Bioblast alert - 2021
Bioblast alert 2021(07) - Diabetes: 2021-04-15
||Diabetes, infarct size, mitochondrial respiration and succinate dehydrogenase inhibition - how do they relate? |
- from the O2k-Network »DK Aarhus Boetker HE«
- Tonnesen PT, Hjortbak MV, Lassen TR, Seefeldt JM, Bøtker HE, Jespersen NR (2021) Myocardial salvage by succinate dehydrogenase inhibition in ischemia-reperfusion injury depends on diabetes stage in rats. Mol Cell Biochem [Epub ahead of print]. - »Bioblast link«
||Gastric bypass surgery improves diabetic nephropathy in type 1 diabetic rats |
- from the O2k-Network »CN Chongqing Zhu Z«
- Wei X, Lu Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic nephropathy through restoration of AMPK activity in type 1 diabetic rats. Cell Rep 32:108207. - »Bioblast link«
||Skeletal muscle mitochondrial bioenergetics in men and women with type 1 diabetes |
- from the O2k-Network »CA Toronto Perry CG«
- Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - »Bioblast link«
Bioblast alert 2021(06) - Fatty acid oxidation (FAO): 2021-03-19
||Approved FAO inhibitor Trimetazidine restricts tumor growth in oxidative lung carcinomas |
- from the O2k-Network »FR Bordeaux Rossignol R«
- Amoedo ND, Sarlak S, Obre E, Esteves P, Bégueret H, Kieffer Y, Rousseau B, Dupis A, Izotte J, Bellance N, Dard L, Redonnet-Vernhet I, Punzi G, Rodrigues MF, Dumon E, Mafhouf W, Guyonnet-Dupérat V, Gales L, Palama T, Bellvert F, Dugot-Senan N, Claverol S, Baste JM, Lacombe D, Rezvani HR, Pierri CL, Mechta-Grigoriou F, Thumerel M, Rossignol R (2021) Targeting the mitochondrial trifunctional protein restrains tumor growth in oxidative lung carcinomas. J Clin Invest 131:e133081. - »Bioblast link«
||Quantifying FAO capacity (F-pathway) - avoid overestimation|
Malate anaplerotic pathways are active in various tissues and cultured cells, which can lead to an overestimation of the F-pathway capacity. .
- Evaluate the malate anaplerotic pathway: »SUIT-027«
- Analyze F-pathway avoiding overestimation: »SUIT-002« and »SUIT-025«
Bioblast alert 2021(05) - Bioenerg Commun & MitoFit Preprints: 2021-03-04
||Mitochondrial physiology in Parkinson’s disease (PD)|
A COST Action MitoEAGLE contribution:
- Krako Jakovljevic N, Ebanks B, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. MitoFit Preprints 2021.4 doi:10.26124/mitofit:2021-0004
||Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?|
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
||Submit manuscripts simultaneously to MitoFit Preprints and Bioenergetics Communications via the BEC website|
A seamless process for taking MitoFit preprints to living BEC communications with open and published peer review.
- »Bioenergetics Communications«
Bioblast alert 2021(04) - MitoFit Preprints: 2021-02-19
||Exploring the Q-junction in real-time|
Simultaneous measurement of coenzyme Q redox state and respiration in isolated mitochondria:
- Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2. doi:10.26124/mitofit:2021-0002
||Diagnosis of mitochondrial disease in frozen samples |
Measurement of respiratory function in cryopreserved muscle biopsies:
- Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. MitoFit Preprints 2021.3. doi:10.26124/mitofit:2021-0003
Bioblast alert 2021(03) - ATP production P»: 2021-01-29
||SUIT protocols with Magnesium Green™ (MgG) can be used to study respiration and ATP production P» |
MgG dye does not affect respiration of cardiac isolated mitochondria.
- Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001
||Measuring ATP-ADP exchange using the fluorophore Magnesium Green |
- from the O2k-Network »HU Budapest Chinopoulos C«
- Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«
||Magnesium Green and P» – which sample preparations work?|
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21
||A quote from MitoPathways Chapter 3. Normalization:
Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s-1∙mg-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s-1∙mg-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s-1∙mg-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.
||Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers |
- from the O2k-Network »AU Melbourne Bishop DJ«
- Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - »Bioblast link«
|| Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers|
- from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«
||What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?|
For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- »Optimum ADP concentration in pfi«
Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05
||The 5th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure – one step beyond ∆Ψ and the protonmotive force.
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