Difference between revisions of "Ascorbate"
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|abbr=As | |abbr=As | ||
|description=In respiratory assays for cytochrome c oxidase (COX; [[Complex IV|complex CIV]]) activity, '''ascorbate''' is added as regenerating system for reduced [[TMPD|TMPD]]. | |description=In respiratory assays for cytochrome c oxidase (COX; [[Complex IV|complex CIV]]) activity, '''ascorbate''' is added as regenerating system for reduced [[TMPD|TMPD]]. | ||
|info=MiPNet06.06 | |info=[[MiPNet06.06]] | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics |
Revision as of 07:52, 21 May 2012
Description
In respiratory assays for cytochrome c oxidase (COX; complex CIV) activity, ascorbate is added as regenerating system for reduced TMPD.
Abbreviation: As
Reference: MiPNet06.06
MitoPedia topics:
Substrate and metabolite
Application in HRR
As: Ascorbate sodium salt (Succinate disodium salt, hexahydrate, C6H706Na); Sigma A 4034, 100 g, store at R.T.; FW = 198.1
Caution: Light sensitive (store solution in a dark glass vial).
Preparation of 800 mM stock solution (dissolved in H2O):
- 1) weigh 1.584 g of Ascorbate sodium salt and dissolve in 10 ml H2O
- 2) To prevent autooxidation, adjust pH to ca 6 with ascorbic acid (a 137.6 mg ml-1 solution of pH ca 2).
- 3) Divide into 0.2 ml portions.
- 4) Store frozen at -20 Β°C.
Oxygraph-2k Manual Titrations: MiPNet09.12