Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Assay"

From Bioblast
(Created page with "{{MitoPedia |abbr=n.a. |description=An experimental '''assay''' is a test run on a single sample. An assay may have ''n'' repetitions for evaluation of procedural variati...")
Β 
Line 1: Line 1:
{{MitoPedia
{{MitoPedia
|abbr=n.a.
|abbr=n.a.
|description=An experimental '''assay''' is a test run on a single [[sample]]. An assay may have ''n'' [[repetition]]s for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point.
|description=An experimental '''assay''' is a test run on a single [[sample]]. An assay may have ''n'' [[repetition]]s for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An [[assay series]] consists of replications of ''N'' assays on different samples.
}}
}}
{{MitoPedia methods
{{MitoPedia methods

Revision as of 06:43, 27 January 2016


high-resolution terminology - matching measurements at high-resolution


Assay

Description

An experimental assay is a test run on a single sample. An assay may have n repetitions for evaluation of procedural variation ('experimental noise') due to instrumental resolution. Subsamples can be obtained (1) from a homogenous sample (e.g. cell suspension, tissue homogenate, isolated mitochondria), (2) as subsamples obtained by splitting a sample into comparable parts (e.g. permeabilized muscle fibres from a biopsy split into different chambers for repeated measurements), or (3) repetitive sampling (e.g. taking multiple biopsies) at a single time point. An assay series consists of replications of N assays on different samples.

Abbreviation: n.a.


MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 

Template:MitoPedia SUIT

MitoPedia topics: Sample preparation