Difference between revisions of "Carboxyatractyloside"
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=Cat | |abbr=Cat | ||
|description='''Carboxyatractyloside''' is an inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. | |description='''Carboxyatractyloside''' is an inhibitor of the [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]]. It is a highly selective inhibitor of cytosolic side-specific mitochondrial ADP/ATP carrier; i.e. adenine nucleotide translocase (ANT); causes stabilization of the ''c'' conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis. | ||
|info=[[MiPNet03.02]] | |info=[[MiPNet03.02]] | ||
|type=Inhibitor PS | |type=Inhibitor PS | ||
| Line 11: | Line 11: | ||
|type=Inhibitor PS | |type=Inhibitor PS | ||
}} | }} | ||
Cat is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition. | |||
== Application in [[HRR]] == | |||
'''Cat: Carboxyatractyloside''' (Carboxyatractyloside potassium salt from ''Xanthium sibiricum'', C<sub>31</sub>H<sub>44</sub>O<sub>18</sub>S<sub>2</sub>*K<sub>x</sub>); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); '''Alternative source''': Carboxyatractyloside potassium salt from ''Xanthium sibiricum'' (C<sub>31</sub>H<sub>43</sub>O<sub>18</sub>S<sub>2</sub>K<sub>3</sub>*3H<sub>2</sub>O) Merck / Calbiochem 216291-5MG, 5 mg, store at -20 °C, FW = 939.1 | |||
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!</span> | |||
'''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>0): | |||
::1) weigh 4.5 mg of Carboxyatractyloside | |||
::2) dissolve in 1 ml H<sub>2</sub>O | |||
::3) divide into 0.2 ml aliquots | |||
::4) store frozen at -20 °C | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 30 µl using a 50 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 0.75 mM | |||
'''Comment:''' Cat is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition. | |||
Cat is available from Sigma (C4992; only 2 mg available), Calbiochem (216201-2MG or 216201-5MG). | Cat is available from Sigma (C4992; only 2 mg available), Calbiochem (216201-2MG or 216201-5MG). | ||
* [[Talk:Carboxyatractyloside]] | * [[Talk:Carboxyatractyloside]] | ||
Revision as of 08:49, 18 May 2012
- high-resolution terminology - matching measurements at high-resolution
Carboxyatractyloside
Description
Carboxyatractyloside is an inhibitor of the Adenine nucleotide translocator (ANT). It is a highly selective inhibitor of cytosolic side-specific mitochondrial ADP/ATP carrier; i.e. adenine nucleotide translocase (ANT); causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.
Abbreviation: Cat
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Cat: Carboxyatractyloside (Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H44O18S2*Kx); Sigma C 4992, 2 mg, store at -20 °C; FW = 802.99 (free acid basis); Alternative source: Carboxyatractyloside potassium salt from Xanthium sibiricum (C31H43O18S2K3*3H2O) Merck / Calbiochem 216291-5MG, 5 mg, store at -20 °C, FW = 939.1
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. Very toxic!
Preparation of 5 mM stock solution (dissolved in H20):
- 1) weigh 4.5 mg of Carboxyatractyloside
- 2) dissolve in 1 ml H2O
- 3) divide into 0.2 ml aliquots
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 30 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.75 mM
Comment: Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.
Cat is available from Sigma (C4992; only 2 mg available), Calbiochem (216201-2MG or 216201-5MG).
* Talk:Carboxyatractyloside
