KR, Berceli SA, Scali ST, Vasilakos G, Ryan TE (2022) S100A8 and S100A9 are elevated in chronically threatened ischemic limb muscle and induce ischemic mitochondrial pathology in mice. https://doi.org/10.1016/j.jvssci.2022.03.003	Human Mouse	Skeletal muscle		Cardiovascular
Munro 2022 Mitochondrion	2022	Munro D, Rodríguez E, Blier PU (2022) The longest-lived metazoan, Arctica islandica, exhibits high mitochondrial H₂O₂ removal capacities. https://doi.org/10.1016/j.mito.2022.11.005	Molluscs	Lung;gill Endothelial;epithelial;mesothelial cell	Oxidative stress;RONS	Aging;senescence
Brown 2022 Redox Biol	2022	Brown JL, Peelor FF 3rd, Georgescu C, Wren JD, Kinter M, Tyrrell VJ, O'Donnell VB, Miller BF, Van Remmen H (2022) Lipid hydroperoxides and oxylipins are mediators of denervation induced muscle atrophy. https://doi.org/10.1016/j.redox.2022.102518	Mouse	Skeletal muscle		Other
Xu 2022 Sci Adv	2022	Xu H, Ahn B, Van Remmen H (2022) Impact of aging and oxidative stress on specific components of excitation contraction coupling in regulating force generation. https://doi.org/10.1126/sciadv.add7377	Mouse	Skeletal muscle	Oxidative stress;RONS	Aging;senescence
Sarabhai 2022 Diabetologia	2022	Sarabhai T, Mastrototaro L, Kahl S, Bönhof GJ, Jonuscheit M, Bobrov P, Katsuyama H, Guthoff R, Wolkersdorfer M, Herder C, Meuth SG, Dreyer S, Roden M (2022) Hyperbaric oxygen rapidly improves tissue-specific insulin sensitivity and mitochondrial capacity in humans with type 2 diabetes: a randomised placebo-controlled crossover trial. https://doi.org/10.1007/s00125-022-05797-0	Human	Skeletal muscle Fat		Diabetes
Cruz-Gregorio 2022 Antioxidants (Basel)	2022	Cruz-Gregorio A, Aranda-Rivera AK, Aparicio-Trejo OE, Medina-Campos ON, Sciutto E, Fragoso G, Pedraza-Chaverri J (2022) GK-1 Induces oxidative stress, mitochondrial dysfunction, decreased membrane potential, and impaired autophagy flux in a mouse model of breast cancer. https://doi.org/10.3390/antiox12010056	Mouse	Endothelial;epithelial;mesothelial cell		Cancer
Hansen 2022 Free Radic Biol Med	2022	Hansen C, Møller S, Ehlers T, Wickham KA, Bangsbo J, Gliemann L, Hellsten Y (2022) Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training. https://doi.org/10.1016/j.freeradbiomed.2021.12.265	Human	Endothelial;epithelial;mesothelial cell
Belosludtseva 2022 Membranes (Basel)	2022	Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr ²⁺-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria. https://doi.org/10.3390/membranes12070667	Rat	Liver
Pileggi 2022 EBioMedicine	2022	Pileggi CA, Blondin DP, Hooks BG, Parmar G, Alecu I, Patten DA, Cuillerier A, O'Dwyer C, Thrush AB, Fullerton MD, Bennett SAL, Doucet É, Haman F, Cuperlovic-Culf M, McPherson R, Dent RRM, Harper ME (2022) Exercise training enhances muscle mitochondrial metabolism in diet-resistant obesity. https://doi.org/10.1016/j.ebiom.2022.104192	Human	Skeletal muscle		Obesity
Smith 2022 J Pineal Res	2022	Smith KLM, Swiderska A, Lock MC, Graham L, Iswari W, Choudhary T, Thomas D, Kowash HM, Desforges M, Cottrell EC, Trafford AW, Giussani DA, Galli GLJ (2022) Chronic developmental hypoxia alters mitochondrial oxidative capacity and reactive oxygen species production in the fetal rat heart in a sex-dependent manner. https://doi.org/10.1111/jpi.12821	Rat	Heart	Hypoxia
VanLieshout 2022 Mol Metab	2022	vanLieshout TL, Stouth DW, Hartel NG, Vasam G, Ng SY, Webb EK, Rebalka IA, Mikhail AI, Graham NA, Menzies KJ, Hawke TJ, Ljubicic V (2022) The CARM1 transcriptome and arginine methylproteome mediate skeletal muscle integrative biology. https://doi.org/10.1016/j.molmet.2022.101555	Mouse	Skeletal muscle
McKenna 2022 J Appl Physiol (1985)	2022	McKenna CF, Salvador AF, Keeble AR, Khan NA, De Lisio M, Konopka AR, Paluska SA, Burd NA (2022) Muscle strength after resistance training correlates to mediators of muscle mass and mitochondrial respiration in middle-aged adults. https://doi.org/10.1152/japplphysiol.00186.2022	Human	Skeletal muscle
Riguet 2021 Nat Commun	2021	Riguet N, Mahul-Mellier AL, Maharjan N, Burtscher J, Croisier M, Knott G, Hastings J, Patin A, Reiterer V, Farhan H, Nasarov S, Lashuel HA (2021) Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties. Nat Commun 12:6579.	Human	HEK		Neurodegenerative
Christiansen 2021 Sci Rep	2021	Christiansen LB, Dohlmann TL, Ludvigsen TP, Parfieniuk E, Ciborowski M, Szczerbinski L, Kretowski A, Desler C, Tiano L, Orlando P, Martinussen T, Olsen LH, Larsen S (2021) Atorvastatin impairs liver mitochondrial function in obese Göttingen Minipigs but heart and skeletal muscle are not affected. Sci Rep 11:2167.	Pig	Heart Skeletal muscle Liver
Yardeni 2021 Proc Natl Acad Sci U S A	2021	Yardeni T, Cristancho AG, McCoy AJ, Schaefer PM, McManus MJ, Marsh ED, Wallace DC (2021) An mtDNA mutant mouse demonstrates that mitochondrial deficiency can result in autism endophenotypes. Proc Natl Acad Sci U S A 118:e2021429118.	Mouse	Nervous system		Autism
Furihata 2021 Commun Biol	2021	Furihata T, Takada S, Kakutani N, Maekawa S, Tsuda M, Matsumoto J, Mizushima W, Fukushima A, Yokota T, Enzan N, Matsushima S, Handa H, Fumoto Y, Nio-Kobayashi J, Iwanaga T, Tanaka S, Tsutsui H, Sabe H, Kinugawa S (2021) Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Commun Biol 4:138.	Mouse	Heart		Aging;senescence Cardiovascular
Morgan 2021 Resuscitation	2021	Morgan RW, Sutton RM, Himebauch AS, Roberts AL, Landis WP, Lin Y, Starr J, Ranganathan A, Delso N, Mavroudis CD, Volk L, Slovis J, Marquez AM, Nadkarni VM, Hefti M, Berg RA, Kilbaugh TJ (2021) A randomized and blinded trial of inhaled nitric oxide in a piglet model of pediatric cardiopulmonary resuscitation. Resuscitation 162:274-83 .	Pig	Nervous system		Cardiovascular
Cheng 2021 Comp Biochem Physiol B Biochem Mol Biol	2021	Cheng H, Munro D, Huynh K, Pamenter ME (2021) Naked mole-rat skeletal muscle mitochondria exhibit minimal functional plasticity in acute or chronic hypoxia. Comp Biochem Physiol B Biochem Mol Biol 255:110596.	Other mammals	Skeletal muscle	Hypoxia Oxidative stress;RONS
Vandenberg 2021 Neurochem Int	2021	Vandenberg GG, Dawson NJ, Head A, Scott GR, Scott AL (2021) Astrocyte-mediated disruption of ROS homeostasis in Fragile X mouse model. Neurochem Int 146:105036.	Mouse	Nervous system		Autism
Hansen 2021 Physiol Rep	2021	Hansen C, Olsen K, Pilegaard H, Bangsbo J, Gliemann L, Hellsten Y (2021) High metabolic substrate load induces mitochondrial dysfunction in rat skeletal muscle microvascular endothelial cells. Physiol Rep 9:e14855.	Rat	Skeletal muscle Endothelial;epithelial;mesothelial cell
Flockhart 2021 Cell Metab	2021	Flockhart M, Nilsson LC, Tais S, Ekblom B, Apro W, Larsen FJ (2021) Excessive exercise training causes mitochondrial functional impairment and decreases glucose tolerance in healthy volunteers. Cell Metab 33:957-70.	Human	Skeletal muscle
Marrocco 2021 J Immunol	2021	Marrocco A, Frawley K, Pearce LL, Peterson J, O'Brien JP, Mullett SJ, Wendell SG, St Croix CM, Mischler SE, Ortiz LA (2021) Metabolic adaptation of macrophages as mechanism of defense against crystalline silica. J Immunol 207:1627-40.	Mouse	Macrophage-derived		Other
Apostolopoulou 2021 Sci Adv	2021	Apostolopoulou M, Mastrototaro L, Hartwig S, Pesta D, Straßburger K, de Filippo E, Jelenik T, Karusheva Y, Gancheva S, Markgraf D, Herder C, Nair KS, Reichert AS, Lehr S, Müssig K, Al-Hasani H, Szendroedi J, Roden M (2021) Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males. https://doi.org/10.1126/sciadv.abi9551	Human	Skeletal muscle		Diabetes
