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Boushel 2011 Mitochondrion

From Bioblast
Publications in the MiPMap
Boushel RC, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11: 303-307.

ยป PMID 21147270

Boushel RC, Gnaiger E, Calbet JA, Gonzalez-Alonso J, Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Mitochondrion

Abstract: Across a wide range of species and body mass a close matching exists between maximal conductive oxygen delivery and mitochondrial respiratory rate. In this study we investigated in humans how closely in-vivo maximal oxygen consumption (VO(2)max) is matched to muscle tissue-specific OXPHOS capacity (State 3 respiration. High-resolution respirometry was used to quantify mitochondrial respiration from the biopsies of arm and leg muscles while in-vivo arm and leg VO(2) were determined by the Fick method during leg cycling and arm cranking. We hypothesized that muscle mitochondrial respiratory rate exceeds that of systemic oxygen delivery. OXPHOS capacity of the deltoid muscle (4.3ยฑ0.4 mmol O(2)kg(-1)min(-1)) was similar to the in-vivo VO(2) during maximal arm cranking (4.7ยฑ0.5 mmol O(2)kg(-1)min(-1)) with 6 kg muscle. In contrast, the mitochondrial OXPHOS capacity of the quadriceps was 6.9ยฑ0.5 mmol O(2)kg(-1)min(-1), exceeding the in-vivo leg VO(2)max (5.0ยฑ0.2mmolO(2)kg(-1)min(-1)) during leg cycling with 20 kg muscle (P<0.05). Thus, when half or more of the body muscle mass is engaged during exercise, muscle mitochondrial respiratory capacity surpasses in-vivo VO(2)max. The findings reveal an excess capacity of muscle mitochondrial respiratory rate over O(2) delivery by the circulation in the cascade defining maximal oxidative rate in humans.


โ€ข O2k-Network Lab: DK_Copenhagen_Boushel RC, AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom


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Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Intact Organism"Intact Organism" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized tissue 

Regulation: Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.  Coupling state: OXPHOS 

HRR: Oxygraph-2k