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Difference between revisions of "Calisto F"

From Bioblast
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****: [[Short-Term_Scientific_Missions_MitoEAGLE#STSM_Grant_Period_3 |STSM Grant Period 3]]
****: [[Short-Term_Scientific_Missions_MitoEAGLE#STSM_Grant_Period_3 |STSM Grant Period 3]]
::: '''Work Plan summary'''
::: '''Work Plan summary'''
:::: I would like to apply to a short-term scientific mission grant, which will allow me to understand the electron transfer pathway and the proton translocation mechanism of Alternative Complex III. I am a PhD student at ITQB in the Biological Energy Transduction group under the supervision of Manuela M. Pereira. I am dedicated to the study of the Alternative Complex III (ACIII), a protein from bacteria respiratory chain, which has the same quinol:cytochrome c oxidoreductase activity as the cytochrome bc1 complex. Recently, I published as first coauthor an article on the structural characterization of ACIII (Nat Commun. 2018 doi: 10.1038/s41467-018-04141-8) where we identified two putative proton pathways and one quinol-binding. These results provide the first direct evidence that ACIII operates by a redox-driven proton translocation mechanism, totally unrelated to the Q-cycle of cytochrome bc1 complex. In order to extend this characterization, I would like to test the proton pump activity of ACIII in liposomes, using fluorescence and advance membrane protein purification/reconstitution methodologies pioneered in Dr Duncan G.G. McMillan Lab at the Department of Biotechnology, Delft University of Technology. Thank you for considering my application. Sincerely, Filipa Calisto





Revision as of 10:56, 23 October 2018


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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


Calisto F


MitoPedia topics: EAGLE 

COST: Member


Name Calisto Filipa, PhD student
Institution Biological Energy Transduction Group

ITQB-UNL

Address Av. da República

Estação Agronómica Nacional, 2780-157

City Oeiras
State/Province
Country Portugal
Email fcalisto@itqb.unl.pt
Weblink http://www.itqb.unl.pt/bet
O2k-Network Lab


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MitoEAGLE Short-Term Scientific Mission

Work Plan summary
I would like to apply to a short-term scientific mission grant, which will allow me to understand the electron transfer pathway and the proton translocation mechanism of Alternative Complex III. I am a PhD student at ITQB in the Biological Energy Transduction group under the supervision of Manuela M. Pereira. I am dedicated to the study of the Alternative Complex III (ACIII), a protein from bacteria respiratory chain, which has the same quinol:cytochrome c oxidoreductase activity as the cytochrome bc1 complex. Recently, I published as first coauthor an article on the structural characterization of ACIII (Nat Commun. 2018 doi: 10.1038/s41467-018-04141-8) where we identified two putative proton pathways and one quinol-binding. These results provide the first direct evidence that ACIII operates by a redox-driven proton translocation mechanism, totally unrelated to the Q-cycle of cytochrome bc1 complex. In order to extend this characterization, I would like to test the proton pump activity of ACIII in liposomes, using fluorescence and advance membrane protein purification/reconstitution methodologies pioneered in Dr Duncan G.G. McMillan Lab at the Department of Biotechnology, Delft University of Technology. Thank you for considering my application. Sincerely, Filipa Calisto


MitoEAGLE Inclusiveness Target Countries - Conference Grant

Scientific report - EBEC2018