Dall 2021 J Biol Chem	2021	Dall M, Hassing AS, Niu L, Nielsen TS, Ingerslev LR, Sulek K, Trammell SAJ, Gillum MP, Barrès R, Larsen S, Poulsen SS, Mann M, Ørskov C, Treebak JT (2021) Hepatocyte-specific perturbation of NAD⁺ biosynthetic pathways in mice induces reversible nonalcoholic steatohepatitis-like phenotypes. J Biol Chem 297:101388.	Mouse	Liver		Other
Theall 2021 Physiol Rep	2021	Theall B, Stampley J, Cho E, Granger J, Johannsen NM, Irving BA, Spielmann G (2021) Impact of acute exercise on peripheral blood mononuclear cells nutrient sensing and mitochondrial oxidative capacity in healthy young adults. Physiol Rep 9:e15147.	Human	Blood cells
Komlodi 2021 BEC AmR-O2	2021	Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004	Saccharomyces cerevisiae	Other cell lines	Oxidative stress;RONS Hypoxia
Sarabhai 2021 Diabetologia	2021	Sarabhai T, Koliaki C, Mastrototaro L, Kahl S, Pesta D, Apostolopoulou M, Wolkersdorfer M, Bönner AC, Bobrov P, Markgraf DF, Herder C, Roden M (2021) Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle. Diabetologia 65:301-14.	Human	Skeletal muscle		Diabetes
Jasz 2021 J Cell Mol Med	2021	Jász DK, Szilágyi ÁL, Tuboly E, Baráth B, Márton AR, Varga P, Varga G, Érces D, Mohácsi Á, Szabó A, Bozó R, Gömöri K, Görbe A, Boros M, Hartmann P (2021) Reduction in hypoxia-reoxygenation-induced myocardial mitochondrial damage with exogenous methane. https://doi.org/10.1111/jcmm.16498	Rat	Heart	Ischemia-reperfusion
Newsom 2021 Med Sci Sports Exerc	2021	Newsom SA, Stierwalt HD, Ehrlicher SE, Robinson MM (2021) Substrate-specific respiration of isolated skeletal muscle mitochondria after 1 h of moderate cycling in sedentary adults. https://doi.org/10.1249/mss.0000000000002615	Human	Skeletal muscle
Pharaoh 2021 JCSM Rapid Commun	2021	Pharaoh G, Brown J, Ranjit R, Ungvari Z, Van Remmen H (2021) Reduced adenosine diphosphate sensitivity in skeletal muscle mitochondria increases reactive oxygen species production in mouse models of aging and oxidative stress but not denervation. https://doi.org/10.1002/rco2.29	Mouse	Skeletal muscle
Falcao-Tebas 2020 J Physiol	2020	Falcão-Tebas F, Marin EC, Kuang J, Bishop DJ, McConell GK (2020) Maternal exercise attenuates the lower skeletal muscle glucose uptake and insulin secretion caused by paternal obesity in female adult rat offspring. J Physiol 598:4251-70.	Rat	Skeletal muscle		Obesity
Smith 2020 J Biol Chem	2020	Smith CD, Schmidt CA, Lin CT, Fisher-Wellman KH, Neufer PD (2020) Flux through mitochondrial redox circuits linked to nicotinamide nucleotide transhydrogenase generates counterbalance changes in energy expenditure. J Biol Chem 295:16207-16.	Mouse	Skeletal muscle	Oxidative stress;RONS
Pham 2020 Eur J Appl Physiol	2020	Pham T, MacRae CL, Broome SC, D'souza RF, Narang R, Wang HW, Mori TA, Hickey AJR, Mitchell CJ, Merry TL (2020) MitoQ and CoQ10 supplementation mildly suppresses skeletal muscle mitochondrial hydrogen peroxide levels without impacting mitochondrial function in middle-aged men. Eur J Appl Physiol 120:1657-69.	Human	Skeletal muscle	Oxidative stress;RONS
Dulac 2020 J Physiol	2020	Dulac M, Leduc-Gaudet JP, Reynaud O, Ayoub MB, Guérin A, Finkelchtein M, Hussain SN, Gouspillou G (2020) Drp1 knockdown induces severe muscle atrophy and remodelling, mitochondrial dysfunction, autophagy impairment and denervation . J Physiol 598:3691-710.	Mouse	Skeletal muscle
Le 2020 J Biol Chem	2020	Le CH, Benage LG, Specht KS, Li Puma LC, Mulligan CM, Heuberger AL, Prenni JE, Claypool SM, Chatfield KC, Sparagna GC, Chicco AJ (2020) Tafazzin deficiency impairs CoA-dependent oxidative metabolism in cardiac mitochondria. J Biol Chem 295:12485-97.	Human	Heart		Aging;senescence
Davidson 2020 Circ Res	2020	Davidson MT, Grimsrud P, Lai L, Draper J, Fisher-Wellman KH, Narowski TM, Koves TR, Kelly DP, Muoio DM (2020) Extreme acetylation of the cardiac mitochondrial proteome does not promote heart failure. Circ Res 127:1094-108.	Mouse	Heart
Hernansanz-Agustin 2020 Nature	2020	Hernansanz-Agustín P, Choya-Foces C, Carregal-Romero S, Ramos E, Oliva T, Villa-Piña T, Moreno L, Izquierdo-Álvarez A, Cabrera-García JD, Cortés A, Lechuga-Vieco AV, Jadiya P, Navarro E, Parada E, Palomino-Antolín A, Tello D, Acín-Pérez R, Rodríguez-Aguilera JC, Navas P, Cogolludo Á, López-Montero I, Martínez-Del-Pozo Á, Egea J, López MG, Elrod JW, Ruíz-Cabello J, Bogdanova A, Enríquez JA, Martínez-Ruiz A (2020) Na⁺ controls hypoxic signalling by the mitochondrial respiratory chain. Nature 586:287-91.	Rat	Heart	Oxidative stress;RONS Hypoxia
Brunetta 2020 J Physiol	2020	Brunetta HS, Politis-Barber V, Petrick HL, Dennis KMJH, Kirsh AJ, Barbeau PA, Nunes EA, Holloway GP (2020) Nitrate attenuates HFD-induced glucose intolerance in association with reduced epididymal adipose tissue inflammation and mitochondrial ROS emission. J Physiol 598:3357-71.	Mouse	Fat		Obesity
Marrocco 2020 Thesis	2020	Marrocco A (2020) Alterations of CCSP expression and macrophages metabolism in the development of silica-induced pulmonary inflammation and fibrosis. PhD Thesis 108.	Mouse	Macrophage-derived		Other
Avila-Rojas 2020 Food Chem Toxicol	2020	Avila-Rojas SH, Aparicio-Trejo OE, Briones-Herrera A, Medina-Campos ON, Reyes-Fermín LM, Martínez-Klimova E, León-Contreras JC, Hernández-Pando R, Tapia E, Pedraza-Chaverri J (2020) Alterations in mitochondrial homeostasis in a potassium dichromate model of acute kidney injury and their mitigation by curcumin. Food Chem Toxicol 145:111774.	Rat	Kidney
Chausse 2020 Brain Behav Immun	2020	Chausse B, Lewen A, Poschet G, Kann O (2020) Selective inhibition of mitochondrial respiratory complexes controls the transition of microglia into a neurotoxic phenotype in situ. Brain Behav Immun 88:802-14.	Mouse	Nervous system
Buch 2020 FASEB J	2020	Buch BT, Halling JF, Ringholm S, Gudiksen A, Kjøbsted R, Olsen MA, Wojtaszewski JFP, Pilegaard H (2020) Colchicine treatment impairs skeletal muscle mitochondrial function and insulin sensitivity in an age-specific manner. FASEB J 34:8653-70.	Mouse	Skeletal muscle		Aging;senescence
Aparicio-Trejo 2020 Free Radic Biol Med	2020	Aparicio-Trejo OE, Avila-Rojas SH, Tapia E, Rojas-Morales P, León-Contreras JC, Martínez-Klimova E, Hernández-Pando R, Sánchez-Lozada LG, Pedraza-Chaverri J (2020) Chronic impairment of mitochondrial bioenergetics and β-oxidation promotes experimental AKI-to-CKD transition induced by folic acid. Free Radic Biol Med 154:18-32.	Rat	Kidney		Other
Zussman 2020 J Autoimmun	2020	Zussman R, Xu LY, Damani T, Groom KM, Chen Q, Seers B, Viall CA, Chamley LW, Hickey A (2020) Antiphospholipid antibodies can specifically target placental mitochondria and induce ROS production. J Autoimmun 111:102437.	Human	Genital	Oxidative stress;RONS
Fuller 2020 J Appl Physiol (1985)	2020	Fuller KNZ, McCoin CS, Allen J, Bell-Glenn S, Koestler DC, Dorn Ii GW, Thyfault JP (2020) Sex and BNIP3 genotype, rather than acute lipid injection, modulate hepatic mitochondrial function and steatosis risk in mice. J Appl Physiol (1985) 128:1251-61.	Mouse	Liver	Oxidative stress;RONS
Li Puma 2020 Am J Physiol Regul Integr Comp Physiol	2020	Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:972-80.	Mouse	Heart Skeletal muscle	Oxidative stress;RONS
Purhonen 2020 Nat Commun	2020	Purhonen J, Grigorjev V, Ekiert R, Aho N, Rajendran J, Pietras R, Truvé K, Wikström M, Sharma V, Osyczka A, Fellman V, Kallijärvi J (2020) A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice. Nat Commun 11:322.	Mouse	Liver Kidney	Mitochondrial disease
