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Ascorbate

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Ascorbate

Description

In respiratory assays for cytochrome c oxidase (COX; complex CIV) activity, ascorbate is added as regenerating system for reduced TMPD.

Abbreviation: As

Reference: MiPNet06.06


MitoPedia topics: Substrate and metabolite 

Application in HRR

As: Ascorbate sodium salt (Succinate disodium salt, hexahydrate, C6H706Na); Sigma, A 4034, 100 g, store at R.T.): FW = 198.1

Caution: Light sensitive (store solution in a dark glass vial).

Preparation of 0.8 M stock solution (dissolved in H2O):

1) weigh 1.584 g of Ascorbate sodium salt and dissolve in 10 ml H2O
2) To prevent autooxidation, adjust pH to ca 6 with ascorbic acid (a 137.6 mg ml-1 solution of pH ca 2).
3) Divide into 0.2 ml portions.
4) Store frozen at -20 Β°C.


Oxygraph-2k Manual Titrations: MiPNet09.12