Difference between revisions of "Ascorbate"
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== Application in [[HRR]] == | |||
'''As: Ascorbate sodium salt''' (Succinate disodium salt, hexahydrate, C<sub>6</sub>H<sub>7</sub>0<sub>6</sub>Na); Sigma, A 4034, 100 g, store at R.T.): FW = 198.1 | |||
<span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span> | |||
'''Preparation of 0.8 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::1) weigh 1.584 g of Ascorbate sodium salt and dissolve in 10 ml H<sub>2</sub>O | |||
::2) To prevent autooxidation, adjust pH to ca 6 with ascorbic acid (a 137.6 mg ml-1 solution of pH ca 2). | |||
::3) Divide into 0.2 ml portions. | |||
::4) Store frozen at -20 °C. | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
Revision as of 16:47, 9 March 2011
- high-resolution terminology - matching measurements at high-resolution
Ascorbate
Description
Abbreviation: As
MitoPedia topics:
Substrate and metabolite
Application in HRR
As: Ascorbate sodium salt (Succinate disodium salt, hexahydrate, C6H706Na); Sigma, A 4034, 100 g, store at R.T.): FW = 198.1 Caution: Light sensitive (store solution in a dark glass vial).
Preparation of 0.8 M stock solution (dissolved in H2O):
- 1) weigh 1.584 g of Ascorbate sodium salt and dissolve in 10 ml H2O
- 2) To prevent autooxidation, adjust pH to ca 6 with ascorbic acid (a 137.6 mg ml-1 solution of pH ca 2).
- 3) Divide into 0.2 ml portions.
- 4) Store frozen at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12