Duong 2020 Mitochondrion	2020	Duong QV, Hoffman A, Zhong K, Dessinger MJ, Zhang Y, Bazil JN (2020) Calcium overload decreases net free radical emission in cardiac mitochondria. Mitochondrion 51:126-39.	Guinea pig	Heart	Ischemia-reperfusion Oxidative stress;RONS
Hedges 2020 Biosci Rep	2020	Hedges CP, Pham T, Shetty B, Masson SWC, Hickey AJR, Shepherd PR, Merry TL (2020) Prolonged treatment with a PI3K p110α inhibitor causes sex- and tissue-dependent changes in antioxidant content, but does not affect mitochondrial function. https://doi.org/10.1042/bsr20201128	Mouse	Skeletal muscle Liver
Mendham 2020 Sci Rep	2020	Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785.		Fat		Diabetes Obesity
Pharaoh 2020 Sci Rep	2020	Pharaoh G, Brown JL, Sataranatarajan K, Kneis P, Bian J, Ranjit R, Hadad N, Georgescu C, Rabinovitch P, Ran Q, Wren JD, Freeman W, Kinter M, Richardson A, Van Remmen H (2020) Targeting cPLA₂ derived lipid hydroperoxides as a potential intervention for sarcopenia. Sci Rep 10:13968.	Mouse	Skeletal muscle	Oxidative stress;RONS	Aging;senescence
Hartmann 2020 Free Radic Res	2020	Hartmann DD, Gonçalves DF, Da Rosa PC, Martins RP, Courtes AA, Franco JL, A Soares FA, Puntel GO (2020) A single muscle contusion promotes an immediate alteration in mitochondrial bioenergetics response in skeletal muscle fibres with different metabolism. Free Radic Res 54:137-49.	Rat	Skeletal muscle		Other
Szibor 2020 J Cell Mol Med	2020	Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet M, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48.	Mouse	Heart	Ischemia-reperfusion
Dirks 2020 J Physiol	2020	Dirks ML, Miotto PM, Goossens GH, Senden JM, Petrick HL, van Kranenburg J, van Loon LJC, Holloway GP (2020) Short-term bed rest-induced insulin resistance cannot be explained by increased mitochondrial H₂O₂ emission. J Physiol 598:123-37.	Human	Skeletal muscle	Oxidative stress;RONS
Ehrlicher 2020 FASEB J	2020	Ehrlicher SE, Stierwalt HD, Miller BF, Newsom SA, Robinson MM (2020) Mitochondrial adaptations to exercise do not require Bcl2-mediated autophagy but occur with BNIP3/Parkin activation. FASEB J 34:4602-18.	Mouse	Skeletal muscle
Baxter 2020 Elife	2020	Baxter M, Voronkov M, Poolman T, Galli G, Pinali C, Goosey L, Knight A, Krakowiak K, Maidstone R, Iqbal M, Zi M, Prehar S, Cartwright EJ, Gibbs J, Matthews LC, Adamson AD, Humphreys NE, Rebelo-Guiomar P, Minczuk M, Bechtold DA, Loudon A, Ray D (2020) Cardiac mitochondrial function depends on BUD23 mediated ribosome programming. Elife 9:e50705.	Mouse	Heart
Hafstad 2020 Antioxidants (Basel)	2020	Hafstad AD, Hansen SS, Lund J, Santos CXC, Boardman NT, Shah AM, Aasum E (2020) NADPH oxidase 2 mediates myocardial oxygen wasting in obesity. Antioxidants (Basel) 9:E171.	Mouse	Heart	Oxidative stress;RONS	Obesity
Burtscher 2020 eNeuro	2020	Burtscher J, Copin JC, Sandi C, Lashuel HA (2020) Pronounced α-synuclein pathology in a seeding-based mouse model is not sufficient to induce mitochondrial respiration deficits in the striatum and amygdala. eNeuro 7:ENEURO.0110-20.2020.	Mouse	Nervous system		Parkinson's
Mahul-Mellier 2020 Proc Natl Acad Sci U S A	2020	Mahul-Mellier AL, Burtscher J, Maharjan N, Weerens L, Croisier M, Kuttler F, Leleu M, Knott GW, Lashuel HA (2020) The process of Lewy body formation, rather than simply α-synuclein fibrillization, is one of the major drivers of neurodegeneration. Proc Natl Acad Sci U S A 117:4971-82.	Mouse	Nervous system		Parkinson's
Marquez 2020 J Am Heart Assoc	2020	Marquez AM, Morgan RW, Ko T, Landis WP, Hefti MM, Mavroudis CD, McManus MJ, Karlsson M, Starr J, Roberts AL, Lin Y, Nadkarni V, Licht DJ, Berg RA, Sutton RM, Kilbaugh TJ (2020) Oxygen exposure during cardiopulmonary resuscitation is associated with cerebral oxidative injury in a randomized, blinded, controlled, preclinical trial. J Am Heart Assoc 9:015032.	Pig	Nervous system		Cardiovascular
Makrecka-Kuka 2020 Sci Rep	2020	Makrecka-Kuka M, Dimitrijevs P, Domracheva I, Jaudzems K, Dambrova M, Arsenyan P (2020) Fused isoselenazolium salts suppress breast cancer cell growth by dramatic increase in pyruvate-dependent mitochondrial ROS production. https://doi.org/10.1038/s41598-020-78620-8.	Mouse	Endothelial;epithelial;mesothelial cell		Cancer
Charles 2020 Nanomedicine (Lond)	2020	Charles C, Cohen-Erez I, Kazaoka B, Melnikov O, Stein DE, Sensenig R, Rapaport H, Orynbayeva Z (2020) Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes. Nanomedicine (Lond) 15:2917-32.	Human	Endothelial;epithelial;mesothelial cell
Kupats 2020 Oxid Med Cell Longev	2020	Kupats E, Stelfa G, Zvejniece B, Grinberga S, Vavers E, Makrecka-Kuka M, Svalbe B, Zvejniece L, Dambrova M (2020) Mitochondrial-protective effects of R-phenibut after experimental traumatic brain injury. Oxid Med Cell Longev 2020:9364598.	Mouse	Nervous system		Other
Kunz 2020 J Biol Chem	2020	Kunz HE, Dorschner JM, Berent TE, Meyer T, Wang X, Jatoi A, Kumar R, Lanza IR (2020) Methylarginine metabolites are associated with attenuated muscle protein synthesis in cancer-associated muscle wasting. J Biol Chem 295:17441-59.	Mouse	Skeletal muscle		Cancer
Olsen 2020 J Physiol	2020	Olsen LN, Hoier B, Hansen CV, Leinum M, Carter HH, Jorgensen TS, Bangsbo J, Hellsten Y (2020) Angiogenic potential is reduced in skeletal muscle of aged women. J Physiol 598:5149-64.	Human	Endothelial;epithelial;mesothelial cell		Aging;senescence
Silva-Rodrigues 2020 Free Radic Biol Med	2020	Silva-Rodrigues T, De-Souza-Ferreira E, Machado CM, Cabral-Braga B, Rodrigues-Ferreira C, Galina A (2020) Hyperglycemia in a Type 1 Diabetes Mellitus model causes a shift in mitochondria coupled-glucose phosphorylation and redox metabolism in rat brain. Free Radic Biol Med 160:796-806.	Rat	Nervous system	Oxidative stress;RONS	Diabetes
Bundgaard 2020 J Exp Biol	2020	Bundgaard A, Qvortrup K, Rasmussen LJ, Fago A (2020) Turtles maintain mitochondrial integrity but reduce mitochondrial respiratory capacity in the heart after cold acclimation and anoxia. J Exp Biol 222:jeb200410.	Amphibians	Heart
Abid 2020 FASEB J	2020	Abid H, Ryan ZC, Delmotte P, Sieck GC, Lanza IR (2020) Extramyocellular interleukin-6 influences skeletal muscle mitochondrial physiology through canonical JAK/STAT signaling pathways. FASEB J 34:14458-72.	Mouse	Skeletal muscle	Oxidative stress;RONS
Souza da Silva 2020 Mol Neurobiol	2020	Souza da Silva J, Nonose Y, Rohden F, Lukasewicz Ferreira PC, Fontella FU, Rocha A, Wigner Brochier A, Vieira Apel R, de Lima TM, Seminotti B, Amaral AU, Galina A, Souza DO (2020) Guanosine neuroprotection of presynaptic mitochondrial calcium homeostasis in a mouse study with amyloid-β oligomers. Mol Neurobiol 57:4790-809.	Mouse	Nervous system		Alzheimer's
Scheiber 2019 Exp Mol Med	2019	Scheiber D, Jelenik T, Zweck E, Horn P, Schultheiss HP, Lassner D, Boeken U, Saeed D, Kelm M, Roden M, Westenfeld R, Szendroedi J (2019) High-resolution respirometry in human endomyocardial biopsies shows reduced ventricular oxidative capacity related to heart failure. Exp Mol Med 51:16.	Human	Heart		Cardiovascular
Laouafa 2019 Acta Physiol (Oxf)	2019	Laouafa S, Roussel D, Marcouiller F, Soliz J, Gozal D, Bairam A, Joseph V (2019) Roles of oestradiol receptor alpha and beta against hypertension and brain mitochondrial dysfunction under intermittent hypoxia in female rats. Acta Physiol (Oxf) 226:e13255.	Rat	Nervous system	Hypoxia
