Difference between revisions of "Biological contamination"

Revision as of 10:07, 6 November 2019

Biological contamination

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Biological contamination may be caused by microbial growth in the O2k chamber or in the experimental medium. It should not be confused with other kinds of chamber contamination mentioned in Cleaning the glass chamber.

Biological contamination of the chamber is detected as a high O₂ flux in a closed chamber, measured in an O2k-chamber test: QC2: Instrumental O₂ background.

The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as MiR05. Make sure the 70% ethanol does not contain any additives; e.g., 70 % ethanol used in hospital settings may contain antiseptics (information provided by Garcia-Roves PM). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under Cleaning the glass chamber by removing the glass chamber from the O2k and using a strong acid.

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

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-Biological contamination  is caused by the growth of biological material (originating from samples or otherwise). It should not be confused with other kinds of chamber contamination mentioned in [[Cleaning the glass chamber]].
+:::: Biological contamination may be caused by microbial growth in the O2k chamber or in the experimental medium. It should not be confused with other kinds of chamber contamination mentioned in [[Cleaning the glass chamber]].
-Biological contamination of the chamber is typically characterized by a high O2 flux with a closed chamber near air saturation. It is detected by an [[Oxygen sensor test]], an [[instrumental background test]] or by observing the flux at closed chamber for a short time routinely before any experiment. In the latter two cases the high flux contamination may be caused by biological contamination of the chamber itself or by a contaminated medium. This can be tested by observing the flux in pure water.
+:::: Biological contamination of the chamber is detected as a high O<sub>2</sub> flux in a closed chamber, measured in an O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background''']].
-The ideal remedial agent is 70% ethanol with 30% water (not 100% ethanol). Make sure the 70% ethanol does not contain any additives. E.g. 70% ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]).
+:::: The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the O2k and using a strong acid.
-Prevention of biological contamination of the chamber is primarily by storage under 70% ethanol. If repeated washing with 70% Ethanol does not remove an existing biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the instrument and using a strong acid.
+{{MitoPedia O2k and high-resolution respirometry
-{{#set: Technical service =Chamber}}
+|mitopedia O2k and high-resolution respirometry=O2k-Open Support
-[[Category:Technical service]]
+}}