Difference between revisions of "Azide"

Revision as of 12:05, 6 August 2019

high-resolution terminology - matching measurements at high-resolution

Azide

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02

MitoPedia topics: Inhibitor

Azd: Azide (Sodium azide, NaN₃); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.

Caution: Very toxic!

Preparation of 4 M storage and stock solution (dissolved in H₂0):

1) Weigh 260 mg of sodium azide.

2) Dissolve in 1 ml H₂0.

3) Divide into 0.5 ml portions.

4) Store at -20 °C.

O2k manual titrations MiPNet09.12 O2k-Titrations

@@ Line 1: / Line 1: @@
+{{MitoPedia
+|abbr=Azd
+|description='''Sodium azide''' is an inhbitor of [[Complex IV|cytochrome c oxidase]] (COX, CcO).
+|info=[[MiPNet03.02]]
+|type=Inhibitor ETS
+}}
+{{MitoPedia methods|type=Inhibitor ETS
+}}
+{{MitoPedia topics
+|mitopedia topic=Inhibitor
+|type=Inhibitor ETS
+}}== Application in [[HRR]] ==
+'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en&region=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; FW = 65.01.
+<span style="color:#8B008B"> '''Caution:''' Very toxic!</span>
+'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>0):
+::1) Weigh 260 mg of sodium azide.
+::2) Dissolve in 1 ml H<sub>2</sub>0.
+::3) Divide into 0.5 ml portions.
+::4) Store at -20 °C.
+'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::* Titration volume: ≥50 µl using a 50 µl syringe (2 ml O2k-chamber).
+::* Final concentration: ≥100 mM.