Monteiro 2019 J Bioenerg Biomembr	2019	Monteiro J, Assis-de-Lemos G, de-Souza-Ferreira E, Marques AM, Neves GA, Silveira MS, Galina A (2019) Energization by multiple substrates and calcium challenge reveal dysfunctions in brain mitochondria in a model related to acute psychosis. J Bioenerg Biomembr 52:1-15.	Mouse	Nervous system	Oxidative stress;RONS	Other
Miotto 2019 FASEB J	2019	Miotto PM, McGlory C, Bahniwal R, Kamal M, Phillips SM, Holloway GP (2019) Supplementation with dietary ω-3 mitigates immobilization-induced reductions in skeletal muscle mitochondrial respiration in young women. FASEB J 33:8232-40.	Human	Skeletal muscle
Perry 2019 J Mol Cell Cardiol	2019	Perry JB, Davis GN, Allen ME, Makrecka-Kuka M, Dambrova M, Grange RW, Shaikh SR, Brown DA (2019) Cardioprotective effects of idebenone do not involve ROS scavenging: Evidence for mitochondrial complex I bypass in ischemia/reperfusion injury. J Mol Cell Cardiol 135:160-171.	Rat	Heart	Ischemia-reperfusion
Lefranc 2019 Hypertension	2019	Lefranc C, Friederich-Persson M, Braud L, Palacios-Ramirez R, Karlsson S, Boujardine N, Motterlini R, Jaisser F, Nguyen Dinh Cat A (2019) MR (mineralocorticoid receptor) induces adipose tissue senescence and mitochondrial dysfunction leading to vascular dysfunction in obesity. Hypertension 73:458-68.	Mouse	Fat		Aging;senescence Obesity
Robinson 2019 Am J Physiol Cell Physiol	2019	Robinson MM, Sather BK, Burney ER, Ehrlicher SE, Stierwalt HD, Franco MC, Newsom SA (2019) Robust intrinsic differences in mitochondrial respiration and H₂O₂ emission between L6 and C2C12 cells. Am J Physiol Cell Physiol 317:C339-C347.	Mouse Rat	Skeletal muscle
Hedges 2019 Comp Biochem Physiol A Mol Integr Physiol	2019	Hedges CP, Wilkinson RT, Devaux JBL, Hickey AJR (2019) Hymenoptera flight muscle mitochondrial function: Increasing metabolic power increases oxidative stress. Comp Biochem Physiol A Mol Integr Physiol 230:115-21.	Other invertebrates	Skeletal muscle	Oxidative stress;RONS
Hernansanz-Agustin 2019 bioRxiv	2019	Hernansanz-Agustín P, Choya-Foces C, Carregal-Romero S, Ramos E, Oliva T, Villa-Piña T, Moreno L, Izquierdo-Álvarez A, JCabrera-García JD, Cortés A, Lechuga-Vieco AV, Jadiya P, Navarro E, Parada E, Palomino-Antolín A, Tello D, Acín-Pérez R, Rodríguez-Aguilera JC, Navas P, Cogolludo A, López-Montero I, Martínez-del-Pozo A, Egea J, López MG, Elrod JW, Ruiz-Cabello J, Bogdanova A, Enríquez JA, Martínez-Ruiz A (2019) Mitochondrial Na⁺ import controls oxidative phosphorylation and hypoxic redox signalling. bioRxiv doi: https://doi.org/10.1101/385690.	Rat Bovines	Heart	Hypoxia
Seo 2019 Exp Neurol	2019	Seo JH, Park HS, Park SS, Kim CJ, Kim DH, Kim TW (2019) Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder. Exp Neurol 322:113043.	Rat	Nervous system		Other
Holsgrove 2019 Thesis	2019	Holsgrove A (2019) The effect of temperature on cardiac energetics in the rainbow trout, Onchorhynchus mykiss. Phd Thesis 166.	Fishes	Heart
Ruegsegger 2019 FASEB J	2019	Ruegsegger GN, Manjunatha S, Summer P, Gopala S, Zabeilski P, Dasari S, Vanderboom PM, Lanza IR, Klaus KA, Nair KS (2019) Insulin deficiency and intranasal insulin alter brain mitochondrial function: a potential factor for dementia in diabetes. FASEB J 33:4458-72.	Mouse	Nervous system	Oxidative stress;RONS	Diabetes
Halling 2019 Am J Physiol Endocrinol Metab	2019	Halling JF, Jessen H, Nøhr-Meldgaard J, Thiellesen Buch B, Masselkhi Christensen N, Gudiksen A, Ringholm S, Neufer PD, Prats C, Pilegaard H (2019) PGC-1α regulates mitochondrial properties beyond biogenesis with aging and exercise training. Am J Physiol Endocrinol Metab 317:E513-E525.	Mouse	Skeletal muscle		Aging;senescence
Szibor 2019 Biochim Biophys Acta Bioenerg	2019	Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137.	Mouse	Heart
McBride 2019 Arch Biochem Biophys	2019	McBride S, Wei-LaPierre L, McMurray F, MacFarlane M, Qiu X, Patten DA, Dirksen RT, Harper ME (2019) Skeletal muscle mitoflashes, pH, and the role of uncoupling protein-3. Arch Biochem Biophys 663:239-48.	Mouse	Skeletal muscle	Oxidative stress;RONS
Ahn 2019 J Cachexia Sarcopenia Muscle	2019	Ahn B, Ranjit R, Premkumar P, Pharaoh G, Piekarz KM, Matsuzaki S, Claflin DR, Riddle K, Judge J, Bhaskaran S, Satara Natarajan K, Barboza E, Wronowski B, Kinter M, Humphries KM, Griffin TM, Freeman WM, Richardson A, Brooks SV, Van Remmen H (2019) Mitochondrial oxidative stress impairs contractile function but paradoxically increases muscle mass via fiber branching. J Cachexia Sarcopenia Muscle 10:411-28.	Mouse	Skeletal muscle	Oxidative stress;RONS
Shirakawa 2019 Sci Rep	2019	Shirakawa R, Yokota T, Nakajima T, Takada S, Yamane M, Furihata T, Maekawa S, Nambu H, Katayama T, Fukushima A, Saito A, Ishimori N, Dela F, Kinugawa S, Anzai T (2019) Mitochondrial reactive oxygen species generation in blood cells is associated with disease severity and exercise intolerance in heart failure patients. Sci Rep 9:14709.	Human	Blood cells	Oxidative stress;RONS	Cardiovascular
Spinazzi 2019 Proc Natl Acad Sci U S A	2019	Spinazzi M, Radaelli E, Horré K, Arranz AM, Gounko NV, Agostinis P, Maia TM, Impens F, Morais VA, Lopez-Lluch G, Serneels L, Navas P, De Strooper B (2019) PARL deficiency in mouse causes Complex III defects, coenzyme Q depletion, and Leigh-like syndrome. Proc Natl Acad Sci U S A 116:277-86. 10.1073/pnas.1811938116	Mouse	Nervous system		Neurodegenerative
Rajendran 2019 EMBO Mol Med	2019	Rajendran J, Purhonen J, Tegelberg S, Smolander OP, Mörgelin M, Rozman J, Gailus-Durner V, Fuchs H, Hrabe de Angelis M, Auvinen P, Mervaala E, Jacobs HT, Szibor M, Fellman V, Kallijärvi J (2019) Alternative oxidase-mediated respiration prevents lethal mitochondrial cardiomyopathy. EMBO Mol Med 11:e9456.	Mouse	Liver Kidney Heart	Mitochondrial disease
Pharaoh 2019 Mol Neurobiol	2019	Pharaoh G, Owen D, Yeganeh A, Premkumar P, Farley J, Bhaskaran S, Ashpole N, Kinter M, Van Remmen H, Logan S (2019) Disparate central and peripheral effects of circulating IGF-1 deficiency on tissue mitochondrial function. Mol Neurobiol 57:1317-31.	Mouse	Skeletal muscle Nervous system Fat		Aging;senescence
Power 2019 PLoS One	2019	Power AS, Norman R, Jones TLM, Hickey AJ, Ward ML (2019) Mitochondrial function remains impaired in the hypertrophied right ventricle of pulmonary hypertensive rats following short duration metoprolol treatment. PLoS One 14:e0214740.	Rat	Heart		Cardiovascular
Courtes 2019 Biomed Pharmacother	2019	Courtes AA, de Carvalho NR, Gonçalves DF, Hartmann DD, da Rosa PC, Dobrachinski F, Franco JL, de Souza DOG, Soares FAA (2019) Guanosine protects against Ca²⁺-induced mitochondrial dysfunction in rats. Biomed Pharmacother 111:1438-46.	Rat	Liver
Ruegsegger 2019 JCI Insight	2019	Ruegsegger GN, Vanderboom PM, Dasari S, Klaus KA, Kabiraj P, McCarthy CB, Lucchinetti CF, Nair KS (2019) Exercise and metformin counteract altered mitochondrial function in the insulin-resistant brain. JCI Insight 4:130681.	Mouse	Nervous system		Diabetes
Cannon 2019 J Appl Physiol (1985)	2019	Cannon DT, Rodewohl L, Adams V, Breen EC, Bowen TS (2019) Skeletal myofiber VEGF deficiency leads to mitochondrial, structural and contractile alterations in mouse diaphragm. J Appl Physiol (1985) 127:1360-69.	Mouse	Skeletal muscle
Bundgaard 2019 Sci Rep	2019	Bundgaard A, James AM, Gruszczyk AV, Martin J, Murphy MP, Fago A (2019) Metabolic adaptations during extreme anoxia in the turtle heart and their implications for ischemia-reperfusion injury. Sci Rep 9:2850.	Mouse Reptiles	Heart	Ischemia-reperfusion Oxidative stress;RONS
