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Difference between revisions of "Boushel 2011 Mitochondrion"

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(Created page with "{{Publication |title=Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds m...")
 
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{{Publication
{{Publication
|title=Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307. DOI 10.1016/j.mito.2010.12.006
|title=Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307. DOI 10.1016/j.mito.2010.12.006
|info= [http://www.ncbi.nlm.nih.gov/pubmed/21147270 PMID 21147270]  
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21147270 PMID 21147270]
|authors=Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B
|authors=Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B
|year=2011
|year=2011
|journal=Mitochondrion
|journal=Mitochondrion
|mipnetlab=DK Copenhagen Boushel R, AT Innsbruck Gnaiger E
|abstract=Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in-vivo maximal oxygen consumption (''V''O(2)max) is matched to muscle tissue-specific OXPHOS capacity (State 3 respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while in-vivo arm and leg ''V''O(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3±0.4 mmol O(2)kg(-1)min(-1)) was similar to the in-vivo ''V''O(2) during maximal arm cranking (4.7±0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9±0.5 mmol O(2)kg(-1)min(-1), exceeding the in-vivo leg ''V''O(2)max (5.0±0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (''P''<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses in-vivo ''V''O(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans.
|abstract=Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in-vivo maximal oxygen consumption (''V''O(2)max) is matched to muscle tissue-specific OXPHOS capacity (State 3 respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while in-vivo arm and leg ''V''O(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3±0.4 mmol O(2)kg(-1)min(-1)) was similar to the in-vivo ''V''O(2) during maximal arm cranking (4.7±0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9±0.5 mmol O(2)kg(-1)min(-1), exceeding the in-vivo leg ''V''O(2)max (5.0±0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (''P''<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses in-vivo ''V''O(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans.
|mipnetlab=DK_Copenhagen_Boushel R, AT_Innsbruck_Gnaiger E
|discipline=Mitochondrial Physiology
}}
}}
{{Labeling
{{Labeling
|discipline=Mitochondrial Physiology
|instruments=Oxygraph-2k
|organism=Human
|organism=Human
|tissues=Skeletal Muscle
|tissues=Skeletal Muscle
|preparations=Intact Organism, Permeabilized Cell or Tissue; Homogenate
|preparations=Intact Organism, Permeabilized Cell or Tissue; Homogenate
|topics=Respiration; OXPHOS; ETS Capacity, Mitochondrial Biogenesis; Mitochondrial Density
|topics=Respiration; OXPHOS; ETS Capacity, Mitochondrial Biogenesis; Mitochondrial Density
|instruments=Oxygraph-2k
|discipline=Mitochondrial Physiology
}}
}}
* [[Talk:Boushel_2011_Mitochondrion|Selected and evaluated by Max Gassmann, a Member of the Faculty of 1000]]
* [[Talk:Boushel_2011_Mitochondrion|Selected and evaluated by Max Gassmann, a Member of the Faculty of 1000]]

Revision as of 18:36, 10 August 2011

Publications in the MiPMap
Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307. DOI 10.1016/j.mito.2010.12.006

» PMID 21147270

Boushel R, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Mitochondrion

Abstract: Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in-vivo maximal oxygen consumption (VO(2)max) is matched to muscle tissue-specific OXPHOS capacity (State 3 respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while in-vivo arm and leg VO(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3±0.4 mmol O(2)kg(-1)min(-1)) was similar to the in-vivo VO(2) during maximal arm cranking (4.7±0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9±0.5 mmol O(2)kg(-1)min(-1), exceeding the in-vivo leg VO(2)max (5.0±0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (P<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses in-vivo VO(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans.


O2k-Network Lab: DK_Copenhagen_Boushel R, AT_Innsbruck_Gnaiger E


Labels:


Organism: Human  Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Organism"Intact Organism" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Oxygraph-2k