Desquiret-Dumas 2019 Biochim Biophys Acta Mol Basis Dis	2019	Desquiret-Dumas V, Leman G, Wetterwald C, Chupin S, Lebert A, Khiati S, Le Mao M, Geffroy G, Kane MS, Chevrollier A, Goudenege D, Gadras C, Tessier L, Barth M, Leruez S, Amati-Bonneau P, Henrion D, Bonneau D, Procaccio V, Reynier P, Lenaers G, Gueguen N (2019) Warburg-like effect is a hallmark of complex I assembly defects. Biochim Biophys Acta Mol Basis Dis 1865:2475-89.	Human	Fibroblast		Other
Cooper 2019 Exp Physiol	2019	Cooper MA, McCoin C, Pei D, Thyfault JP, Koestler D, Wright DE (2019) Reduced mitochondrial reactive oxygen species production in peripheral nerves of mice fed a ketogenic diet. Exp Physiol 103:1206-12.	Mouse	Nervous system
Stepanova 2019 Antioxid Redox Signal	2019	Stepanova Anna, Sosunov S, Niatsetskaya Z, Konrad Csaba, Starkov AA, Manfredi G, Wittig I, Ten V, Galkin A (2019) Redox-dependent loss of flavin by mitochondrial complex I in brain ischemia/reperfusion injury. Antioxid Redox Signal 31:608-22.	Mouse	Nervous system	Ischemia-reperfusion
Isei 2019 Free Radic Biol Med	2019	Isei MO, Kamunde C (2019) Effects of copper and temperature on heart mitochondrial hydrogen peroxide production. Free Radic Biol Med 147:114-28.	Fishes	Heart	Oxidative stress;RONS
Ederle 2019 J Clin Med	2019	Ederle C, Charles AL, Khayath N, Poirot A, Meyer A, Clere-Jehl R, Andres E, De Blay F, Geny B (2019) Mitochondrial function in peripheral blood mononuclear cells (PBMC) is enhanced, together with increased reactive oxygen species, in severe asthmatic patients in exacerbation. J Clin Med 8:E1613.	Human	Blood cells	Oxidative stress;RONS	Other
McMurray 2019 FASEB J	2019	McMurray F, MacFarlane M, Kim K, Patten DA, Wei-LaPierre L, Fullerton MD, Harper ME (2019) Maternal diet-induced obesity alters muscle mitochondrial function in offspring without changing insulin sensitivity. FASEB J 33:13515-26.	Mouse	Skeletal muscle	Oxidative stress;RONS	Diabetes Obesity
Munro 2019 Aging Cell	2019	Munro D, Baldy C, Pamenter ME, Treberg JR (2019) The exceptional longevity of the naked mole-rat may be explained by mitochondrial antioxidant defenses. Aging Cell 18:e12916.	Mouse Other mammals	Heart Skeletal muscle	Oxidative stress;RONS	Aging;senescence
Axton 2019 J Nutr	2019	Axton ER, Beaver LM, St Mary L, Truong L, Logan CR, Spagnoli S, Prater MC, Keller RM, Garcia-Jaramillo M, Ehrlicher SE, Stierwalt HD, Newsom SA, Robinson MM, Tanguay RL, Stevens JF, Hord NG (2019) Treatment with nitrate, but not nitrite, lowers the oxygen cost of exercise and decreases glycolytic intermediates while increasing fatty acid metabolites in exercised zebrafish. J Nutr 00:1–13.	Zebrafish	Skeletal muscle
Karlsson 2018 J Neurotrauma	2018	Karlsson M, Pukenas B, Chawla S, Ehinger JK, Plyler R, Stolow M, Gabello M, Hugerth M, Elmér E, Hansson MJ, Margulies S, Kilbaugh T (2018) Neuroprotective effects of cyclosporine in a porcine pre-clinical trial of focal traumatic brain injury. J Neurotrauma 36:14-24.	Pig	Nervous system		Other
Treberg 2018 Comp Biochem Physiol B Biochem Mol Biol	2018	Treberg JR, Braun K, Zacharias P, Kroeker K (2018) Multidimensional mitochondrial energetics: Application to the study of electron leak and hydrogen peroxide metabolism. Comp Biochem Physiol B Biochem Mol Biol 224:121-28.	Mouse Rat	Skeletal muscle
Stepanova 2018 J Cereb Blood Flow Metab	2018	Stepanova A, Konrad C, Guerrero-Castillo S, Manfredi G, Vannucci S, Arnold S, Galkin A (2018) Deactivation of mitochondrial complex I after hypoxia-ischemia in the immature brain. J Cereb Blood Flow Metab 39:1790-802.	Rat	Nervous system	Hypoxia Ischemia-reperfusion
Scheiber 2018 J Cardiovasc Transl Res	2018	Scheiber D, Zweck E, Jelenik T, Horn P, Albermann S, Masyuk M, Boeken U, Saeed D, Kelm M Roden M, Szendroedi J, Westenfeld R (2018) Reduced myocardial mitochondrial ROS production in mechanically unloaded hearts. J Cardiovasc Transl Res 12:107-15.	Human	Heart		Cardiovascular
Holland 2018 Cell Death Dis	2018	Holland OJ, Cuffe JSM, Dekker Nitert M, Callaway L, Kwan Cheung KA, Radenkovic F, Perkins AV (2018) Placental mitochondrial adaptations in preeclampsia associated with progression to term delivery. Cell Death Dis 9:1150.	Human	Genital	Ischemia-reperfusion	Other
Kamunde 2018 Free Radic Biol Med	2018	Kamunde C, Sharaf M, MacDonald N (2018) H₂O₂ metabolism in liver and heart mitochondria: Low emitting-high scavenging and high emitting-low scavenging systems. Free Radic Biol Med 124:135-48.	Fishes	Heart Liver	Oxidative stress;RONS
Campbell 2018 Free Radic Biol Med	2018	Campbell MD, Duan J, Samuelson AT, Gaffrey MJ, Wang L, Bammler TK, Moore RJ, White CC, Kavanagh TJ, Voss JG, Szeto HH, Rabinovitch PS, Qian WJ, Marcinek DJ (2018) Improving mitochondrial function with SS-31 reverses age-related redox stress and improves exercise tolerance in aged mice. Free Radic Biol Med 134:268-81.	Mouse	Skeletal muscle	Oxidative stress;RONS	Aging;senescence
Burney 2018 Thesis	2018	Burney E (2018) Characterization of mitochondrial metabolism in L6 rat myoblasts. Bachelor's Thesis p50.	Rat	Other cell lines
Holloway 2018 Cell Rep	2018	Holloway GP, Holwerda AM, Miotto PM, Dirks ML, Verdijk LB, van Loon LJC (2018) Age-associated impairments in mitochondrial ADP sensitivity contribute to redox stress in senescent human skeletal muscle. Cell Rep 22:2837–48.	Human	Skeletal muscle	Oxidative stress;RONS	Aging;senescence
Aparicio-Trejo 2018 Free Radic Biol Med	2018	Aparicio-Trejo OE, Reyes-Fermín LM, Briones-Herrera A, Tapia E, León-Contreras JC, Hernández-Pando R, Sánchez-Lozada LG, Pedraza-Chaverri J (2018) Protective effects of N-acetyl-cysteine in mitochondria bioenergetics, oxidative stress, dynamics and S-glutathionylation alterations in acute kidney damage induced by folic acid. Free Radic Biol Med 130:379-96.	Rat	Kidney	Oxidative stress;RONS	Other
Robb 2018 J Biol Chem	2018	Robb EL, Hall AR, Prime TA, Eaton S, Szibor M, Viscomi C, James AM, Murphy MP (2018) Control of mitochondrial superoxide production by reverse electron transport at complex I. J Biol Chem 293:9869-79. https://doi.org/10.1074/jbc.RA118.003647	Rat	Heart	Oxidative stress;RONS
Stepanova 2018 J Neurochem	2018	Stepanova A, Konrad C, Manfredi G, Springett R, Ten V, Galkin A (2018) The dependence of brain mitochondria reactive oxygen species production on oxygen level is linear, except when inhibited by antimycin A. J Neurochem 148:731-45.	Mouse	Nervous system	Ischemia-reperfusion Oxidative stress;RONS
Souza 2018 Sci Rep	2018	Souza RWA, Alves CRR, Medeiros A, Rolim N, Silva GJJ, Moreira JBN, Alves MN, Wohlwend M, Gebriel M, Hagen L, Sharma A, Koch LG, Britton SL, Slupphaug G, Wisløff U, Brum PC (2018) Differential regulation of cysteine oxidative post-translational modifications in high and low aerobic capacity. Sci Rep 8:17772.	Rat	Heart Skeletal muscle
Schiffer 2018 PLoS One	2018	Schiffer TA, Christensen M, Gustafsson H, Palm F (2018) The effect of inactin on kidney mitochondrial function and production of reactive oxygen species. PLoS One 13:e0207728.	Rat	Kidney	Oxidative stress;RONS
Briones-Herrera 2018 Food Chem Toxicol	2018	Briones-Herrera A, Avila-Rojas SH, Aparicio-Trejo OE, Cristóbal M, León-Contreras JC, Hernández-Pando R, Pinzón E, Pedraza-Chaverri J, Sánchez-Lozada LG, Tapia E (2018) Sulforaphane prevents maleic acid-induced nephropathy by modulating renal hemodynamics, mitochondrial bioenergetics and oxidative stress. Food Chem Toxicol 115:185-97.	Rat	Kidney		Other
Smenes 2018 Interact Cardiovasc Thorac Surg	2018	Smenes BT, Bækkerud FH, Slagsvold KH, Hassel E, Wohlwend M, Pinho M, Høydal M, Wisløff U, Rognmo Ø, Wahba A (2018) Acute exercise is not cardioprotective and may induce apoptotic signalling in heart surgery: a randomized controlled trial. Interact Cardiovasc Thorac Surg 27:95-101.	Human	Heart	Ischemia-reperfusion	Aging;senescence Cardiovascular
Jelenik 2018 Diabetes	2018	Jelenik T, Flögel U, Álvarez-Hernández E, Scheiber D, Zweck E, Ding Z, Rothe M, Mastrototaro L, Kohlhaas V, Kotzka J, Knebel B, Müller-Wieland D, Moellendorf S, Gödecke A, Kelm M, Westenfeld R, Roden M, Szendroedi J (2018) Insulin resistance and vulnerability to cardiac ischemia. Diabetes 67:2695-702.	Human Mouse	Heart	Ischemia-reperfusion	Cardiovascular Diabetes
Komlodi 2018 Methods Mol Biol	2018	Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.	Human Mouse	Skeletal muscle HEK
Valentine 2018 J Gerontol A Biol Sci Med Sci	2018	Valentine JM, Li ME, Shoelson SE, Zhang N, Reddick RL, Musi N (2018) NFκB regulates muscle development and mitochondrial function. J Gerontol A Biol Sci Med Sci 75:647-53.	Mouse	Skeletal muscle
Jelenik 2018 Mol Metab	2018	Jelenik T, Dille M, Müller-Lühlhoff S, Kabra DG, Zhou Z, Binsch C, Hartwig S, Lehr S, Chadt A, Peters EMJ, Kruse J, Roden M, Al-Hasani H, Castañeda TR (2018) FGF21 regulates insulin sensitivity following long-term chronic stress. Mol Metab 16:126-38.	Mouse	Skeletal muscle		Other Diabetes
Goncalves 2018 Neurotoxicology	2018	Gonçalves DF, Courtes AA, Hartmann DD, da Rosa PC, Oliveira DM, Soares FAA, Dalla Corte CL (2018) 6-Hydroxydopamine induces different mitochondrial bioenergetics response in brain regions of rat. Neurotoxicology 70:1-11.	Rat	Nervous system		Parkinson's Neurodegenerative
Larsen 2018 Physiol Rep	2018	Larsen S, Lundby AM, Dandanell S, Oberholzer L, Keiser S, Andersen AB, Haider T, Lundby C (2018) Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males. Physiol Rep 6:e13793.	Human	Skeletal muscle
Nielsen 2018 Neurosci Lett	2018	Nielsen B, Cejvanovic V, Wörtwein G, Hansen AR, Marstal KK, Weimann A, Bjerring PN, Dela F, Poulsen HE, Jørgensen MB (2018) Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue. Neurosci Lett 690:1-5.	Rat	Nervous system		Other
Dogan 2018 Cell Metab	2018	Dogan SA, Cerutti R, Benincá C, Brea-Calvo G, Jacobs HT, Zeviani M, Szibor M, Viscomi C (2018) Perturbed redox signaling exacerbates a mitochondrial myopathy. Cell Metab 28:764-77.	Mouse	Skeletal muscle	Mitochondrial disease	Myopathy
Mavroudis 2018 Eur J Cardiothorac Surg	2018	Mavroudis CD, Karlsson M, Ko T, Hefti M, Gentile JI, Morgan RW, Plyler R, Mensah-Brown KG, Boorady TW, Melchior RW, Rosenthal TM, Shade BC, Schiavo KL, Nicolson SC, Spray TL, Sutton RM, Berg RA, Licht DJ, Gaynor JW, Kilbaugh TJ (2018) Cerebral mitochondrial dysfunction associated with deep hypothermic circulatory arrest in neonatal swine. Eur J Cardiothorac Surg 54:162-68.	Pig	Nervous system	Ischemia-reperfusion Temperature
Trewin 2018 Am J Physiol Regul Integr Comp Physiol	2018	Trewin AJ, Parker L, Shaw CS, Hiam D, Garnham AP, Levinger I, McConell GK, Stepto NK (2018) Acute HIIE elicits similar changes in human skeletal muscle mitochondrial H₂O₂ release, respiration and cell signaling as endurance exercise even with less work. Am J Physiol Regul Integr Comp Physiol 315:R1003-R1016.	Human	Skeletal muscle
Treberg 2018 Redox Biol	2018	Treberg JR, Braun K, Selseleh P (2018) Mitochondria can act as energy-sensing regulators of hydrogen peroxide availability. Redox Biol 20:483-88.	Rat	Skeletal muscle
Pileggi 2018 Free Radic Biol Med	2018	Pileggi CA, Hedges CP, D'Souza RF, Durainayagam BR, Markworth JF, Hickey AJR, Mitchell CJ, Cameron-Smith D (2018) Exercise recovery increases skeletal muscle H₂O₂ emission and mitochondrial respiratory capacity following two-weeks of limb immobilization. Free Radic Biol Med 124:241-8.	Human	Skeletal muscle	Oxidative stress;RONS
Dawson 2018 Acta Physiol (Oxf)	2018	Dawson NJ, Lyons SA, Henry DA, Scott GR (2018) Effects of chronic hypoxia on diaphragm function in deer mice native to high altitude. Acta Physiol (Oxf) 223:e13030.	Other mammals	Skeletal muscle	Hypoxia
Hedges 2018 FASEB J	2018	Hedges CP, Bishop DJ, Hickey AJR (2018) Voluntary wheel running prevents the acidosis-induced decrease in skeletal muscle mitochondrial reactive oxygen species emission. FASEB J 33:4996-5004.	Rat	Skeletal muscle	Oxidative stress;RONS
Kim 2018 Free Radic Biol Med	2018	Kim M, Stepanova A, Niatsetskaya Z, Sosunov S, Arndt S, Murphy MP, Galkin A, Ten VS (2018) Attenuation of oxidative damage by targeting mitochondrial complex I in neonatal hypoxic-ischemic brain injury. Free Radic Biol Med 124:517-24.	Mouse	Nervous system	Ischemia-reperfusion Hypoxia
Jang 2018 J Med Toxicol	2018	Jang DH, Khatri UG, Mudan A, Love JS, Owiredu S, Eckmann DM (2018) Translational application of measuring mitochondrial functions in blood cells obtained from patients with acute poisoning. J Med Toxicol 14:144-51.	Human	Blood cells		Cardiovascular
Falcao-Tebas 2018 J Physiol	2018	Falcão-Tebas F, Kuang J, Arceri C, Kerris JP, Andrikopoulos S, Marin EC, McConell GK (2018) Four weeks of exercise early in life reprograms adult skeletal muscle insulin resistance caused by paternal high fat diet. J Physiol 597:121-36.	Rat	Skeletal muscle		Obesity
Kjeld 2018 PLoS One	2018	Kjeld T, Stride N, Gudiksen A, Hansen EG, Arendrup HC, Horstmann PF, Zerahn B, Jensen LT, Nordsborg N, Bejder J, Halling JF (2018) Oxygen conserving mitochondrial adaptations in the skeletal muscles of breath hold divers. PLoS One 13:e0201401.	Human	Skeletal muscle
Ahn 2018 Redox Biol	2018	Ahn B, Pharaoh G, Premkumar P, Huseman K, Ranjit R, Kinter M, Szweda L, Kiss T, Fulop G, Tarantini S, Csiszar A, Ungvari Z, Van Remmen H (2018) Nrf2 deficiency exacerbates age-related contractile dysfunction and loss of skeletal muscle mass. Redox Biol 17:47-58.	Mouse	Skeletal muscle		Aging;senescence
Logan 2018 Thesis	2018	Logan C (2018) Nitrate and nitrite differentially affect respiration in zebrafish during exercise. Honors Baccalaureate of Science in Nutrition p44.	Zebrafish	Skeletal muscle
Cooper 2018 Exp Physiol	2018	Cooper MA, McCoin C, Pei D, Thyfault JP, Koestler D, Wright DE (2018) Reduced mitochondrial reactive oxygen species production in peripheral nerves of mice fed a ketogenic diet. Exp Physiol 103:1206-12.	Mouse	Nervous system		Obesity Diabetes
Napa 2017 Int J Dent	2017	Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from P. gingivalis negatively alters gingival cell mitochondrial bioenergetics. Int J Dent 2017:2697210.	Human	Endothelial;epithelial;mesothelial cell
De Velasco 2017 Br J Nutr	2017	de Velasco PC, Chicaybam G, Ramos-Filho DM, Dos Santos RMAR, Mairink C, Sardinha FLC, El-Bacha T, Galina A, Tavares-do-Carmo MDG (2017) Maternal intake of trans-unsaturated or interesterified fatty acids during pregnancy and lactation modifies mitochondrial bioenergetics in the liver of adult offspring in mice. Br J Nutr 118:41-52.	Mouse	Liver
Du 2017 Evolution	2017	Du SNN, Khajali F, Dawson NJ, Scott GR (2017) Hybridization increases mitochondrial production of reactive oxygen species in sunfish. Evolution 71:1643-52.	Fishes	Liver	Oxidative stress;RONS Ischemia-reperfusion
Ortega-Dominguez 2017 Food Chem Toxicol	2017	Ortega-Domínguez B, Aparicio-Trejo OE, García-Arroyo FE, León-Contreras JC, Tapia E, Molina-Jijón E, Hernández-Pando R, Sánchez-Lozada LG, Barrera-Oviedo D, Pedraza-Chaverri J (2017) Curcumin prevents cisplatin-induced renal alterations in mitochondrial bioenergetics and dynamic. Food Chem Toxicol 107:373-85.
De Moura 2017 Neurotox Res	2017	de Moura Alvorcem L, da Rosa MS, Glänzel NM, Parmeggiani B, Grings M, Schmitz F, Wyse ATS, Wajner M, Leipnitz G (2017) Disruption of energy transfer and redox status by sulfite in hippocampus, striatum, and cerebellum of developing rats. Neurotox Res 32:264-75.	Rat	Nervous system
Duicu 2017 Can J Physiol Pharmacol	2017	Duicu OM, Privistirescu A, Wolf A, Petruş A, Dănilă MD, Raţiu CD, Muntean DM, Sturza A (2017) Methylene blue improves mitochondrial respiration and decreases oxidative stress in a substrate-dependent manner in diabetic rat hearts. Can J Physiol Pharmacol 95:1376-82.	Rat	Heart		Diabetes Myopathy
Newsom 2017 Am J Physiol Endocrinol Metab	2017	Newsom SA, Miller BF, Hamilton KL, Ehrlicher SE, Stierwalt HD, Robinson MM (2017) Long-term rates of mitochondrial protein synthesis are increased in mouse skeletal muscle with high fat feeding regardless of insulin sensitizing treatment. Am J Physiol Endocrinol Metab 313:552-62.	Mouse	Skeletal muscle		Diabetes Obesity
Sharaf 2017 Biochim Biophys Acta	2017	Sharaf MS, Stevens D, Kamunde C (2017) Mitochondrial transition ROS spike (mTRS) results from coordinated activities of complex I and nicotinamide nucleotide transhydrogenase. Biochim Biophys Acta 1858:955-65.	Mouse Fishes	Heart Liver	Oxidative stress;RONS
Makrecka-Kuka 2017 Toxicol Lett	2017	Makrecka-Kuka M, Volska K, Antone U, Vilskersts R, Grinberga S, Bandere D, Liepinsh E, Dambrova M (2017) Trimethylamine N-oxide impairs pyruvate and fatty acid oxidation in cardiac mitochondria. Toxicol Lett 267:32-8.	Mouse	Heart
Xiong 2017 J Am Heart Assoc	2017	Xiong S, Wang B, Lin S, Zhang H, Li Y, Wei X, Cui Y, Wei X, Lu Z, Gao P, Li L, Zhao Z, Liu D, Zhu Z (2017) Activation of transient receptor potential melastatin subtype 8 attenuates cold-induced hypertension through ameliorating vascular mitochondrial dysfunction. J Am Heart Assoc 6. pii: e005495.	Mouse	Endothelial;epithelial;mesothelial cell	Temperature
Lopez-Manzano 2017 Chem Res Toxicol	2017	Lopez-Manzano E, Cronican AA, Frawley KL, Peterson J, Pearce LL (2017) Cyanide scavenging by a cobalt Schiff-base macrocycle: a cost-effective alternative to corrinoids. Chem Res Toxicol 29:1011-9.
Briston 2017 Sci Rep	2017	Briston T, Roberts M, Lewis S, Powney B, M Staddon J, Szabadkai G, Duchen MR (2017) Mitochondrial permeability transition pore: sensitivity to opening and mechanistic dependence on substrate availability. Sci Rep 7:10492.	Rat	Liver	Permeability transition
Trewin 2017 PLOS ONE	2017	Trewin AJ, Levinger I, Parker L, Shaw CS, Serpiello FR, Anderson MJ, McConell GK, Hare DL, Stepto NK (2017) Acute exercise alters skeletal muscle mitochondrial respiration and H₂O₂ emission in response to hyperinsulinemic-euglycemic clamp in middle-aged obese men. PLOS ONE 12:e0188421.	Human	Skeletal muscle	Oxidative stress;RONS	Obesity
Jang 2017 Biol Open	2017	Jang DH, Seeger SC, Grady ME, Shofer FC, Eckmann DM (2017) Mitochondrial dynamics and respiration within cells with increased open pore cytoskeletal meshes. Biol Open 6:1831-9.	Human	Endothelial;epithelial;mesothelial cell Fibroblast	Oxidative stress;RONS	Cancer
Stepanova 2017 J Cereb Blood Flow Metab	2017	Stepanova A, Kahl A, Konrad C, Ten V, Starkov AS, Galkin A (2017) Reverse electron transfer results in a loss of flavin from mitochondrial complex I: Potential mechanism for brain ischemia-reperfusion injury. J Cereb Blood Flow Metab 37:3649-58.	Mouse	Nervous system	Ischemia-reperfusion
Koning 2017 J Cereb Blood Flow Metab	2017	Koning G, Leverin AL, Nair S, Schwendimann L, Ek J, Carlsson Y, Gressens P, Thornton C, Wang X, Mallard C, Hagberg H (2017) Magnesium induces preconditioning of the neonatal brain via profound mitochondrial protection. J Cereb Blood Flow Metab 39:1038-55.	Rat		Ischemia-reperfusion Hypoxia
Khalifa 2017 Physiol Rep	2017	Khalifa AR, Abdel-Rahman EA, Mahmoud AM, Ali MH, Noureldin M, Saber SH, Mohsen M, Ali SS (2017) Sex-specific differences in mitochondria biogenesis, morphology, respiratory function, and ROS homeostasis in young mouse heart and brain. Physiol Rep 5. pii: e13125.	Mouse	Heart Nervous system		Aging;senescence Cardiovascular Other
Cardoso 2017 Biochim Biophys Acta	2017	Cardoso GMF, Pletsch JT, Parmeggiani B, Grings M, Glanzel NM, Bobermin LD, Amaral AU, Wajner M, Leipnitz G (2017) Bioenergetics dysfunction, mitochondrial permeability transition pore opening and lipid peroxidation induced by hydrogen sulfide as relevant pathomechanisms underlying the neurological dysfunction characteristic of ethylmalonic encephalopathy. Biochim Biophys Acta 1863:2192-2201.	Rat	Nervous system	Permeability transition	Other
Lau 2017 Dissertation	2017	Lau G (2017) Adaptive variation of mitochondrial function in response to oxygen variability in intertidal sculpins (Cottidae, Actinopterygii). Dissertation p148.	Fishes	Nervous system	Hypoxia
Sharaf 2017 Aquat Toxicol	2017	Sharaf MS, Stevens D, Kamunde C (2017) Zinc and calcium alter the relationship between mitochondrial respiration, ROS and membrane potential in rainbow trout (Oncorhynchus mykiss) liver mitochondria. Aquat Toxicol 189:170-83.	Fishes	Liver
Goedecke 2017 JMIR Res Protoc	2017	Goedecke JH, Mendham AE, Clamp L, Nono Nankam PA, Fortuin-de Smidt MC, Phiri L, Micklesfield LK, Keswell D, Woudberg NJ, Lecour S, Alhamud A, Kaba M, Lutomia FM, van Jaarsveld PJ, de Villiers A, Kahn SE, Chorell E, Hauksson J, Olsson T (2017) An exercise intervention to unravel the mechanisms underlying insulin resistance in a cohort of black South African women: Protocol for a randomized controlled trial. JMIR Res Protoc 03/10/2017:9098.	Human	Skeletal muscle Fat		Diabetes Obesity
Power 2016 Am J Physiol Heart Circ Physiol	2016	Power AS, Pham T, Loiselle DS, Crossman DH, Ward ML, Hickey AJ (2016) Impaired ADP channeling to mitochondria and elevated reactive oxygen species in hypertensive hearts. https://doi.org/10.1152/ajpheart.00050.2016	Rat	Heart		Cardiovascular
Abdel-Rahman 2016 Oxid Med Cell Longev	2016	Abdel-Rahman EA, Mokhtar A, Aaliya A, Radwan Y, Yasseen B, Al-Okda A, Atwa A, Elhanafy E, Habashy M, Ali SS (2016) Resolving contributions of oxygen-consuming and ROS-generating enzymes at the synapse. Oxid Med Cell Longev p19.	Mouse	Nervous system	Oxidative stress;RONS
Du 2016 J Exp Biol	2016	Du SN, Mahalingam S, Borowiec BG, Scott GR (2016) Mitochondrial physiology and reactive oxygen species production are altered by hypoxia acclimation in killifish (Fundulus heteroclitus). J Exp Biol 219:1130-8.	Fishes	Liver	Oxidative stress;RONS
Gorbacheva 2016 International Symposium Mitochondrial Motility	2016	Gorbacheva OS, Strutynskyi RB, Khmil NV, Belosludtseva NV, Murzaeva SV, Korobeynikova MO, Alilova GA, Lezhnev EI, Mironova GD (2016) Study of the influence of flocalin on the energy and ion exchanges in rat liver mitochondria. International Symposium Mitochondrial Motility 73-7.	Rat	Liver		Cardiovascular
Bouitbir 2016 Antioxid Redox Signal	2016	Bouitbir J, Singh F, Charles AL, Schlagowski AI, Bonifacio A, Echaniz-Laguna A, Geny B, Krähenbühl S, Zoll J (2016) Statins trigger mitochondrial ROS-induced apoptosis in glycolytic skeletal muscle. Antioxid Redox Signal 24:84-98.	Rat	Skeletal muscle Other cell lines	Oxidative stress;RONS	Cardiovascular Myopathy
Mezera 2016 Oxid Med Cell Longev	2016	Mezera V, Endlicher R, Kucera O, Sobotka O, Drahota Z, Cervinkova Z (2016) Effects of epigallocatechin gallate on tert-butyl hydroperoxide-induced mitochondrial dysfunction in rat liver mitochondria and hepatocytes. Oxid Med Cell Longev 7573131:8pp.	Rat	Liver
Timpani 2016 Neurotherapeutics	2016	Timpani CA, Trewin AJ, Stojanovska V, Robinson A, Goodman CA, Nurgali K, Betik AC, Stepto N, Hayes A, McConell GK, Rybalka E (2016) Attempting to compensate for reduced neuronal nitric oxide synthase protein with nitrate supplementation cannot overcome metabolic dysfunction but rather has detrimental effects in Dystrophin-deficient mdx muscle. Neurotherapeutics 14:429-46.	Mouse	Skeletal muscle		Other
Schiffer 2016 Am J Physiol Cell Physiol	2016	Schiffer TA, Peleli M, Sundqvist ML, Ekblom B, Lundberg JO, Weitzberg E, Larsen FJ (2016) Control of human energy expenditure by cytochrome c oxidase subunit IV-2. Am J Physiol Cell Physiol 311:C452-61.	Human	Skeletal muscle HEK	Oxidative stress;RONS Hypoxia
Larsen 2016 FASEB J	2016	Larsen FJ, Schiffer TA, Ørtenblad N, Zinner C, Morales-Alamo D, Willis SJ, Calbet JA, Holmberg HC, Boushel R (2016) High-intensity sprint training inhibits mitochondrial respiration through aconitase inactivation. FASEB J 30:417-27.	Human	Skeletal muscle	Oxidative stress;RONS
Karlsson 2016 Shock	2016	Karlsson M, Hara N, Morata S, Sjövall F, Kilbaugh T, Hansson MJ, Uchino H, Elmér E (2016) Diverse and tissue-specific mitochondrial respiratory response in a mouse model of sepsis-induced multiple organ failure. Shock 45:404-10.	Mouse	Nervous system Liver	Oxidative stress;RONS	Sepsis
Liepinsh 2016 Biochem J	2016	Liepinsh E, Makrecka-Kuka M, Volska K, Kuka J, Makarova E, Antone U, Sevostjanovs E, Vilskersts R, Strods A, Tars K, Dambrova M (2016) Long-chain acylcarnitines determine ischaemia/reperfusion-induced damage in heart mitochondria. Biochem J 473:1191-202.	Rat	Heart	Ischemia-reperfusion
Baeder 2016 Int J Dent	2016	Baeder AC, Napa K, Richardson ST, Taylor OJ, Andersen SG, Wilcox SH, Winden DR, Reynolds PR, Bikman BT (2016) Oral gingival cell cigarette smoke exposure induces muscle cell metabolic disruption. Int J Dent 2016:2763160.	Mouse	Skeletal muscle
Alleman 2016 Am J Physiol Heart Circ Physiol	2016	Alleman RJ, Tsang AM, Ryan TE, Patteson DJ, McClung JM, Spangenburg EE, Shaikh SR, Neufer PD, Brown DA (2016) Exercise-induced protection against reperfusion arrhythmia involves stabilization of mitochondrial energetics. Am J Physiol Heart Circ Physiol 310:H1360-70.	Rat	Heart	Ischemia-reperfusion	Cardiovascular
Kluckova 2015 Cell Death Dis	2015	Kluckova K, Sticha M, Cerny J, Mracek T, Dong L, Drahota Z, Gottlieb E, Neuzil J, Rohlena J (2015) Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Cell Death Dis 6:e1749.	Other mammals	Lung;gill Other cell lines Fibroblast	Cell death Oxidative stress;RONS
Makrecka-Kuka 2015 Biomolecules	2015	Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319	Human Mouse	Heart Nervous system HEK	Oxidative stress;RONS
Ferrera 2015 J Thorac Cardiovasc Surg	2015	Ferrera R, Benhabbouche S, Da Silva CC, Alam MR, Ovize M (2015) Delayed low pressure at reperfusion: A new approach for cardioprotection. J Thorac Cardiovasc Surg 150:1641-8.	Rat	Heart	Ischemia-reperfusion Permeability transition
Petrus 2015 Can J Physiol Pharmacol	2015	Petruş A, Duicu OM, Sturza A, Noveanu L, Kiss L, Dănilă M, Baczkó I, Muntean DM, Jost N (2015) Modulation of mitochondrial respiratory function and ROS production by novel benzopyran analogues. Can J Physiol Pharmacol 93:811-8.	Rat	Heart	Ischemia-reperfusion
Krumschnabel 2015 Methods Mol Biol	2015	Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. https://doi.org/10.1007/978-1-4939-2257-4_22	Mouse	Nervous system	Oxidative stress;RONS
Beaudoin 2014 J Physiol	2014	Beaudoin MS, Perry CC, Arkell A, Chabowski A, Simpson JA, Wright DC, Holloway GP (2014) In the ZDF rat, impairments in mitochondrial palmitoyl-CoA respiratory kinetics that precede the development of diabetic cardiomyopathy are prevented by resveratrol supplementation. J Physiol 592:2519-33.	Rat	Heart		Diabetes
Pham 2014 Am J Physiol	2014	Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499–507.	Rat	Heart	Ischemia-reperfusion Oxidative stress;RONS Mitochondrial disease	Diabetes Myopathy
Kukat 2014 PLoS Genet	2014	Kukat A, Dogan SA, Edgar D, Mourier A, Jacoby C, Maiti P, Mauer J, Becker C, Senft K, Wibom R, Kudin AP, Hultenby K, Flögel U, Rosenkranz S, Ricquier D, Kunz WS, Trifunovic A (2014) Loss of UCP2 attenuates mitochondrial dysfunction without altering ROS production and uncoupling activity. https://doi.org/10.1371/journal.pgen.1004385	Mouse	Heart	Oxidative stress;RONS
Hunter 2014 PhD Thesis	2014	Hunter FW (2014) Old target; new paradigm: Determinants of sensitivity to hypoxia-directed anticancer prodrugs. PhD Thesis:1-353.	Other mammals	CHO		Cancer
Tretter 2014 Free Radic Biol Med	2014	Tretter L, Horvath G, Hölgyesi A, Essek F, Adam-Vizi V (2014) Enhanced hydrogen peroxide generation accompanies the beneficial bioenergetic effects of methylene blue in isolated brain mitochondria. Free Radic Biol Med 77:317-30.	Guinea pig	Nervous system
Iftikar 2014 Thesis University of Auckland	2014	Iftikar FI (2014) Testing the role of heart mitochondrial stability and function in heart failure of ectotherms exposed to heat stress. Thesis University of Auckland:167pp.	Fishes	Heart	Oxidative stress;RONS Temperature
Soltysinska 2014 PLoS One	2014	Soltysinska E, Bentzen BH, Barthmes M, Hattel H, Thrush AB, Harper ME, Qvortrup K, Larsen FJ, Schiffer TA, Losa-Reyna J, Straubinger J, Kniess A, Thomsen MB, Brüggemann A, Fenske S, Biel M, Ruth P, Wahl-Schott C, Boushel RC, Olesen SP, Lukowski R (2014) KCNMA1 encoded cardiac BK channels afford protection against ischemia-reperfusion injury. PLoS One 9:e103402.	Mouse	Heart Skeletal muscle	Ischemia-reperfusion Oxidative stress;RONS
Hara 2013 Eur J Anaesthesiol	2013	Hara N, Karlsson M, Sjövall F, Hansson Magnus J, Elmér E, Uchino H (2013) Early brain mitochondrial dysfunction in a mouse model of sepsis: 7AP4‐9. Eur J Anaesthesiol 30,112-112.	Mouse	Nervous system	Oxidative stress;RONS	Sepsis
Jelenik 2013 Eur Heart J	2013	Jelenik T, Floegel U, Phielix E, Kaul K, Nowotny P, Partke HP, Schrader J, Roden M, Szendroedi S (2013) Non-alcoholic fatty liver disease and insulin resistance are associated with increased cardiac oxidative stress in mice. Eur Heart J 34:P5045.	Mouse	Heart	Oxidative stress;RONS
Reilly 2013 J Exp Biol	2013	Reilly BD, Hickey AJ, Cramp RL, Franklin CE (2013) Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse. J Exp Biol 217:1087-93.	Amphibians	Heart Skeletal muscle	Oxidative stress;RONS
Iftikar 2013 PLoS One	2013	Iftikar FI, Hickey AJ (2013) Do mitochondria limit hot fish hearts? Understanding the role of mitochondrial function with heat stress in Notolabrus celidotus. PLoS One 8:e64120.	Fishes	Heart	Oxidative stress;RONS
Tretter 2012 Free Radic Biol Med	2012	Tretter Laszlo, Adam-Vizi Vera (2012) High Ca²⁺ load promotes hydrogen peroxide generation via activation of α-glycerophosphate dehydrogenase in brain mitochondria. Free Radic Biol Med 53:2119-30.	Guinea pig	Nervous system	Oxidative stress;RONS
Affourtit 2012 Methods Mol Biol	2012	Affourtit C, Quinlan CL, Brand MD (2012) Measurement of proton leak and electron leak in isolated mitochondria. Methods Mol Biol 810:165-82.	Rat	Skeletal muscle	Oxidative stress;RONS
Hickey 2012 J Comp Physiol B	2012	Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibers of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.	Fishes	Heart	Ischemia-reperfusion Oxidative stress;RONS
Hunter 2012 Biochem Pharmacol	2012	Hunter FW, Wang J, Patel R, Hsu HL, Hickey AJ, Hay MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83:574–85.		CHO	Oxidative stress;RONS
Tretter 2007 J Neurochem	2007	Tretter L, Adam-Vizi V (2007) Uncoupling is without an effect on the production of reactive oxygen species by in situ synaptic mitochondria. J Neurochem 103:1864-71.	Other mammals	Nervous system	Oxidative stress;RONS